[目的]丙泊酚是一種中樞神經(jīng)系統(tǒng)抑制劑,在體內(nèi)條件下劑量依賴(lài)性的抑制大腦新皮層的信息處理。然而,丙泊酚對(duì)活體動(dòng)物小腦皮質(zhì)分子層感覺(jué)信息處理的影響還不清楚。因此,我們?cè)跒趵孤樽砺樽硐?應(yīng)用電生理學(xué)和藥理學(xué)方法研究丙泊酚對(duì)感覺(jué)刺激誘發(fā)小鼠小腦分子層場(chǎng)電位反應(yīng)的影響。[方法]6-8周齡的成年ICR小鼠通過(guò)腹腔注射體重為1.3g/kg烏拉坦麻醉后,進(jìn)行氣管插管以避免呼吸道堵塞,然后將小鼠固定在定制的立體定位裝置上。在小腦相應(yīng)部位作一個(gè)防水外流灌流槽后,在小腦Crus Ⅱ區(qū)的相對(duì)應(yīng)部位行開(kāi)顱手術(shù),鉆一個(gè)直徑為1-1.5mm的小孔,暴露記錄部位的小腦表面,并用蠕動(dòng)泵在暴露腦表面持續(xù)灌流含氧的人工腦脊液（artificial cerebrospinal fluid, ACSF）o使用Axopatch-200B膜片鉗放大器（Molecular Devices, Foster City, CA）記錄感覺(jué)刺激誘發(fā)小腦皮層分子層場(chǎng)電位,并通過(guò)D/A轉(zhuǎn)換器1440和Clampex 10.3軟件獲取感覺(jué)刺激誘發(fā)場(chǎng)電位活動(dòng)的數(shù)據(jù)。記錄電極內(nèi)填充的是人工腦脊液,阻抗在3-5 MΩ之間。丙泊酚和gabazine購(gòu)置... 

【文章來(lái)源】：延邊大學(xué)吉林省 211工程院校

【文章頁(yè)數(shù)】：89 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
英文縮寫(xiě)詞表
Chapter 1 Propofol modulates sensory information processing in cerebellar molecular layerin vivo in mice
    Abstract
    摘要
    1.1 Introduction
    1.2 Experimental procedure
        1.2.1 Anesthesia and surgical procedures
        1.2.2 Electrophysiological recordings and drug application
        1.2.3 Statistical analysis
    1.3 Results
        1.3.1 Effects of propofol on the facial stimulation-evoked field potential responsesin cerebellar molecular layer
A receptoractivity">        1.3.2 Propofol affected sensory responses via the modulation of GABA_A receptoractivity
    1.4 Discussion
        1.4.1 Propofol inhibited the sensory stimulation evokes GABAergic component offield potential responses in cerebellar molecular layer
        1.4.2 Propofol enhanced the sensory stimulation evokes excitatory component offield potential responses in cerebellar molecular layer
A receptor">        1.4.3 Propofol modulated the sensory stimulation-evoked field potentialresponses in cerebellar molecular layer via GABA_A receptor
    1.5 Conclusion
    Acknowledgement
    References
Chapter 2 Propofol enhances facial stimulation-evoked responses in
    Abstract
    摘要
    2.1 Introduction
    2.2 Experimental procedure
        2.2.1 Anesthesia and surgical procedures
        2.2.2 Electrophysiological recordings and drug application
        2.2.3 Chemicals
        2.2.4 Statistical analysis
    2.3 Results
        2.3.1 Effects of propofol on the facial stimulation-evoked field potential responsesin cerebellar granule cell layer
on in cerebellar granule cell layer">        2.3.2 Blocking GABAa receptor activity failed to prevent the propofol enhancedfacial stimulation-evoked R_on in cerebellar granule cell layer
on, and abolished the propofol-induced enhancement of R_on">        2.3.3 Blockade of NMDA receptors activity attenuated facial stimulation-evokedR_on, and abolished the propofol-induced enhancement of Ron

    2.4 Discussion
        2.4.1 P roperties of sensory information transferred by cerebellar granule cell
        2.4.2 Propofol affects sensory information transferring in cerebellar granule
A receptor">        2.4.3 Propofol enhanced facial stimulation evoked responses in granule cell layervia modulation of NMDA receptor, but not GABA_A receptor
    2.5 Conclusions
    Acknowledgement
    References
Review:Mechanisms of propofol modulate neuronal activity and synaptictransmission
    References
在讀博士期間申請(qǐng)到的課題、獲得的獎(jiǎng)項(xiàng)及發(fā)表的論文
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