【摘要】：目的：本研究采用長白豬多器官功能障礙綜合征（MODS）模型，通過實驗研究，探討中藥血必凈防治高原多器官功能障礙綜合征（H-MODS）的療效及其機制，為臨床應用提供實驗依據(jù)。 方法：選用體質量為10～15kg的2月齡健康雄性長白豬24只，于蘭州（海拔1520m）適應性喂養(yǎng)1周后，急進至�？撇菰ê０�3200m）行動物實驗。所有長白豬麻醉后，隨機分為空白對照組（A組）、模型組（B組）、血必凈低劑量組（C組）、血必凈高劑量組（D組），每組6只。B組、C組、D組分別經(jīng)耳緣靜脈滴注內毒素（LPS），總量0.35mg/kg，制作MODS模型。內毒素輸畢后，C組給予：血必凈注射液1.5mL/kg，2/日；D組給予：血必凈注射液3mL/kg，2/日； A、B組給予等體積生理鹽水，2/日；共治療3天。于不同時間段觀察各組長白豬的生命體征、臟器功能指標（丙氨酸氨基轉移酶（ALT）、天門冬氨酸氨基轉移酶（AST）、總膽紅素（TBIL）、肌酐（CR））、炎性因子（TNF-α、IL-1）及臟器病理改變，對比各組的統(tǒng)計學差異及組內各時間段的變化規(guī)律，，得出結論。實驗數(shù)據(jù)應用SPSS17.0統(tǒng)計分析軟件包進行分析。 結果：1. B、C、D組體溫、呼吸、心率明顯升高，與A組相比，差異有統(tǒng)計學意義（P0.05或P0.01）；B組呼吸、心率較C、D組升高，差異有統(tǒng)計學意義（P0.05,P0.05）；24h B組死亡率為33.3%，C、D組死亡率均為16.6%。2. B、C、D組血白細胞（WBC）呈“勺型”變化。B組變化幅度較C、D組明顯（P0.05或P0.01）。48h時，D組WBC升高幅度較C組低（P0.05）。3. B、C、D組AST、ALT、TBIL、CR于6h均較A組明顯上升，差異有統(tǒng)計學意義（P0.05或P0.01），至48h達到峰值，以B組升高最明顯，與C、D組比較，差異有統(tǒng)計學意義（P0.05或P0.01），后逐漸下降；24h后，D組AST、CR升高幅度較C組低（P0.05, P0.05）。4.與A組相比，B、C、D組TNF-α、IL-1表達于6h明顯升高（均為P0.01），12h達到峰值（均為P0.01），以B組上升趨勢最明顯，與C、D組相比，差異有統(tǒng)計學意義（P0.05或P0.01），后逐漸下降。D組24h TNF-α及48h IL-1表達均低于C組，差異顯著（P0.05, P0.05）；24h B、C、D組NF-κBmRNA表達量明顯高于A組，差異有統(tǒng)計學意義（均為P0.01），以B組升高幅度最大；72h時，B組NF-κBmRNA表達量仍高于A組（P0.05），而C、D組表達量已接近A組水平。 結論：血必凈注射液干預治療內毒素誘導的高原長白豬MODS模型，不僅可降低其死亡率，減輕內毒素所致的肝、腎功能損傷，而且可以阻斷炎性介質的釋放，降低炎性因子對機體產(chǎn)生的刺激作用，提高H-MODS的生存率，表明血必凈對H-MODS有良好的防治作用。
[Abstract]:Objective: to investigate the effect and mechanism of Xuebijing, a Chinese medicine, on preventing and treating multiple organ dysfunction syndrome (H-MODS) at high altitude by using (MODS) model of Landrace pig multiple organ dysfunction syndrome. To provide experimental basis for clinical application. Methods: 24 healthy 2-month-old male Landrace pigs with body mass of 10~15kg were fed adaptively in Lanzhou (1520m above sea level) for one week, and then the animal experiment was carried out in the mulberry steppe (3200m above sea level). After anesthesia, all Landrace pigs were randomly divided into control group (group A), model group (group B), low dose group of Xuebijing (group C) and high dose group of Xuebijing (group D). Group D was given endotoxin (LPS), 0.35 mg / kg via ear vein to make MODS model. After endotoxin infusion, group C was given Xuebijing injection 1.5 mL / kg / d, group D was given 3 mL 路kg -1 路kg -1 / d of Xuebijing injection, and group A B was given saline of the same volume, 2 days, for 3 days. The vital signs and organ function indexes (alanine aminotransferase (ALT),) aspartate aminotransferase (AST),) total bilirubin (TBIL), creatinine (CR), inflammatory factor (TNF- 偽) were observed in different periods of time. IL-1) and pathological changes of viscera. The experimental data were analyzed by SPSS17.0 statistical analysis software package. Results: 1. The body temperature, respiration and heart rate in group D were significantly higher than those in group A (P0.05 or P0.01); B, P 0.05). The mortality rate of group B was 33.3% and that of group D was 16.60.2. The change of leukocyte (WBC) in group B was significantly higher than that in group C (P0.05 or P0.01). At 48 h, the increase of WBC in group D was lower than that in group C (P0.05). The AST,ALT,TBIL,CR in group B was significantly higher than that in group A at 6 h (P0.05 or P0.01), and reached its peak value at 48 h, especially in group B, which was significantly higher than that in group C (P 0.05 or P0.01), and was significantly higher than that in group C (P < 0.05). The difference was statistically significant (P0.05 or P0.01), and then decreased gradually. After 24 hours, the increase of AST,CR in group D was lower than that in group C (P 0.05, P 0.05). Compared with group A, the expression of TNF- 偽 and IL-1 in group B was significantly higher than that in group A at 6 h (all P0.01), and reached a peak at 12 h (P0.01). The expression of TNF- 偽 and IL-1 at 24 h and 48 h in group D were lower than those in group C (P0.05 or P0.01), and the difference was significant (P0.05, P0.05). The expression of NF- 魏 BmRNA in group B was significantly higher than that in group A (P0.01). At 72 h, the expression of NF- 魏 BmRNA in group B was still higher than that in group A (P0.05), while the expression of NF- 魏 BmRNA in group C was close to the level of group A. Conclusion: the intervention of Xuebijing injection in the treatment of MODS model induced by endotoxin can not only reduce the mortality, alleviate the damage of liver and kidney function induced by endotoxin, but also block the release of inflammatory mediators. Reducing the stimulating effect of inflammatory factors on the body and increasing the survival rate of H-MODS showed that Xuebijing had a good preventive and therapeutic effect on H-MODS.
【學位授予單位】：甘肅中醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R259;R285.5

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