發(fā)布時間：2018-06-21 09:53

  本文選題：海馬CA1區(qū) + 興奮性突觸后電流　； 參考：《新疆醫(yī)科大學》2014年碩士論文

【摘要】：目的：觀察靜脈麻醉藥物依托咪酯對大鼠海馬CA1區(qū)興奮性突觸后電流(excitatory postsynaptic current, EPSC)及離子通道的影響,探索全麻原理及中樞作用機制。方法：10只成年雄性Wistar大鼠迅速斷頭后分離海馬組織,運用低溫切片機制作400gm厚度的海馬腦切片40片,選擇完整均勻的腦片20片(n=20),遂隨機分為2組置于60μl脂肪乳劑(n=10)或10μmol/L依托咪酯乳劑(n=10)中培養(yǎng)40分鐘,后置入膜片鉗載物槽中,在Schaeffer側支／聯合纖維處給予單刺激(刺激強度0.1nA),間隔20s后改變膜鉗制電位(-100mV～+40mV,變化幅度為20mV),再次給予單刺激,記錄翻轉電位,EPSC幅值與頻率,采用細胞貼附式膜片鉗記錄技術全程記錄單通道氯電流頻譜。結果：保持膜鉗制電位在-60mV時,脂肪乳劑組與依托咪酯組相比,氯離子翻轉電位由-53mV左右降低為-60mV左右；全程中EPSC幅值、發(fā)生頻率較脂肪乳劑組均降低(P0.05)；依托咪酯組單通道氯電流發(fā)生明顯增強,通道開放程度增加(P0.05)。結論：依托咪酯主要作用于突觸后膜GABA受體,通過抑制EPSC的發(fā)生和程度達到抑制性突觸傳導的作用;降低膜翻轉電位,使膜電位超極化；此過程中離子內流的程度增加,陰離子通道開放概率升高,這種膜內外環(huán)境的改變可能為氯離子的跨膜轉運所造成。
[Abstract]:Aim: to observe the effect of etomidate on excitatory postsynaptic current (EPSCT) and ion channel in hippocampal CA1 region of rats, and to explore the mechanism of general anesthesia. Methods Hippocampal tissue was isolated from 10 adult male Wistar rats after decapitation, and 40 slices of 400gm thick hippocampal brain were made by hypothermia slicing machine. 20 slices of brain slices were randomly divided into two groups, which were cultured in 60 渭 l fat emulsion or 10 渭 mol / L etomidate emulsion for 40 minutes, then placed in the patch clamp slot. The Schaeffer lateral branch / combined fibers were given single stimulation (stimulation intensity 0.1 nAN, interval 20 s). The membrane clamping potential was changed from 100 MV to 40 MV, and the amplitude of change was 20 MV. The amplitude and frequency of EPSC were recorded, and the amplitude and frequency of EPSC were recorded. Single channel chlorine current spectrum was recorded by cell attached patch clamp recording technique. Results: compared with etomidate group, the chloride ion turnover potential of fat emulsion group was decreased from -53 MV to -60 MV, the amplitude of EPSC and the frequency of occurrence were lower than that of fat emulsion group (P 0.05). In etomidate group, the single channel chlorine current was significantly increased, and the opening degree of the channel was increased (P 0.05). Conclusion: etomidate mainly acts on GABA receptor of postsynaptic membrane, which inhibits synaptic conduction by inhibiting the occurrence and degree of EPSC, reduces membrane turnover potential and makes membrane potential hyperpolarization, and increases the degree of ion influx during this process. The opening probability of anion channel is increased, and the change of the environment inside and outside the membrane may be due to the transport of chloride ions across the membrane.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R614

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本文編號：2048182