本文選題：認知障礙 + 應激免疫反應��； 參考：《山東中醫(yī)藥大學》2014年碩士論文

【摘要】：目的：通過麻醉行脾切除術建立POCD大鼠模型，探討POCD的發(fā)病機制，以及評價術后應用四逆湯與多奈哌齊對POCD的療效。 方法：選取符合實驗要求的健康老齡雄性SD大鼠48只，體重550-650g，隨機分為3組，為四逆湯組(A組)、多奈哌齊組(B組)和對照組(C組)。術前連續(xù)六天進行水迷宮訓練，曠場試驗。在相同的條件下用異氟醚麻醉行脾切除術。麻醉過程持續(xù)4h。術后待大鼠清醒后給予相應的藥物灌胃，術后注意保溫。術后連續(xù)灌胃七天。術后1d行水迷宮及曠場試驗，于術前1天、術后即刻、術后24h靜脈取血。術后3，7，21d只行水迷宮及曠場試驗。最后取腦并切片，用免疫組化法檢測β-AP的表達。抽取的血液進行ACTH、ALD、TNF-α水平及NSE、S-100β的含量測定。 結果：手術過程中大鼠生命體征平穩(wěn)，水迷宮實驗結果表明，手術結束后第1、3天，A組大鼠逃避潛伏期明顯短于B組和C組(P0.01)。曠場實驗結果：術后1d，A組的跨格總數(shù)、站立次數(shù)、排便次數(shù)與另兩組相比具有明顯差異，均具有統(tǒng)計學意義。而對于TNF-α，ACTH，ALD，NSE，S100β指標，三組較術前均有增加，C組較A、B組的增加更明顯；β-AP的沉積亦如此。 結論：結果術后應用四逆湯可有效抑制術后應激反應的發(fā)生，減少大腦中β-淀粉樣蛋白的沉積；降低術后認知障礙發(fā)生率；術后應用多奈哌齊可增加大鼠腦海馬區(qū)膽堿乙酰轉移酶及煙堿型乙酰膽堿受體的表達，，亦可減少大腦中β一淀粉樣蛋白的沉積，對術后認知功能障礙可起到一定的防治作用。
[Abstract]:Objective: to establish a rat model of POCD by splenectomy under anesthesia, to explore the pathogenesis of POCD and to evaluate the effect of Sini decoction and Donepezil on POCD after operation. Methods: 48 male Sprague-Dawley (SD) rats, weighing 550-650 g, were randomly divided into three groups: Sini decoction group (group A), Donepezil group (group B) and control group (group C). Water maze training and open field test were performed for 6 days before operation. Splenectomy was performed with isoflurane anesthesia under the same conditions. The anesthesia lasted for 4 hours. After operation, the rats were given the appropriate medicine after waking up, and the heat preservation was noticed after operation. Postoperative gastric perfusion for 7 days. Water maze test and open field test were performed 1 day after operation, 1 day before operation, immediately after operation and 24 hours after operation. Water labyrinth and open field test were performed only 3 days and 7 days after operation. Finally, the expression of 尾-AP was detected by immunohistochemical method. The level of TNF- 偽 and the content of NSES-100 尾 in ACTHG ALDN were determined. Results: the vital signs of rats were stable during the operation. The results of water maze test showed that the escape latency of rats in group A was significantly shorter than that in group B and group C on the 1st day after operation. The results of open field experiment showed that there were significant differences in the total number of translattice, standing times and defecation times between group A and the other two groups on the 1st day after operation, all of which were statistically significant. But for TNF- 偽 ACTHN, NSES100 尾, the increase of S100 尾 in all three groups was more obvious than that in group A B, and so was the deposition of 尾 -AP. Conclusion: the post-operative application of Sini decoction can effectively inhibit the occurrence of post-operative stress response, reduce the accumulation of 尾 -amyloid protein in the brain, and reduce the incidence of postoperative cognitive impairment. The expression of choline acetyltransferase and nicotinic acetylcholine receptor and 尾 -amyloid protein deposition in the brain of rats were increased after operation with Donepezil, which may play a role in the prevention and treatment of postoperative cognitive dysfunction.
【學位授予單位】：山東中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R619

【參考文獻】

相關期刊論文 前1條

1 吳文春;董敏;曹雅軍;;老年患者圍手術期NSE、S-100β蛋白水平變化與POCD的關系[J];臨床醫(yī)學工程;2012年10期

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