本文選題：腫瘤壞死因子α + 根尖周炎　； 參考：《大連醫(yī)科大學》2014年碩士論文

【摘要】：目的：研究糞腸球菌在大鼠根尖周炎過程中對腫瘤壞死因子α（TNF-α）的表達影響。 方法：選取健康雄性SD大鼠24只，右側(cè)下頜第一磨牙開髓后，，隨機分為A、B兩組，每組各12只。A組在髓腔內(nèi)封入PBS緩沖液棉球,設(shè)為未封菌組；B組在髓腔內(nèi)封入糞腸球菌與PBS緩沖液調(diào)制的菌懸液棉球,設(shè)為封菌組。兩組均以玻璃離子粘固劑封閉髓腔，調(diào)牙合；健康的左側(cè)下頜第一磨牙為空白對照組。A、B兩組分別于術(shù)后1周、2周、3周、4周四個時間點隨機各取3只大鼠，多聚甲醛麻醉，去除軟組織，分離下頜骨，用挖匙分別刮取右側(cè)下頜第一磨牙和左側(cè)下頜第一磨牙根尖周組織，提取組織RNA，逆轉(zhuǎn)錄成cDNA，采用實時熒光定量PCR(Real-timePCR)手段檢測各組TNF-αmRNA的表達水平并進行統(tǒng)計學分析。 結(jié)果： 1.TNF-αmRNA在空白對照組有少量表達。A組和B組TNF-αmRNA表達量與空白對照組比均增高，差異具有統(tǒng)計學意義(p＜0.05)。 2.A組術(shù)后，1周到2周TNF-αmRNA表達量逐漸增高，2周達到最高，3周下降，4周繼續(xù)下降。其中2周與3周相比，差異有統(tǒng)計學意義(p＜0.05)。 3.B組術(shù)后，1周到2周TNF-αmRNA表達量逐漸增高，到2周達到高峰，差異有統(tǒng)計學意義(p＜0.05)。2周到4周，TNF-αmRNA表達量呈下降趨勢差異有統(tǒng)計學意義(p＜0.05)。 4.B組TNF-α mRNA表達量高于A組，差異有統(tǒng)計學意義(p＜0.05)。 結(jié)論： 1.TNF-α mRNA的表達水平與根尖周炎密切相關(guān)，提示TNF-α參與根尖周炎的炎癥和破骨過程。 2.TNF-α與糞腸球菌感染為主的根尖周炎密切相關(guān)，提示糞腸球菌可能加重了根尖周組織的炎癥反應(yīng)和破骨。
[Abstract]:Objective: To study the effect of Enterococcus faecalis on expression of tumor necrosis factor alpha (TNF- alpha) in periapical periodontitis of rats.
Methods: 24 healthy male SD rats were selected, and the right mandibular first molar was randomly divided into A and B two groups. 12.A groups each in each group were sealed into the PBS buffer solution cotton ball in the medullary cavity. The B group was sealed in the medullary cavity with Enterococcus faecalis and PBS buffer liquid cotton balls. The two groups were treated with glass ion cements. The healthy left mandibular first molar was the blank control group.A, and the B two groups were randomly selected from 3 rats at 1 weeks, 2 weeks, 3 weeks and 4 Thursday time respectively. The paraformaldehyde was used to remove the soft tissue and the mandible, and the right mandibular first molars and the left mandibular first molar root periapical tissues were scraped with the dug spoon respectively. The tissue RNA was extracted and transcribed into cDNA. The expression level of TNF- alpha mRNA was detected by real-time fluorescence quantitative PCR (Real-timePCR) and analyzed statistically.
Result錛

本文編號：1942923