本文選題：遞增負(fù)荷 + 自由基��； 參考：《河北師范大學(xué)》2017年碩士論文

【摘要】：研究目的:在遞增負(fù)荷至力竭運(yùn)動(dòng)中機(jī)體內(nèi)活性氧(ROS)與血液中乳酸(LD)有著類似突增點(diǎn)的動(dòng)態(tài)變化規(guī)律,且濃度變化與運(yùn)動(dòng)負(fù)荷有密切關(guān)系。ROS突增點(diǎn)(閾)表示ROS生成與抗氧化清除的失衡點(diǎn),對(duì)氧化損傷監(jiān)控有重要的應(yīng)用價(jià)值。本文通過對(duì)完成同等條件遞增負(fù)荷運(yùn)動(dòng)的無訓(xùn)練組和有訓(xùn)練組大鼠進(jìn)行對(duì)比,驗(yàn)證ROS閾的客觀存在性,并觀察有訓(xùn)練組與無訓(xùn)練組大鼠相比在完成同等條件遞增負(fù)荷運(yùn)動(dòng)時(shí)血液和骨骼肌中ROS閾是否會(huì)出現(xiàn)后移的的現(xiàn)象,同時(shí)觀察血液中LD與肌酸激酶(CK)的動(dòng)態(tài)變化,為深入探討機(jī)制提供實(shí)驗(yàn)證據(jù)。研究方法:選取6周齡雄性Wistar大鼠42只,隨機(jī)分為無運(yùn)動(dòng)訓(xùn)練組(E6組,再分為6個(gè)亞組,每亞組3只,共18只);有氧運(yùn)動(dòng)訓(xùn)練組(E8組,再分為8個(gè)亞組,每亞組3只,共24只,進(jìn)行3周游泳訓(xùn)練)。E8組游泳訓(xùn)練結(jié)束24小時(shí)后,兩組大鼠進(jìn)行同等條件的一次性遞增負(fù)荷力竭游泳運(yùn)動(dòng),第1級(jí)負(fù)荷從無負(fù)重開始,之后每級(jí)負(fù)荷增加大鼠體重的1%,每級(jí)游泳5min。E6組設(shè)6級(jí)負(fù)荷,E8組設(shè)8級(jí)負(fù)荷。各亞組大鼠完成相應(yīng)負(fù)荷后即刻麻醉,取血,取比目魚肌、紅腓腸肌、白腓腸肌,試劑盒檢測(cè)血液和肌組織中的丙二醛(MDA)、H_2O_2、LD含量和CK、SOD活力。研究結(jié)果:1、兩組大鼠在完成同等條件的遞增負(fù)荷運(yùn)動(dòng)時(shí),血液的MDA、H_2O_2、LD含量及SOD、CK活力變化:(1)血液中MDA、H_2O_2含量及CK活力E6組中第6級(jí)負(fù)荷與第5級(jí)負(fù)荷相比均有非常顯著性差異(P0.01),而E8組中第7級(jí)負(fù)荷與第6級(jí)負(fù)荷相比均具有顯著性差異(P0.05)。(2)血液中LD含量E6組第5級(jí)負(fù)荷與第4級(jí)負(fù)荷相比有非常顯著性差異(P0.01),E8組第6級(jí)負(fù)荷與第5級(jí)負(fù)荷有顯著性差異(P0.05)。(3)血液中SOD的活力E6組、E8組每一級(jí)負(fù)荷與前一級(jí)負(fù)荷相比較都無顯著性差異(P0.05)。2、兩組大鼠在完成同等條件的遞增負(fù)荷運(yùn)動(dòng)時(shí),比目魚肌、紅腓腸肌、白腓腸肌的MDA、H_2O_2含量及SOD活力變化:(1)MDA含量:E6組中比目魚肌、紅腓腸肌、白腓腸肌第6級(jí)負(fù)荷與第5級(jí)負(fù)荷相比均有顯著性差異(P0.01,P0.01,P0.05);在E8組中紅腓腸肌、白腓腸肌第8級(jí)負(fù)荷與第7級(jí)負(fù)荷相比含量均有非常顯著性差異(P0.01),比目魚肌第7級(jí)負(fù)荷與第6級(jí)負(fù)荷相比有顯著性差異(P0.05)。(2)SOD活力:E6組和E8組三種肌肉類型中每級(jí)負(fù)荷與前一級(jí)負(fù)荷相比均無顯著性差異(P0.05)。(3)H_2O_2含量:E6組中比目魚肌、紅腓腸肌第6級(jí)負(fù)荷與第5級(jí)負(fù)荷相比均有顯著性差異(P0.05,P0.01),白腓腸肌第5級(jí)負(fù)荷與第4級(jí)負(fù)荷相比有顯著性差異(P0.01);在E8組中比目魚肌第7級(jí)負(fù)荷與第6級(jí)負(fù)荷相比有顯著性差異(P0.01);紅腓腸肌、白腓腸肌第8級(jí)負(fù)荷與第7級(jí)負(fù)荷相比均有顯著性差異(P0.01,P0.05)。研究結(jié)論:1、在完成相同條件遞增負(fù)荷運(yùn)動(dòng)時(shí),E6組、E8組大鼠血液與肌組織中ROS閾均客觀存在;且血液中ROS閾滯后于血液乳酸閾的出現(xiàn)。2、E8組大鼠血液與肌肉中ROS閾出現(xiàn)要滯后于E6組大鼠。這可能是由于有氧訓(xùn)練增強(qiáng)了線粒體在氧化磷酸化有氧供能水平和提高了抗氧化的能力。3、血液中CK活力突增點(diǎn)出現(xiàn)在血乳酸閾之后,與血液中ROS閾一致。間接說明ROS閾對(duì)應(yīng)的運(yùn)動(dòng)負(fù)荷可能是加劇運(yùn)動(dòng)氧化損傷的臨界負(fù)荷,導(dǎo)致CK從細(xì)胞內(nèi)流出到血液中的活力顯著升高。
[Abstract]:Research purposes: the dynamic changes of reactive oxygen species (ROS) and lactate (LD) in the body are similar to those of the blood, and the concentration changes are closely related to the movement load, which is closely related to the.ROS abrupt point (threshold) indicating the imbalance point between the formation of ROS and the antioxidant clearance, which is of great value for the monitoring of oxidative damage. The objective existence of ROS threshold was verified by comparison between the non training group and the training group, and the phenomenon of whether the ROS threshold in the blood and skeletal muscles in the training group was compared with the untrained group was observed and observed at the same time. At the same time, the LD and the LD in the blood were observed. The dynamic changes of creatine kinase (CK) provide experimental evidence for the in-depth study of the mechanism. Methods: 42 rats of 6 Zhou Lingxiong sex Wistar rats were selected and randomly divided into non exercise training group (group E6, 6 subgroups, 3 in each subgroup, 18); the aerobic exercise training group (group E8, again divided into 8 subgroups, 3 in each subgroup, 24 swimming training for 3 weeks).E After 24 hours of swimming training in the 8 groups, the two groups of rats were given the same condition for one time increasing load exhaustion swimming, and the first level load started from no weight bearing, and the weight of rats in each level was 1%, the 5min.E6 group of each level set up 6 levels, and the group E8 had 8 class load. The rats in each subgroup completed the immediate anesthesia after the corresponding load, taking the blood, taking the ratio. The gastrocnemius, red gastrocnemius, and white gastrocnemius were used to detect the malondialdehyde (MDA), H_2O_2, LD content and CK, SOD activity in blood and muscle tissue. The results were as follows: 1, the changes of MDA, H_2O_2, LD content, SOD and CK activity in the two groups of rats were performed at the same condition of increasing load exercise: (1) MDA in the blood, H_2O_2 content and sixth levels in the vitality group. There were significant differences in the load compared with the fifth level load (P0.01), while the seventh level load in the group E8 had significant differences (P0.05). (2) there was a significant difference in the E6 Group Fifth load in the E6 group of the blood and the fourth grade load (P0.01), and there was a significant difference (P0.05) between the sixth class load and the fifth load (P0.05) in the E8 group. (3) blood. There was no significant difference in the activity of SOD in group E6, and there was no significant difference (P0.05).2 for each level of E8 in group E8. The MDA of soleus, red gastrocnemius, white gastrocnemius, H_2O_2 content and SOD activity in the two groups of rats were completed in the same condition of incremental load exercise: (1) MDA content: the soleus muscle, the red gastrocnemius muscle, and the white gastrocnemius muscle sixth in the E6 group. There were significant differences (P0.01, P0.01, P0.05) in the grade load compared with the fifth stage load. In group E8, the red gastrocnemius muscle, the white gastrocnemius eighth load and the seventh stage load were significantly different (P0.01), compared with the sixth stage load (P0.05). (2) SOD activity: three muscle groups in E6 and E8 groups. There was no significant difference (P0.05) in each stage load in the model (3) H_2O_2 content: there were significant differences between the soleus muscle and the sixth grade load of the red gastrocnemius muscle (P0.05, P0.01) in group E6 (P0.05, P0.01), and the ratio of the white gastrocnemius muscle load to the fourth load (P0.01); in the E8 group, the seventh level load of the soleus muscle was in the E8 group. There was significant difference (P0.01) compared with the sixth level load (P0.01, P0.05) in the red gastrocnemius muscle and the white gastrocnemius eighth load (P0.05). 1. In the completion of the same condition increasing load exercise, the ROS threshold in the blood and the muscle group of the E8 group was all objective, and the ROS threshold in the blood was lagging behind the blood milk. The emergence of acid threshold in.2, ROS threshold in blood and muscle in group E8 rats is lagging behind group E6 rats. This may be due to aerobic training enhanced the mitochondrial oxygen supply level and the ability to increase the antioxidant capacity.3. The sudden increase of CK activity in the blood appears at the threshold of blood lactate, which is consistent with the ROS threshold in the blood. The ROS threshold is indirectly explained. The corresponding exercise load may be aggravated by the critical load of oxidative injury, resulting in a significant increase in the activity of CK from the cells to the blood.

【學(xué)位授予單位】：河北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：G804.2

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