本文選題：恒河猴 + 大腦中動(dòng)脈栓塞　； 參考：《中國比較醫(yī)學(xué)雜志》2017年06期

【摘要】：目的為需要進(jìn)行大腦中動(dòng)脈M1段栓塞模型建立的恒河猴及其它大型實(shí)驗(yàn)動(dòng)物提供一種簡捷、迅速、安全的麻醉方法。方法采用健康成年恒河猴20只,均為雄性,年齡7~9歲,體重7~11 kg,基礎(chǔ)麻醉應(yīng)用0.1 m L/kg氯胺酮及速眠欣的混合液肌內(nèi)注射,待動(dòng)物入睡后行氣管插管(ID:4.5~5.5#)。隨后送入介入中心,入室后連接監(jiān)護(hù)并建立靜脈通路,完成有創(chuàng)動(dòng)脈壓及導(dǎo)尿管置入等。術(shù)中實(shí)驗(yàn)動(dòng)物采用呼吸機(jī)控制呼吸,丙泊酚2~4 mg/kg/h持續(xù)泵入進(jìn)行麻醉維持,術(shù)中根據(jù)動(dòng)物的生命體征和肢體活動(dòng)情況變化調(diào)整劑量,必要時(shí)追加上述氯胺酮與速眠新的混合液,并根據(jù)手術(shù)的需要調(diào)整心率、血壓、體溫等。溶栓前后行腦MRI造影,造影時(shí)停止輸注麻醉藥物,恢復(fù)動(dòng)物自主呼吸。術(shù)中監(jiān)測(cè)實(shí)驗(yàn)動(dòng)物的心率、體溫、有創(chuàng)動(dòng)脈血壓及血氧飽和度,并于麻醉誘導(dǎo)后和術(shù)中抽取動(dòng)脈血行血?dú)夥治觥＝Y(jié)果共進(jìn)行實(shí)驗(yàn)20次,均按預(yù)計(jì)方案實(shí)施完成,術(shù)中未發(fā)生動(dòng)物躁動(dòng)、呼吸抑制、心律失常等嚴(yán)重并發(fā)癥,實(shí)驗(yàn)結(jié)束停藥后,實(shí)驗(yàn)動(dòng)物很快清醒并送回動(dòng)物實(shí)驗(yàn)中心,進(jìn)行后續(xù)處理。20只恒河猴15只存活24 h以上,5只死于溶栓后腦出血和大面積腦梗死面積較大。結(jié)論氣管內(nèi)插管全身麻醉合并復(fù)合麻醉維持為此類比較復(fù)雜的介入及MRI實(shí)驗(yàn)的順利完成提供了一種安全、實(shí)用的麻醉方式。
[Abstract]:Objective to provide a simple, rapid and safe anesthetic method for rhesus monkey and other large laboratory animals which need to be embolized by M1 segment of middle cerebral artery.Methods Twenty healthy adult rhesus monkeys, aged 7 years and 9 years old, weighing 711 kg, were injected intramuscularly with 0.1 m L/kg ketamine and Shumianxin mixture solution. After the animals fell asleep, endotracheal intubation was performed.Then it was put into the intervention center, then connected with the monitoring and established the vein passage, completed the invasive arterial pressure and catheter implantation.During the operation, the experimental animals were controlled by ventilator, the propofol was continuously pumped in for anesthesia maintenance, the dosage was adjusted according to the vital signs of the animals and the changes of limb activity during the operation, and the mixture of ketamine and propofol was added when necessary.And adjust heart rate, blood pressure, body temperature and so on according to the need of operation.Cerebral MRI was performed before and after thrombolysis.The heart rate, body temperature, invasive arterial blood pressure and oxygen saturation of experimental animals were monitored during operation. Arterial blood was extracted for blood gas analysis after anesthesia induction and during operation.Results Twenty times of the experiment were carried out according to the expected plan. There were no serious complications such as restlessness, respiratory depression and arrhythmia during the operation. After the experiment was finished, the experimental animals soon woke up and sent back to the animal experimental center.After treatment, 15 rhesus monkeys survived for more than 24 hours and 5 died of intracerebral hemorrhage and large cerebral infarction after thrombolysis.Conclusion Intracheal intubation combined with general anesthesia combined with anesthesia can provide a safe and practical anesthetic method for the complicated interventional and the successful completion of MRI experiment.
【作者單位】： 首都醫(yī)科大學(xué)宣武醫(yī)院麻醉科;
【基金】：北京市醫(yī)院管理局“登峰計(jì)劃”專項(xiàng)經(jīng)費(fèi)資助(編號(hào)DFL20150802) 國家自然科學(xué)基金項(xiàng)目(編號(hào)81500996) 中國博士后科學(xué)基金資助項(xiàng)目(編號(hào)2016T90114)
【分類號(hào)】：R-332;R614

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