本文選題：鹽負(fù)荷　切入點(diǎn)：堿負(fù)荷　出處：《中國(guó)醫(yī)科大學(xué)學(xué)報(bào)》2017年10期

【摘要】：目的探討鹽負(fù)荷和堿負(fù)荷對(duì)孕晚期大鼠離體子宮收縮力和催產(chǎn)素受體(OXTR)水平的影響。方法將42只孕晚期大鼠隨機(jī)均分為3組,A組飲用自來(lái)水,B組飲用2%Na Cl溶液,C組飲用5%Na HCO3溶液;分別于0、12、24、36、48、60和72 h時(shí)從3組中各隨機(jī)選取2只大鼠麻醉,先剖腹采腹主動(dòng)脈血行動(dòng)脈血?dú)夥治?再將子宮縱向剖取數(shù)個(gè)均等肌條,檢測(cè)其肌張力,最后采用ELISA法測(cè)定肌條中OXTR的含量。結(jié)果 3組在0 h時(shí)血Na+、HCO3-濃度差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);在其余各時(shí)間點(diǎn),B組和C組血Na+濃度均顯著高于A組(P0.05),C組血HCO3-濃度顯著高于A組和B組(P0.05)。3組在0 h時(shí)子宮肌條收縮的振幅和頻率差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);B組在其余各時(shí)間點(diǎn)的振幅和頻率(除72 h振幅外)均顯著高于A組(P0.05);C組在12、24和60 h時(shí)振幅顯著高于A組(P0.05),在48、60和72 h時(shí)頻率顯著高于A組(P0.05)。3組0 h時(shí)OXTR含量差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);其余各時(shí)間點(diǎn),B組OXTR含量顯著高于A組(P0.05);C組在12、24、60和72 h時(shí)OXTR含量顯著高于A組(P0.05)。結(jié)論鹽負(fù)荷可使子宮收縮振幅、頻率及子宮表面OXTR表達(dá)顯著增強(qiáng);堿負(fù)荷60 h時(shí)可使子宮收縮振幅、頻率及OXTR表達(dá)三者均顯著增強(qiáng)。
[Abstract]:Objective to investigate the effects of salt and alkali loading on uterine contractility and oxytocin receptor (oxytocin receptor) level in vitro of late pregnant rats.Methods 42 late pregnant rats were randomly divided into three groups: group A drinking tap water, group B drinking 2%Na Cl solution and group C drinking 5%Na HCO3 solution.The arterial blood gas of abdominal aorta was analyzed by laparotomy, and then several equal muscle strips were dissected longitudinally, and the muscle tension was detected. Finally, the content of OXTR in muscle strips was determined by ELISA method.Results at 0 h, there was no significant difference in serum Na ~ (2 +) -HCO ~ (3-) concentration among the three groups, and at other time points, the serum Na concentration of group B and group C was significantly higher than that of group A (P 0.05) and group B (P 0.05c) at 0 h, and that of group B was significantly higher than that of group A and group B (P 0.05.3) at 0 h.There was no significant difference in the amplitude and frequency of muscle strip contraction. The amplitude and frequency of group B (except 72 h amplitude) at other time points were significantly higher than those of group A at 1224 h and 60 h, respectively, and the amplitudes of group B were significantly higher than those of group A at 24 h and 60 h.The frequency at h was significantly higher than that in group A at 0 h, and the content of OXTR in group B was significantly higher than that in group A at 12: 24 and 72 h, and the content of OXTR in group B was significantly higher than that in group A at 0 h.Conclusion the amplitude and frequency of uterine contraction and the expression of OXTR on uterine surface were significantly increased by salt loading, and the amplitude, frequency and OXTR expression of uterine contraction were significantly increased at 60 h of alkali loading.
【作者單位】： 錦州醫(yī)科大學(xué)中國(guó)人民解放軍第202醫(yī)院研究生培養(yǎng)基地;中國(guó)人民解放軍第202醫(yī)院婦產(chǎn)科;
【基金】：遼寧省科學(xué)技術(shù)計(jì)劃(2014225005)
【分類(lèi)號(hào)】：R714

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