本文關(guān)鍵詞： 氯胺酮 學(xué)習(xí)記憶能力 小膠質(zhì)細(xì)胞 kDa轉(zhuǎn)位蛋白　出處：《山東大學(xué)學(xué)報(醫(yī)學(xué)版)》2015年04期 　論文類型：期刊論文

【摘要】：目的觀察幼年小鼠連續(xù)多次接受氯胺酮麻醉后學(xué)習(xí)記憶認(rèn)知功能及海馬腦區(qū)內(nèi)TSPO蛋白的表達(dá)情況。方法出生21 d的幼年小鼠24只隨機(jī)分為對照組(n=12)和氯胺酮組(n=12),每天1次分別經(jīng)腹腔注射生理鹽水和氯胺酮,連續(xù)7 d。然后進(jìn)行Morris水迷宮實驗,包括4 d的適應(yīng)性訓(xùn)練和1 d的空間探索實驗,觀察小鼠的學(xué)習(xí)記憶功能的改變。水迷宮行為學(xué)測試后處死小鼠,取雙側(cè)的海馬腦區(qū),采用Western blotting技術(shù)和免疫熒光雙標(biāo)技術(shù)檢測小鼠海馬內(nèi)小膠質(zhì)細(xì)胞的變化和TSPO表達(dá)水平。結(jié)果訓(xùn)練期3~4 d,氯胺酮組小鼠潛伏期明顯長于對照組(P0.05)。探索實驗顯示,與對照組比較,氯胺酮組小鼠在平臺所在象限滯留時間和經(jīng)過次數(shù)明顯減少(P0.05);Western blotting分析結(jié)果表明,氯胺酮組幼年小鼠海馬小膠質(zhì)細(xì)胞標(biāo)記物Iba-1和TSPO蛋白表達(dá)明顯增多;免疫熒光雙重標(biāo)記技術(shù)顯示,氯胺酮組小鼠海馬小膠質(zhì)細(xì)胞發(fā)生了形態(tài)學(xué)的改變,TSPO蛋白的表達(dá)增強(qiáng)。結(jié)論氯胺酮組小鼠海馬腦區(qū)內(nèi)小膠質(zhì)細(xì)胞被激活,與之共同表達(dá)的TSPO蛋白的表達(dá)增高,學(xué)習(xí)記憶等認(rèn)知功能減退。
[Abstract]:Objective to observe the cognitive function of learning and memory and the expression of TSPO protein in hippocampus of young mice after repeated ketamine anesthesia. Methods Twenty-four young mice were randomly divided into control group (n = 24). N) and ketamine group (n = 12). Saline and ketamine were injected intraperitoneally once a day for 7 days, then Morris water maze test was performed, including adaptability training for 4 days and space exploration experiment for 1 day. The changes of learning and memory function in mice were observed. The mice were killed after water maze behavior test and bilateral hippocampal regions were taken. Western blotting technique and immunofluorescence double labeling technique were used to detect the changes of microglia and the expression of TSPO in the hippocampus of mice. The incubation period of ketamine group was longer than that of control group (P 0.05). The retention time and pass times of ketamine group in platform quadrant decreased significantly (P 0.05). The results of Western blotting analysis showed that the expression of Iba-1 and TSPO protein in hippocampal microglia of young mice in ketamine group was significantly increased. Immunofluorescence double labeling technique showed that there were morphological changes in hippocampal microglia in ketamine group and the expression of TSPO protein was enhanced. Conclusion Ketamine group can activate microglia in hippocampal area of mice. The expression of TSPO protein was increased and cognitive function such as learning and memory was decreased.
【作者單位】： 濰坊醫(yī)學(xué)院麻醉學(xué)系;解放軍第八十九醫(yī)院麻醉科;
【基金】：山東省教育廳課題(J13LL02)
【分類號】：R726.1
【正文快照】： 網(wǎng)絡(luò)出版地址:http://www.cnki.net/kcms/detail/37.1390.R.20150309.1025.001.html術(shù)后認(rèn)知功能障礙(postoperative cognitive dys-function,POCD)是麻醉手術(shù)后中樞神經(jīng)系統(tǒng)常見的并發(fā)癥之一,學(xué)習(xí)和記憶是認(rèn)知功能的主要組成部分。研究顯示,麻醉藥可能是導(dǎo)致認(rèn)知功能障礙的重

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