甲狀旁腺素相關(guān)肽核定位序列和C-末端缺失對小鼠下頜骨發(fā)育的影響
發(fā)布時間：2019-06-21 10:46
【摘要】：目的:研究甲狀旁腺素相關(guān)肽(parathyroid hormone relative peptide,PTHrP)核定位序列和C-末端缺失對小鼠下頜骨發(fā)育的影響。方法:使用CT掃描三維重建、HE染色、總膠原染色、堿性磷酸酶(alkaline phosphatase,ALP)染色、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色、免疫組織化學(xué)染色和western-blot等方法對PTHrP敲入(PTHrP knock in,PTHrP KI)小鼠(PTHrP KI組,n=10)和野生型(wild type,WT)小鼠的下頜第一磨牙的差異。結(jié)果:WT小鼠成骨細胞密度、下頜骨相對骨量、ALP陽性面積、一型膠原(collagen-I)陽性面積均明顯高于PTHrP KI小鼠,并且差異均具有統(tǒng)計學(xué)意義(P0.05);而PTHrP KI小鼠下頜骨p16,p21和p27蛋白表達水平明顯高于WT小鼠,Ki-67蛋白表達水平低于WT小鼠,并且差異均具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:PTHrP核定位序列和C-末端缺失導(dǎo)致了成骨細胞減少,抑制了胞外基質(zhì)的合成和礦化,從而導(dǎo)致了小鼠下頜骨的發(fā)育障礙。
[Abstract]:Objective: To study the effects of the nuclear localization sequence and C-terminal deletion of parathyroid hormone-related peptide (PTHrP) on the mandibular development of mice. Methods: The PTHrP knock in (PTHrP KI) mice (PTHrP knock in, PTHrP KI) (PTHrP KI) mice (PTHrP knock in, PTHrP KI) (PTHrP KI) were detected by CT scanning three-dimensional reconstruction, HE staining, total collagen staining, alkaline phosphatase (ALP) staining, anti-tartrate acid phosphatase (TRAP) staining, immunohistochemical staining and western-blot. N = 10) and the difference in the mandibular first molar of the wild type (WT) mice. Results: The density of osteoblasts in WT mice, the relative bone mass of the mandible, the positive area of ALP, the positive area of collagen-I were higher than that of the PTHrP KI mice, and the difference was statistically significant (P0.05). The expression of p21 and p27 protein was significantly higher than that of WT mice, and the expression of Ki-67 protein was lower than that of WT mice, and the difference was statistically significant (P0.05). Conclusion: The localization of PTHrP and the deletion of C-terminal lead to the decrease of the osteoblast and the inhibition of the synthesis and mineralization of the extracellular matrix, which leads to the development of the mandible of the mouse.
【作者單位】： 江蘇醫(yī)藥職業(yè)學(xué)院;
【基金】：鹽城市醫(yī)學(xué)科技發(fā)展計劃項目(編號:YK2016049)
【分類號】：R782
本文編號：2504017
[Abstract]:Objective: To study the effects of the nuclear localization sequence and C-terminal deletion of parathyroid hormone-related peptide (PTHrP) on the mandibular development of mice. Methods: The PTHrP knock in (PTHrP KI) mice (PTHrP knock in, PTHrP KI) (PTHrP KI) mice (PTHrP knock in, PTHrP KI) (PTHrP KI) were detected by CT scanning three-dimensional reconstruction, HE staining, total collagen staining, alkaline phosphatase (ALP) staining, anti-tartrate acid phosphatase (TRAP) staining, immunohistochemical staining and western-blot. N = 10) and the difference in the mandibular first molar of the wild type (WT) mice. Results: The density of osteoblasts in WT mice, the relative bone mass of the mandible, the positive area of ALP, the positive area of collagen-I were higher than that of the PTHrP KI mice, and the difference was statistically significant (P0.05). The expression of p21 and p27 protein was significantly higher than that of WT mice, and the expression of Ki-67 protein was lower than that of WT mice, and the difference was statistically significant (P0.05). Conclusion: The localization of PTHrP and the deletion of C-terminal lead to the decrease of the osteoblast and the inhibition of the synthesis and mineralization of the extracellular matrix, which leads to the development of the mandible of the mouse.
【作者單位】： 江蘇醫(yī)藥職業(yè)學(xué)院;
【基金】：鹽城市醫(yī)學(xué)科技發(fā)展計劃項目(編號:YK2016049)
【分類號】：R782
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