發(fā)布時(shí)間：2019-04-07 18:24

【摘要】：目的：研究反復(fù)熔鑄對(duì)鈷鉻、純鈦、鈀基和金鉑等四種非鎳基烤瓷合金溶血性能及口腔粘膜刺激性的影響。 方法：將上述4種烤瓷合金于真空加氬氣下分別熔鑄1~3次，得到1~3代測(cè)試試件。再次熔鑄前，將廢舊合金按“非鎳基烤瓷合金再次熔鑄前處理工藝”處理。（1）根據(jù)ISO/TR7405-1984標(biāo)準(zhǔn)，將4種烤瓷合金熔鑄后，制成圓片狀測(cè)試試件并打磨拋光，消毒滅菌后制備烤瓷合金浸提液。將試件浸提液依次加入生理鹽水、新鮮抗凝兔血，離心，取上清液于分光光度計(jì)測(cè)試吸光度值，計(jì)算溶血率，用SPSS17.0統(tǒng)計(jì)軟件對(duì)同種烤瓷合金3代間的溶血吸光度值進(jìn)行方差分析。（2）根據(jù)YY-T0127.1-1993標(biāo)準(zhǔn)，將4種烤瓷合金經(jīng)1~3次熔鑄后，制成圓片狀測(cè)試試件并打磨拋光，以聚丙烯為陰性對(duì)照材料，工業(yè)聚氯乙烯為陽性對(duì)照材料，制成相同大小的圓片狀試件。將試件縫合固定于金黃地鼠的一側(cè)頰囊粘膜，14天后觀察與試件接觸的頰囊粘膜情況并評(píng)分，同時(shí)切取該粘膜組織制作HE染色切片并進(jìn)行病理評(píng)分。 結(jié)果：（1）4種非鎳基烤瓷合金經(jīng)1~3次熔鑄后，其溶血率均小于5%，同種烤瓷合金1~3代間溶血吸光度值的差異無統(tǒng)計(jì)學(xué)意義（P0.05）。（2）通過14天的觀察發(fā)現(xiàn),金黃地鼠活動(dòng)、飲食正常；試驗(yàn)組局部粘膜與陰性對(duì)照組無明顯差異，未見充血、出血、糜爛、潰瘍等，陽性對(duì)照組粘膜表面出現(xiàn)了較明顯的充血、紅腫。顯微鏡下觀察，試驗(yàn)組上皮完整、連續(xù)，細(xì)胞層次清晰，基底層細(xì)胞形態(tài)規(guī)則，呈矮柱狀，無或少量炎癥細(xì)胞浸潤，組織輕度水腫，，與陰性對(duì)照組無顯著差異。除了鈷鉻3代烤瓷合金對(duì)口腔粘膜的刺激性為輕度外，4種非鎳基烤瓷合金經(jīng)1~3次熔鑄后，其對(duì)口腔粘膜的刺激為極輕度或無刺激。 結(jié)論：鈷鉻、純鈦、鈀基與金鉑等4種非鎳基烤瓷合金經(jīng)過1～3次熔鑄后，其溶血性能及口腔粘膜刺激性符合相關(guān)要求。
[Abstract]:Aim: to study the effects of repeated melting casting on hemolytic properties and oral mucosal irritation of four non-nickel-based porcelain alloys, such as cobalt-chromium, pure titanium, palladium-based and gold-platinum. Methods: the above four kinds of ceramic alloys were fused and cast in vacuum and argon for 1 ~ 3 times respectively, and the test specimens of 1 ~ 3 generations were obtained. Before re-casting, the waste alloys were treated according to the "pre-casting process for non-nickel-based porcelain alloys". (1) according to the ISO/TR7405-1984 standard, four kinds of ceramic alloys were fused and cast, and then made into wafer-shaped test specimens and polished. The ceramic alloy extract solution was prepared after disinfection and sterilization. The sample extract was added saline in turn, fresh anticoagulant rabbit blood was centrifuged, the supernatant was taken to test the absorbance value by spectrophotometer, and the hemolysis rate was calculated. The SPSS17.0 statistical software was used to analyze the variance of the hemolytic absorbance value of the same porcelain-to-porcelain alloy in the third generation. (2) according to the YY-T0127.1-1993 standard, four kinds of porcelain-fused-to-porcelain alloys were melted and cast for 3 times, and then the test specimens were made and polished. Using polypropylene as negative control material and industrial polyvinyl chloride as positive control material, circular specimen of the same size was made. The specimens were sutured to one side of the hamster buccal pouch mucosa. 14 days later, the buccal pouch mucosa in contact with the specimen was observed and scored. At the same time, the mucosa tissue was cut to make HE staining section and the pathological score was carried out. Results: (1) the hemolysis rate of 4 kinds of non-nickel-based porcelain alloys were all less than 5% after 3 times of melting and casting. There was no significant difference in hemolytic absorbance between the same porcelain-fused-to-metal alloys (P0.05). (2) after 14 days of observation, and there was no significant difference in the hemolytic absorbance between the first generation and the third generation of the same ceramic alloy (P0.05). Golden hamster activity, normal diet; There was no significant difference between the experimental group and the negative control group, but no congestion, bleeding, erosion, ulcer and so on appeared on the surface of the mucosa of the positive control group, and there was obvious hyperemia and redness on the surface of the mucous membrane in the positive control group. Under the microscope, the epithelium of the experimental group was intact and continuous, the cell level was clear, the basal cell morphology was regular, with low columnar shape, no or a few inflammatory cells infiltration, slight edema of the tissue, and there was no significant difference between the test group and the negative control group. Except for the mild irritation of Co-Cr 3 generation porcelain alloy to oral mucosa, the stimulation of 4 kinds of non-nickel-based porcelain-ceramic alloys on oral mucosa was very mild or non-irritating after 3 times melting and casting of 4 kinds of non-nickel-based porcelain alloys. Conclusion: the hemolytic properties and oral mucosal irritation of four kinds of non-nickel-based porcelain alloys, such as cobalt-chromium, pure titanium, palladium-based and gold-platinum, meet the relevant requirements after 1 ~ 3 times of melting and casting.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李榮昌，柳梅;金屬離子對(duì)人紅細(xì)胞膜的作用與金屬離子性質(zhì)的關(guān)系[J];北京醫(yī)科大學(xué)學(xué)報(bào);1995年03期

2 洪巖松;李秀梅;孟賀;戰(zhàn)德松;艾紅軍;;幾種齒科合金生物相容性的比較[J];材料研究學(xué)報(bào);2012年02期

3 趙偉;程輝;鄭志強(qiáng);郭永錦;李秀容;;反復(fù)熔鑄對(duì)非貴金屬烤瓷合金鑄流率的影響[J];福建醫(yī)科大學(xué)學(xué)報(bào);2008年03期

4 施巧蕊;童新文;卞麗萍;梁鳴;李鎮(zhèn)祥;鄭明;程輝;;反復(fù)熔鑄對(duì)鈷鉻烤瓷合金抗腐蝕性能影響的研究[J];福州大學(xué)學(xué)報(bào)(自然科學(xué)版);2012年01期

5 郭慶泰,郭劍,馬晶,王昌生;口腔修復(fù)鑄造體投金量的統(tǒng)計(jì)分析[J];口腔材料器械雜志;1999年04期

6 翁蓓軍,鄧漢龍;不同金屬基底材料烤瓷冠對(duì)牙齦組織影響的臨床比較[J];口腔材料器械雜志;2005年02期

7 黃鷙英;745件金屬烤瓷修復(fù)體的臨床分析[J];口腔頜面修復(fù)學(xué)雜志;2002年03期

8 胡欣;魏強(qiáng);李長義;鄧嘉胤;劉霜;朱東望;張連云;;新型口腔修復(fù)用鈦鋯鈮錫合金的生物安全性評(píng)價(jià)[J];口腔頜面修復(fù)學(xué)雜志;2010年01期

9 袁堂霞;張彥表;吳正華;柳云霞;;純鈦烤瓷全冠對(duì)基牙牙周組織健康的影響[J];口腔頜面修復(fù)學(xué)雜志;2011年02期

10 洪巖松;賈迎杰;戰(zhàn)德松;;自制烤瓷合金的生物相容性評(píng)價(jià)[J];口腔醫(yī)學(xué);2008年11期

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