根管-根尖周復合體體外模型的建立
發(fā)布時間：2019-02-16 18:40
【摘要】：目的建立根管-根尖周組織復合體體外模型,以此探索感染根管形成根尖周炎及根尖生物膜的可能機制。方法將因正畸減數(shù)拔除的健康單根前磨牙密封于盛有LB固體培養(yǎng)基的無菌小瓶內(nèi),使牙根的根尖1/3置于培養(yǎng)基中,制備成根管-根尖周復合體體外模型25個。建模后1d抽取5個模型通過PCR技術檢測根尖周組織有無細菌。將余下的20個模型分為對照組(n=10)及實驗組(n=10),實驗組作開髓處理,對照組不做任何處理,自然放置于空氣中,經(jīng)21d檢測根管內(nèi)和根尖周有無細菌,及終點顯色法檢測根尖周內(nèi)毒素濃度。結(jié)果第21天,實驗組根管內(nèi)檢測到細菌,對照組根管內(nèi)未檢測到細菌,而所有模型根尖周均檢測到細菌;對照組根尖周內(nèi)毒素含量平均為(8.913±0.614)EU/mL,實驗組為(10.525±0.981)EU/mL,兩組比較差異有統(tǒng)計學意義(P0.01)。結(jié)論通過上述方法成功建立根管-根尖周復合體體外模型。未對根管做處理時,感染根管內(nèi)的細菌不會到達根尖周,而感染根管內(nèi)的細菌首先是通過分泌的內(nèi)毒素等致病因子到達根尖周引起根尖周炎。
[Abstract]:Objective to establish a model of root canal-periapical tissue complex in vitro to explore the possible mechanism of apical periodontitis and apical biofilm caused by infection of root canals. Methods the healthy premolars extracted by orthodontic subtraction were sealed in a sterile flask containing LB solid medium. The root tip of the root was placed in the medium for 25 models of root canal-periapical complex in vitro. On the first day after modeling, 5 models were selected to detect the presence of bacteria in periapical tissue by PCR technique. The remaining 20 models were divided into two groups: control group (n = 10) and experimental group (n = 10). The experimental group was treated with open pulp, and the control group was placed in the air without any treatment. After 21 days, the root canal and the periapical zone were detected for the presence of bacteria in the root canal and periapical zone. The concentration of endotoxin in periapical region was determined by the end point colorimetric method. Results on the 21st day, bacteria were detected in the root canals in the experimental group, but not in the root canals in the control group. The average periapical endotoxin content in the control group was (8.913 鹵0.614) EU/mL, and (10.525 鹵0.981) EU/mL, in the experimental group. There was significant difference between the two groups (P0.01). Conclusion the in vitro model of root canal-periapical complex was successfully established by the above methods. When the root canal is not treated, the bacteria in the infected root canal will not reach the periapical zone, but the bacteria in the infected root canal will first reach the periapical periodontitis by secreting pathogenic factors such as endotoxin.
【作者單位】： 重慶醫(yī)科大學附屬口腔醫(yī)院牙體牙髓科/口腔疾病與生物醫(yī)學重慶市重點實驗室;
【基金】：重慶市衛(wèi)生局醫(yī)學科研重點項目(2012-1-053)
【分類號】：R781.34
[Abstract]:Objective to establish a model of root canal-periapical tissue complex in vitro to explore the possible mechanism of apical periodontitis and apical biofilm caused by infection of root canals. Methods the healthy premolars extracted by orthodontic subtraction were sealed in a sterile flask containing LB solid medium. The root tip of the root was placed in the medium for 25 models of root canal-periapical complex in vitro. On the first day after modeling, 5 models were selected to detect the presence of bacteria in periapical tissue by PCR technique. The remaining 20 models were divided into two groups: control group (n = 10) and experimental group (n = 10). The experimental group was treated with open pulp, and the control group was placed in the air without any treatment. After 21 days, the root canal and the periapical zone were detected for the presence of bacteria in the root canal and periapical zone. The concentration of endotoxin in periapical region was determined by the end point colorimetric method. Results on the 21st day, bacteria were detected in the root canals in the experimental group, but not in the root canals in the control group. The average periapical endotoxin content in the control group was (8.913 鹵0.614) EU/mL, and (10.525 鹵0.981) EU/mL, in the experimental group. There was significant difference between the two groups (P0.01). Conclusion the in vitro model of root canal-periapical complex was successfully established by the above methods. When the root canal is not treated, the bacteria in the infected root canal will not reach the periapical zone, but the bacteria in the infected root canal will first reach the periapical periodontitis by secreting pathogenic factors such as endotoxin.
【作者單位】： 重慶醫(yī)科大學附屬口腔醫(yī)院牙體牙髓科/口腔疾病與生物醫(yī)學重慶市重點實驗室;
【基金】：重慶市衛(wèi)生局醫(yī)學科研重點項目(2012-1-053)
【分類號】：R781.34
【參考文獻】
相關期刊論文 前1條
1 莊沛林;高燕;凌均h，
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