發(fā)布時間：2019-01-01 11:11

【摘要】：目的:研究脂多糖(lipopolysaccharide,LPS)對大鼠牙髓細胞牙本質涎磷蛋白(dentin sialophosphoprotein,DSPP)、骨涎蛋白(bone sialoprotein,BSP)及堿性磷酸酶(alkaline phosphatase,ALP)表達的影響。方法:采用組織塊法獲得大鼠牙髓細胞,體外常規(guī)培養(yǎng)并進行鑒定,以含0.1、1、10、100和10000 ng/mL牙齦卟啉單胞菌(Porphyromonas gingivalis,P.g)的LPS作用牙髓細胞1、3、5 d,用實時定量PCR檢測DSPP、ALP、BSP mRNA表達的變化,采用SPSS17.0軟件包對數據進行統(tǒng)計學分析。結果:鏡下貼壁后的細胞形態(tài)多樣,多呈成纖維樣細胞形態(tài),還有部分多角形細胞,胞質突起。實時定量PCR結果顯示,與對照組相比,1、10 ng/mL LPS組大鼠牙髓細胞DSPP、ALP、BSP的mRNA表達增高,100、10000 ng/mL LPS組DSPP、ALP、BSP的mRNA表達均降低;在1、3、5 d時,1、10、100和10000 ng/mL LPS組mRNA表達逐漸減少。0.1 ng/mL LPS對mRNA的表達無顯著影響。3種因子呈現相似的表達變化趨勢。結論:低劑量P.g LPS能促進牙髓細胞ALP、BSP、DSPP的表達,高劑量時則抑制ALP、BSP、DSPP的表達;隨著培養(yǎng)時間的延長,促進作用逐漸減弱,抑制作用逐漸增強。
[Abstract]:Aim: to study the effects of lipopolysaccharide (lipopolysaccharide,LPS) on the expression of sialoprotein (dentin sialophosphoprotein,DSPP), bone sialoprotein (bone sialoprotein,BSP) and alkaline phosphatase (alkaline phosphatase,ALP) in rat dental pulp cells. Methods: rat dental pulp cells were obtained by tissue mass method. The pulp cells were cultured and identified in vitro. The pulp cells were treated with LPS containing 0.1 ng/mL porphyromonas gingivalis (Porphyromonas gingivalis,P.g) for 5 days. Real-time quantitative PCR was used to detect the expression of DSPP,ALP,BSP mRNA and SPSS17.0 software package was used to analyze the data. Results: the morphologies of the cells attached to the wall under microscope were various, mostly fibroblast-like cells, and some polygonal cells with cytoplasmic processes. The results of real-time quantitative PCR showed that compared with the control group, the mRNA expression of DSPP,ALP,BSP in dental pulp cells was increased in 1T 10 ng/mL LPS group, and the mRNA expression of DSPP,ALP,BSP was decreased in 10010 000 ng/mL LPS group. After 5 days, the expression of mRNA was gradually decreased in 110100 and 10000 ng/mL LPS groups. 0. 1 ng/mL LPS had no significant effect on the expression of mRNA. The three factors showed a similar trend of expression. Conclusion: low dose P.g LPS can promote the expression of ALP,BSP,DSPP in dental pulp cells and inhibit the expression of ALP,BSP,DSPP at high dose.
【作者單位】： 青島大學醫(yī)學院;青島大學附屬醫(yī)院口腔內科;
【基金】：青島市科技支撐計劃項目(10-3-3-3-6-nsh)~~
【分類號】：R781

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