【摘要】：目的 探討犬骨髓間充質(zhì)干細(xì)胞復(fù)合磷酸鈣骨水泥支架材料修復(fù)骨缺損效果。為口腔種植修復(fù)中骨組織缺損的修復(fù)與重建提供新思路與方法。 方法 分離培養(yǎng)犬骨髓間充質(zhì)干細(xì)胞（BMSCs），觀察其成骨誘導(dǎo)作用；將細(xì)胞與磷酸鈣骨水泥支架材料（CPC）復(fù)合用于動(dòng)物實(shí)驗(yàn)；在4只比格犬的下頜骨每側(cè)制造3處大小相同的骨缺損。隨機(jī)將骨缺損分為三組：BMSCs-CPC組（移植復(fù)合種子細(xì)胞的支架材料），CPC組（只移植支架材料），空白組（不做任何處理）。移植后分別于4周、8周各處死兩只犬，大體、X線、組織學(xué)觀察不同時(shí)期各組骨缺損的成骨情況。 結(jié)果 骨髓間充質(zhì)干細(xì)胞成骨誘導(dǎo)14天后茜素紅染色可見鈣化結(jié)節(jié)形成，，堿性磷酸酶染色細(xì)胞胞漿出現(xiàn)深色顆粒，表明骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞分化；術(shù)后4、8周各組骨缺損均有不同程度的骨再生，BMSCs-CPC組、CPC組中骨組織再生情況優(yōu)于空白組。BMSCs-CPC組中支架材料降價(jià)情況，新骨形成數(shù)量，骨組織的成熟程度優(yōu)于CPC組。 結(jié)論 骨髓間充質(zhì)干細(xì)胞是一種具有多向分化潛能和高度增殖能力的干細(xì)胞，可以向成骨細(xì)胞分化。磷酸鈣骨水泥支架材料具有良好的生物相容性，對(duì)于維持骨外形加快成骨過(guò)程起到良好的作用。利用組織工程的原理，BMSCs種子細(xì)胞復(fù)合CPC支架材是一種有效的、促進(jìn)新骨再生的骨缺損修復(fù)方法，有利于頜骨高度及寬度的保存，為后期口腔種植修復(fù)提供了有益條件。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cells (MSCs) combined with calcium phosphate cement scaffold for repairing bone defect. To provide a new idea and method for the repair and reconstruction of bone tissue defect in dental implant repair. Methods (BMSCs), was isolated from cultured canine bone marrow mesenchymal stem cells (BMSCs) to observe its osteogenic induction, and the cells were combined with calcium phosphate cement scaffold (CPC) for animal experiment. Three bone defects of the same size were made on each side of the mandible of 4 Beagle dogs. Bone defects were randomly divided into three groups: BMSCs-CPC group (), CPC group) and blank group (no treatment). Two dogs were killed at 4 and 8 weeks after transplantation. The osteogenesis of bone defects in each group was observed by gross X-ray and histology. Results 14 days after osteogenesis of bone marrow mesenchymal stem cells, calcified nodules were found in alizarin red staining, dark granules appeared in the cytoplasm of cells stained with alkaline phosphatase, which indicated that bone marrow mesenchymal stem cells differentiated into osteoblasts. Bone tissue regeneration in BMSCs-CPC group and CPC group was better than that in blank group, but in BMSCs-CPC group, the reduction of scaffold material, the number of new bone formation and the maturity of bone tissue were better than those in CPC group. Conclusion Bone marrow mesenchymal stem cells are a kind of stem cells with multiple differentiation potential and high proliferative ability, which can differentiate into osteoblasts. Calcium phosphate cement scaffold has good biocompatibility and plays a good role in maintaining bone shape to accelerate osteogenesis. Based on the principle of tissue engineering, BMSCs seed cells combined with CPC scaffold is an effective method for repairing bone defect, which is beneficial to the preservation of the height and width of jaw bone, and provides a beneficial condition for the later stage of oral implant repair.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 金瓊;王曉敏;王曉飛;李旭東;麻健豐;;膠原-羥磷灰石復(fù)合膜引導(dǎo)骨組織再生的動(dòng)物實(shí)驗(yàn)研究[J];華西口腔醫(yī)學(xué)雜志;2011年01期

2 汪衛(wèi)國(guó);高杰;姚斌;王篤權(quán);;構(gòu)建過(guò)表達(dá)hBMP-7基因重組腺病毒及體外轉(zhuǎn)染犬骨髓基質(zhì)細(xì)胞的研究[J];口腔醫(yī)學(xué)研究;2009年04期

3 楊麗;張榮華;謝厚杰;蔡宇;黃豐;;建立大鼠骨髓間充質(zhì)干細(xì)胞穩(wěn)定分離培養(yǎng)體系與鑒定[J];中國(guó)組織工程研究與臨床康復(fù);2009年06期

4 蔡黔;董方;劉毅;;異體骨髓間充質(zhì)干細(xì)胞治療大鼠糖尿病足潰瘍及血管內(nèi)皮生長(zhǎng)因子的表達(dá)[J];中國(guó)組織工程研究與臨床康復(fù);2010年36期

5 卜令學(xué);高振華;荊恒;陳立強(qiáng);劉鳳芝;李寧毅;;犬骨髓基質(zhì)干細(xì)胞向成骨細(xì)胞誘導(dǎo)分化及細(xì)胞片層的制備[J];現(xiàn)代口腔醫(yī)學(xué)雜志;2010年05期

6 尹宏宇;劉廣鵬;崔磊;曹誼林;呂長(zhǎng)勝;;Percoll法分離犬骨髓單個(gè)核細(xì)胞與體外成骨誘導(dǎo)培養(yǎng)的實(shí)驗(yàn)研究[J];組織工程與重建外科雜志;2007年05期

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