【摘要】：目的:對比使用軟骨上清液和轉(zhuǎn)化生長因子兩種方法誘導滑膜間充質(zhì)干細胞向軟骨細胞分化。方法:分別采用消化法獲取SD大鼠滑膜間充質(zhì)干細胞和軟骨細胞。體外擴增。實驗組一:通過軟骨細胞上清液誘導滑膜間充質(zhì)干細胞向軟骨方向分化。實驗組二:通過軟骨誘導液(TGF-β1,ITS+Premix,2-磷酸抗壞血酸等)誘導滑膜間充質(zhì)干細胞向軟骨方向分化。培養(yǎng)21d后通過形態(tài)學、免疫組織化學法檢測其生物學特性,RT-PCR檢測誘導后產(chǎn)物Ⅱ型膠原RNA含量。結(jié)果:2種誘導方法均能誘導滑膜間充質(zhì)干細胞成軟骨細胞方向分化。在形態(tài)學可見2組誘導后產(chǎn)物成軟骨樣結(jié)構(gòu),呈乳白色,質(zhì)地韌。免疫組織化學鑒定基質(zhì)能被Ⅱ型膠原染色,細胞染色呈現(xiàn)棕黃色。RT-PCR結(jié)果顯示誘導后的微團表達軟骨特異性基因Ⅱ型膠原和蛋白聚糖。結(jié)論:兩組實驗組均能誘導滑膜間充質(zhì)干細胞向軟骨方向分化。對比兩種實驗方法,使用上清液誘導滑膜間充質(zhì)干細胞向軟骨方向分化能力更強。
[Abstract]:Aim: to induce the differentiation of synovial mesenchymal stem cells into chondrocytes by using cartilage supernatant and transforming growth factor. Methods: SD rat synovial mesenchymal stem cells and chondrocytes were obtained by digestion. In vitro amplification. Experimental group 1: synovial mesenchymal stem cells were induced to differentiate into cartilage by chondrocyte supernatant. Experiment 2: chondrogenic differentiation of synovial mesenchymal stem cells into cartilage was induced by chondrocyte inducing fluid (TGF- 尾 1 its Premix,2- ascorbic acid). After 21 days of culture, the biological characteristics were detected by morphological and immunohistochemical methods, and the content of type 鈪，

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