【摘要】：目的:通過檢測口腔扁平苔蘚(oral lichen planus,OLP)患者外周血T細胞B7-H1及PD-1的表達情況及與疾病狀態(tài)的關(guān)系,以此來探討B(tài)7-H1/PD-1信號通道在OLP患者T細胞免疫中的作用。方法:選擇22例OLP患者,12例健康成人作為對照。應用REU計分系統(tǒng)及視覺模擬評分法(VAS)對OLP患者的疾病狀態(tài)進行計分。密度梯度離心法分離OLP組和對照組外周血中的單個核細胞,流式細胞術(shù)檢測單個核細胞中T細胞表面B7-H1及PD-1的表達水平。貼壁法去除單核細胞后,將OLP患者的淋巴細胞與健康成人淋巴細胞混合培養(yǎng),加入抗B7-H1單克隆抗體阻斷B7-H1/PD-1信號通道,培養(yǎng)5d后應用XTT方法檢測細胞增殖情況。結(jié)果:CD3+B7-H1+、CD3+PD-1+的表達百分率在OLP和正常對照組之間存在有明顯差異(P0.05)。CD3+B7-H1+在OLP組的表達水平與疾病狀態(tài)計分正相關(guān)(P=0.002),而CD3+PD-1+的表達水平與疾病的臨床狀態(tài)計分無關(guān)(P=0.537)。在混合淋巴細胞培養(yǎng)中,使用抗B7-H1單克隆抗體阻斷B7-H1/PD-1信號通道后,淋巴細胞增殖情況與對照組無明顯差異(P0.05)。結(jié)論:OLP患者外周血T細胞中的CD3+B7-H1+、CD3+PD-1+很可能參與了OLP的發(fā)病;CD3+B7-H1+對OLP的疾病進展可能產(chǎn)生一定作用。
[Abstract]:Objective: to detect the expression of B7-H1 and PD-1 in peripheral blood T cells of patients with oral lichen planus (oral lichen planus,OLP) and its relationship with disease status. To explore the role of B7-H1/PD-1 signal channel in T cell immunity of OLP patients. Methods: 22 patients with OLP and 12 healthy adults were selected as control group. REU scoring system and visual analogue score (VAS) were used to score the disease status of OLP patients. Mononuclear cells were isolated from peripheral blood of OLP group and control group by density gradient centrifugation. The expression levels of B7-H1 and PD-1 on T cell surface were detected by flow cytometry. After the monocytes were removed by adherent method, lymphocytes from OLP patients were mixed with healthy adult lymphocytes, and monoclonal antibodies against B7-H1 were added to block the signal channels of B7-H1/PD-1. After 5 days of culture, the proliferation of cells was detected by XTT method. Results: there was significant difference in the expression of CD3 B7-H1 and CD3 PD-1 between OLP and normal control group (P0.05). The expression level of CD3 B7-H1 in OLP group was positively correlated with the score of disease state (P0. 002). However, the expression of CD3 PD-1 was not correlated with the clinical status score (P0. 537). In mixed lymphocyte culture, there was no significant difference in lymphocyte proliferation between the control group and the control group after blocking the B7-H1/PD-1 signal channel with anti B7-H1 monoclonal antibody (P0.05). Conclusion: CD3 B7-H1 and CD3 PD-1 in peripheral blood T cells of OLP patients may be involved in the pathogenesis of OLP, and CD3 B7-H1 may play a role in the progression of OLP.
【作者單位】： 北京華信醫(yī)院(清華大學第一附屬醫(yī)院)口腔科;北京大學口腔醫(yī)院特診科;
【分類號】：R781.5

【參考文獻】

相關(guān)期刊論文 前7條

1 張弘;張英;;口腔扁平苔蘚組織中STATs家族表達與外周血PD-1、PD-L1表達及免疫功能的相關(guān)性[J];海南醫(yī)學院學報;2016年24期

2 李曉英;羅�？�;謝啟超;;Treg細胞與腫瘤免疫的關(guān)系[J];重慶醫(yī)學;2016年25期

3 張小玲;王國恩;張軍;劉媛媛;劉敏龍;;PD-L1及CD86在膿毒癥小鼠外周血單核細胞中的表達趨勢[J];中國急救醫(yī)學;2015年10期

4 諸葛欣;孫寧;王麗莉;肖廣輝;;程序性死亡受體1缺乏對動脈粥樣硬化性免疫反應的影響[J];中華醫(yī)學雜志;2014年30期

5 劉紅梅;王翎;張光波;陳思文;潘旭東;毛燕青;;共刺激分子B7-H1對小鼠髓源性抑制細胞免疫抑制功能的調(diào)節(jié)作用[J];中華老年醫(yī)學雜志;2014年02期

6 翁志宏;葉翩;張淑玲;;阻斷程序性死亡蛋白-1及其配體信號通路增強慢性乙型肝炎病毒感染小鼠的抗病毒免疫[J];中華肝臟病雜志;2010年04期

7 吳偉;宣璐;李幼華;;口腔扁平苔蘚免疫功能變化觀察及其意義[J];現(xiàn)代實用醫(yī)學;2009年11期

【共引文獻】

相關(guān)期刊論文 前10條

1 任莉;韓杰;徐明明;王雋;;OLP患者外周血T細胞B7-H1及PD-1的表達情況及與疾病狀態(tài)的關(guān)系研究[J];口腔醫(yī)學研究;2017年12期

2 張文杰;何英麗;王濤;董晨明;;膿毒癥新常態(tài)及其治療研究進展[J];中國全科醫(yī)學;2017年35期

3 方溢云;;口腔扁平苔蘚外周血Th1/Th2型細胞因子表達水平的價值研究[J];現(xiàn)代診斷與治療;2017年14期

4 郁文燕;潘克女;徐愛芳;朱勇;;血清可溶性白介素受體-2和白細胞介素-6水平與高血壓患者頸動脈病變及氧化應激水平的關(guān)系[J];中國衛(wèi)生檢驗雜志;2017年12期

5 葛志強;梁歌之;廖程;;LAIR-1與TGF-β在口腔扁平苔蘚患者外周血中表達的相關(guān)性研究[J];中外醫(yī)學研究;2017年17期

6 閆秋菊;;口腔扁平苔蘚患者免疫功能狀況及臨床護理效果觀察[J];國際護理學雜志;2016年16期

7 許彥枝;張楊楊;劉健;劉鐵軍;張梅潔;;化濕行瘀清熱方劑治療的口腔扁平苔蘚患者效果及其唾液白蛋白、分泌型IgA水平觀察[J];山東醫(yī)藥;2016年27期

8 羅亮;舒美蘭;李舒眉;蔡揚;;口腔扁平苔蘚患者血清中可溶性程序性死亡受體1及其配體表達與免疫功能的相關(guān)性[J];中華口腔醫(yī)學雜志;2015年10期

9 陳思文;王翎;蘇楠;張光波;劉紅梅;潘旭東;李潔;;老年小鼠CD11b~+GR-1~+髓源性抑制細胞免疫功能特點及機制研究[J];中華老年醫(yī)學雜志;2015年03期

10 王冬平;姜旺展;蔡揚;鞏翔;王s，

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