發(fā)布時間：2018-11-25 21:22

【摘要】：目的:探討α-catulin基因?qū)θ松圜[狀細胞癌(TSCC)TSCCA細胞增殖和凋亡的影響,闡明α-catulin在TSCCA細胞生物學行為中的作用。方法:取對數(shù)生長期的TSCCA細胞,分為α-catulin-siRNA轉(zhuǎn)染組、空質(zhì)粒轉(zhuǎn)染組和空白對照組。RT-PCR法檢測各組TSCCA細胞中α-catulin mRNA相對表達水平;Western blotting法檢測各組TSCCA細胞中α-catulin蛋白的相對表達水平,MTT法檢測細胞生長抑制率,流式細胞術(shù)檢測各組TSCCA細胞凋亡率。結(jié)果:RT-PCR檢測,與空質(zhì)粒轉(zhuǎn)染組和空白對照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細胞中α-catulin mRNA相對表達水平降低(P0.01),且隨著時間的相對延長,α-catulin mRNA相對表達水平逐漸降低,72h時其相對表達水平低于24和48h時(P0.01)。Western blotting法檢測,與空質(zhì)粒轉(zhuǎn)染組和空白對照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細胞中α-catulin蛋白相對表達水平降低(P0.05)。MTT法檢測,與空質(zhì)粒轉(zhuǎn)染組和空白對照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細胞生長抑制率升高(P0.001)。流式細胞術(shù)檢測,與空質(zhì)粒對照組和空白對照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細胞凋亡率升高(P0.001)。結(jié)論:特異性沉默α-catulin基因的表達可抑制TSCC的TSCCA細胞的增殖,促進其凋亡。
[Abstract]:Aim: to investigate the effect of 偽-catulin gene on proliferation and apoptosis of human tongue squamous cell carcinoma (TSCC) TSCCA) cells and to elucidate the role of 偽-catulin in the biological behavior of TSCCA cells. Methods: TSCCA cells in logarithmic growth stage were divided into 偽 catulin-siRNA transfection group, empty plasmid transfection group and blank control group. RT-PCR assay was used to detect the relative expression of 偽 catulin mRNA in TSCCA cells. The relative expression of 偽-catulin protein in TSCCA cells was detected by Western blotting assay, the cell growth inhibition rate was detected by MTT assay, and the apoptosis rate of TSCCA cells in each group was detected by flow cytometry. Results: compared with the blank plasmid transfection group and the blank control group, the relative expression level of 偽-catulin mRNA in the TSCCA cells of the 偽-catulin-siRNA transfection group was decreased (P0.01), and the relative expression of 偽-catulin mRNA was prolonged with time. The relative expression level of 偽-catulin mRNA decreased gradually, and was lower than that at 24 and 48 h at 72 h (P0.01). Western blotting method), compared with the blank plasmid transfection group and the blank control group. The relative expression level of 偽-catulin protein in TSCCA cells was decreased in 偽-catulin-siRNA transfection group (P0.05). MTT method). Compared with empty plasmid transfection group and blank control group, the growth inhibition rate of TSCCA cells in 偽-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Compared with empty plasmid control group and blank control group, the apoptosis rate of TSCCA cells in 偽-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Conclusion: specifically silencing the expression of 偽-catulin gene can inhibit the proliferation of TSCC TSCCA cells and promote its apoptosis.
【作者單位】： 遼寧醫(yī)學院附屬第一醫(yī)院口腔科;
【基金】：遼寧省科技廳自然科學基金資助課題(2015020333)
【分類號】：R739.86

【參考文獻】

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