【摘要】：目的:研究正畸移動大鼠上后牙過程中,PLAP-1、BMP-2在牙周膜的分布和表達。方法:選擇雄性SD大鼠36只,按1d、3d、5d、7d、14d、21d分為6組,均用50g移動左上第一磨牙,右上第一磨牙為對照。在相應時期處死大鼠后測量牙移動距離,采用HE染色及免疫組織化學SP法,檢測不同時期牙周組織的形態(tài)變化;PLAP-1、BMP-2在牙周膜組織中的表達及研究兩者可能存在的關系。結果:不同時期牙移動的距離不同,差別顯著有統(tǒng)計學意義(P0.05)。HE染色顯示對照組牙周組織變化不明顯;實驗組張力區(qū)有成骨細胞生成,壓力區(qū)破骨細胞生成,隨著時間的延長最后兩區(qū)形態(tài)都基本趨一致。免疫組化法示PLAP-1在牙周膜的表達總是張力區(qū)高于壓力區(qū),在第3天張力區(qū)表達最強,除21d外,其余各組間差異顯著有統(tǒng)計學意義(P0.05)。BMP-2在第5天張力側表達最強,在3d、5d、7d的表達差異顯著有統(tǒng)計學意義(P0.05)。結論:PLAP-1,BMP-2在正畸力作用下參與牙周膜組織改建的表達最高值時期不同。
[Abstract]:Objective: to study the distribution and expression of PLAP-1,BMP-2 in periodontal ligament during orthodontic movement of rat upper posterior teeth. Methods: Thirty-six male SD rats were divided into 6 groups according to 1 d, 3 d, 5 d, 7 d, 14 d and 21 d, all of them were divided into 6 groups. The left first molar was moved 50 g and the right first molar was used as control. After the rats were killed at the corresponding period, the distance of tooth movement was measured, the morphological changes of periodontal tissue were detected by HE staining and immunohistochemical SP method, and the expression of PLAP-1,BMP-2 in periodontal ligament tissue and the possible relationship between them were studied. Results: the distance of tooth movement in different period was different, the difference was statistically significant (P0.05). HE staining showed that the periodontal tissue of the control group had no obvious change, the tension zone of experimental group had osteoblast formation, the pressure zone of osteoclast formation, and the change of osteoclast formation in the pressure area. With the extension of time, the last two regions are basically consistent. Immunohistochemical method showed that the expression of PLAP-1 in periodontal ligament was always higher than that in the pressure area, and the expression of BMP-2 was the strongest in the tension area on the 3rd day, except 21 days, there was significant difference between the other groups (P0.05). The expression of BMP-2 was the strongest in the tension side of the periodontal ligament on the 5th day. There was significant difference in the expression of 7 days after 3 days (P 0.05). Conclusion: the maximum expression of PLAP-1,BMP-2 in periodontal ligament tissue remodeling under orthodontic force is different.
【作者單位】： 西南醫(yī)科大學附屬口腔醫(yī)院口腔內科;
【基金】：瀘州市科技局資助項目(編號:20140101)
【分類號】：R783.5

【相似文獻】

相關期刊論文 前10條

1 毛釗;影響牙周膜細胞生物學功能的相關因素[J];醫(yī)學研究生學報;2003年06期

2 凌均h，

本文編號：2275878