【摘要】：目的通過(guò)檢測(cè)不同濃度的生物活性玻璃浸提液對(duì)小鼠L929細(xì)胞的增殖活性、細(xì)胞周期和骨向分化的作用,為生物活性玻璃在牙周組織再生領(lǐng)域的應(yīng)用提供基礎(chǔ)實(shí)驗(yàn)依據(jù)。方法本實(shí)驗(yàn)研究分為兩部分:1生物活性玻璃對(duì)L929細(xì)胞增殖的影響。制備生物活性玻璃浸提液,以10%小牛血清的RPMI1640完全培養(yǎng)液稀成不同濃度的生物活性玻璃(bioactive glass,BG)浸提液培養(yǎng)L929細(xì)胞24h、48h和72h后,采用四唑鹽(MTT)法和流式細(xì)胞術(shù)(FCM)法檢測(cè)不同濃度的生物活性玻璃浸提液對(duì)L929細(xì)胞增殖活性和細(xì)胞周期的影響。2生物活性玻璃對(duì)L929細(xì)胞骨向分化的影響。采用堿性磷酸酶(ALP)活性測(cè)定的方法,分別在24h、48h和72h檢測(cè)不同濃度的生物活性玻璃浸提液對(duì)體外培養(yǎng)L929細(xì)胞骨向分化的影響。結(jié)果MTT和ALP法測(cè)定結(jié)果均顯示培養(yǎng)L929細(xì)胞24h、48h和72h后,隨時(shí)間延長(zhǎng),0.0625g/L~1.25g/L組的OD值均高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05),以0.0625g/L濃度組的OD值最高(P0.05);2.5g/L組的OD值較對(duì)照組稍低,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);5g/L組與對(duì)照組相比OD值顯著降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。FCM實(shí)驗(yàn)結(jié)果與上述實(shí)驗(yàn)結(jié)果相符,BG浸提液濃度為0.0625g/L時(shí)細(xì)胞增殖指數(shù)(PI)最高,濃度為5g/L時(shí)PI值最低(P0.05)。結(jié)論1生物活性玻璃在一定濃度范圍內(nèi)可促進(jìn)L929成纖維細(xì)胞的增殖及骨向化。2生物活性玻璃在低濃度時(shí)促進(jìn)L929細(xì)胞增殖和骨向分化,高濃度時(shí)抑制L929細(xì)胞的增殖和骨向分化。
[Abstract]:Objective to investigate the effects of different concentrations of bioactive glass extract on the proliferation, cell cycle and bone differentiation of mouse L929 cells, and to provide a basic experimental basis for the application of bioactive glass in the field of periodontal tissue regeneration. Methods the experiment was divided into two parts: 1 the effect of bioactive glass on the proliferation of L 929 cells. The bioactive glass extract was prepared and the L929 cells were incubated with 10% bovine serum RPMI1640 complete culture medium with different concentrations of bioactive glass (bioactive glass,BG) for 48 h and 72 h. The effects of different concentrations of bioactive glass extract on the proliferation activity and cell cycle of L929 cells were detected by (MTT) and flow cytometry. 2 the effects of bioactive glass on bone differentiation of L929 cells were studied. The effects of different concentrations of bioactive glass extract on bone differentiation of L929 cells in vitro were determined by alkaline phosphatase (ALP) assay at 24 h and 72 h, respectively. Results the results of MTT and ALP assay showed that the OD value of 0.0625g/L~1.25g/L group was higher than that of control group (P0.05), the OD value of 0.0625g/L group was the highest (P0.05), the OD value of 2.5g/L group was slightly lower than that of control group. There was no significant difference (P0.05); compared with the control group, the OD value of 5g/L group was significantly lower than that of the control group (P0.05). FCM experimental results were consistent with the above experimental results, the highest (PI) cell proliferation index (PI) when the concentration of BG extract solution was 0.0625g/L, PI value was the lowest when 5g/L concentration was P05). Conclusion (1) Bioactive glass can promote the proliferation and osteotropism of L929 fibroblasts in a certain concentration range. (2) Bioactive glass can promote the proliferation and bone differentiation of L929 cells at low concentration, and inhibit the proliferation and bone differentiation of L929 cells at high concentration.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.42

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