發(fā)布時(shí)間：2018-06-24 10:46

  本文選題：濃縮生長(zhǎng)因子 + 骨結(jié)合　； 參考：《華西口腔醫(yī)學(xué)雜志》2015年01期

【摘要】：目的探討濃縮生長(zhǎng)因子提取液(CGFe)對(duì)鈦片表面MC3T3-E1細(xì)胞增殖分化的影響。方法實(shí)驗(yàn)組使用含CGFe的α-MEM培養(yǎng)液(含10%胎牛血清)培養(yǎng)MC3T3-E1細(xì)胞,對(duì)照組則使用不含CGFe的α-MEM培養(yǎng)液(含10%胎牛血清)培養(yǎng)細(xì)胞。采用MTT法測(cè)定培養(yǎng)1、3、5 d時(shí)的細(xì)胞增殖情況,堿性磷酸酶(ALP)活性測(cè)定法檢測(cè)培養(yǎng)3、5 d時(shí)的細(xì)胞分化情況;通過(guò)掃描電子顯微鏡(SEM)觀察培養(yǎng)12 h時(shí)細(xì)胞在鈦片表面的形態(tài);通過(guò)熒光實(shí)時(shí)定量聚合酶鏈反應(yīng)(PCR)測(cè)定細(xì)胞培養(yǎng)3、7 d時(shí)核心結(jié)合蛋白因子2(Runx2)和成骨細(xì)胞特異性轉(zhuǎn)錄因子Osterix(Osx)基因的相對(duì)表達(dá)量。結(jié)果 MTT結(jié)果顯示:隨培養(yǎng)時(shí)間延長(zhǎng),細(xì)胞數(shù)量逐漸增加;各時(shí)間點(diǎn)實(shí)驗(yàn)組細(xì)胞的吸光度值均明顯高于對(duì)照組(P0.05)。ALP活性隨著培養(yǎng)時(shí)間延長(zhǎng)而增加,兩個(gè)時(shí)間點(diǎn)實(shí)驗(yàn)組的吸光度值均明顯高于對(duì)照組(P0.05)。SEM觀察:培養(yǎng)12 h時(shí),實(shí)驗(yàn)組細(xì)胞在鈦片表面的形態(tài)較對(duì)照組更加伸展。PCR結(jié)果顯示:隨著培養(yǎng)時(shí)間延長(zhǎng),兩組細(xì)胞Runx2和Osx基因的表達(dá)量逐漸增加,兩個(gè)時(shí)間點(diǎn)實(shí)驗(yàn)組的表達(dá)量均明顯高于對(duì)照組(P0.05)。結(jié)論 CGFe能有效地促進(jìn)MC3T3-E1細(xì)胞的增殖、分化及在鈦片表面的伸展。
[Abstract]:Objective to investigate the effect of concentrated growth factor extract (CGFe) on the proliferation and differentiation of MC3T3-E1 cells on titanium sheet. Methods MC3T3-E1 cells were cultured in 偽 -MEM medium (containing 10% fetal bovine serum) in experimental group and 偽 -MEM medium without CGFe (10% fetal bovine serum) in control group. MTT assay was used to determine the proliferation of cells at 5 days after culture, alkaline phosphatase (ALP) activity assay was used to detect the differentiation of cells at 5 days, scanning electron microscopy (SEM) was used to observe the morphology of the cells on the surface of titanium sheet after 12 hours culture. The relative expression of core binding protein factor 2 (Runx2) and osteoblast specific transcription factor Osterix (Osx) were measured by fluorescence real time quantitative polymerase chain reaction (PCR). Results MTT results showed that the number of cells increased gradually with the increase of culture time, and the absorbance value of experimental group was significantly higher than that of control group (P0.05). ALP activity increased with the increase of culture time. The absorbance of the experimental group was significantly higher than that of the control group (P0.05) .SEM observation at two time points: after 12 hours of culture, the morphology of the cells on the surface of the titanium slice in the experimental group was more extensive than that in the control group. The expression of Runx2 and Osx genes in the two groups increased gradually, and the expression levels of the experimental group were significantly higher than those of the control group at two time points (P0.05). Conclusion CGFe can effectively promote the proliferation, differentiation and extension of MC3T3-E1 cells.
【作者單位】： 煙臺(tái)市口腔醫(yī)院修復(fù)科;
【分類號(hào)】：R783

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