本文選題：低氧預處理 + 腺苷酸單磷酸激活蛋白激酶(AMPK)　； 參考：《牙體牙髓牙周病學雜志》2014年07期

【摘要】：目的:觀察低氧預處理以及腺苷酸單磷酸激活蛋白激酶(AMPK)過表達對過氧化氫(H2O2)抑制牙髓細胞增殖的影響。方法:牙髓細胞分別在常氧(950 mL/L O2)和低氧(10 mL/L O2)環(huán)境下培養(yǎng)12 h后,用qRT-PCR檢測細胞中AMPK mRNA的表達水平;分別取常氧和低氧培養(yǎng)12 h的牙髓細胞與0.1 mmol/L H2O2共培養(yǎng)4 h后,常規(guī)條件下繼續(xù)培養(yǎng)4 d,用MTT法檢測細胞的增殖能力;分別用AMPK過表達質粒和特異性小RNA干擾(siRNA)片段轉染牙髓細胞,與0.1 mmol/L H2O2共培養(yǎng)4 h,然后常規(guī)條件下繼續(xù)培養(yǎng)4 d,用MTT法檢測細胞的增殖能力。結果:低氧預處理能顯著上調牙髓細胞中AMPK mRNA的表達水平,并能減輕H2O2對細胞增殖的抑制作用(P0.05);AMPK過表達細胞經過H2O2處理后,增殖能力明顯高于正常細胞+H2O2組和siRNA+H2O2組(P0.05),而siRNA干擾的細胞經H2O2處理后其增殖能力則較其他各組降低(P0.05)。結論:低氧預處理和AMPK基因的上調可使牙髓細胞獲得對H2O2氧化的抗性,從而減輕H2O2對細胞增殖的抑制作用。
[Abstract]:Aim: to investigate the effects of hypoxia preconditioning and overexpression of adenylate monophosphate-activated protein kinase (AMPK) on the proliferation of dental pulp cells inhibited by H _ 2O _ 2 H _ 2O _ 2. Methods: dental pulp cells were cultured in normoxic (950 mL / L) and hypoxia (10 mL / L) for 12 h. The expression of AMPK mRNA in dental pulp cells was detected by qRT-PCR and co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h in normoxic and hypoxic culture for 12 h. The proliferation of dental pulp cells was detected by MTT assay and transfected into dental pulp cells by AMPK overexpression plasmids and specific small RNA interference siRNAs. The cells were co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h, then cultured for 4 days under conventional conditions. The proliferation of the cells was measured by MTT assay. Results: hypoxia pretreatment could significantly up-regulate the expression of AMPK mRNA in dental pulp cells and reduce the inhibitory effect of H _ 2O _ 2 on cell proliferation. The proliferative ability of siRNA interference cells was significantly higher than that of normal cells treated with H 2O 2 and siRNA H 2O 2 groups, but the proliferation ability of siRNA interference cells treated with H 2O 2 was lower than that of other groups. Conclusion: hypoxia preconditioning and up-regulation of AMPK gene can make dental pulp cells become resistant to H2O2 oxidation, thus reducing the inhibitory effect of H2O2 on cell proliferation.
【作者單位】： 安康市中心醫(yī)院口腔科;神木縣醫(yī)院口腔科;
【分類號】：R780.2
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本文編號：2043285