本文選題：十肽 + 抗菌性能　； 參考：《華西口腔醫(yī)學(xué)雜志》2014年06期

【摘要】：目的評(píng)價(jià)人工合成抗菌肽(十肽)對(duì)口腔常見感染性疾病主要致病菌的抑菌活性。方法采用瓊脂擴(kuò)散法及液體稀釋法體外評(píng)價(jià)十肽對(duì)變異鏈球菌、表兄鏈球菌、嗜酸乳桿菌、血鏈球菌、格氏鏈球菌、黏性放線菌、內(nèi)氏放線菌、牙齦卟啉單胞菌、中間普雷沃菌、具核梭桿菌、伴放線嗜血桿菌及白色假絲酵母菌的抑菌性能,并測定十肽對(duì)變異鏈球菌的時(shí)間-殺菌曲線。結(jié)果十肽對(duì)所選實(shí)驗(yàn)菌株均表現(xiàn)出不同的抑菌性能,對(duì)主要致齲菌的最小抑菌濃度MIC值為62.5~125μg·m L-1,而對(duì)主要牙周致病菌的MIC值為250~1 000μg·m L-1,其中,十肽對(duì)齲病主要致病菌變異鏈球菌有較強(qiáng)抑菌作用。時(shí)間-殺菌曲線結(jié)果顯示,十肽作用20 min后開始?xì)⒕?30 min之后可完全殺滅細(xì)菌,且在24 h之內(nèi)無細(xì)菌生長。結(jié)論新型人工合成抗菌肽十肽對(duì)口腔常見感染性疾病主要致病菌具有抑菌性能,其中對(duì)致齲變異鏈球菌的抑菌效果最為明顯。
[Abstract]:Objective to evaluate the antibacterial activity of synthetic antimicrobial peptides (CTPs) against the main pathogens of common oral infectious diseases. Methods Agar diffusion method and liquid dilution method were used to evaluate in vitro the effects of decapeptide on Streptococcus mutans, Streptococcus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gravis, Actinomyces viscosity, Actinomyces Neisseri and Porphyromonas gingivalis. The bacteriostatic properties of Prevobacter intermedia, Clostridium nucleatum, Haemophilus actinophilus and Candida albicans were determined, and the time-bactericidal curves of decapeptide against Streptococcus mutans were determined. Results the decapeptide showed different bacteriostatic properties against the selected strains. The minimum inhibitory concentration (MIC) was 62.5 ~ 125 渭 g mL ~ (-1) for the main cariogenic bacteria, while the MIC value for the main periodontal pathogenic bacteria was 250 ~ 1 000 渭 g / L ~ (-1). Decapeptide has strong bacteriostatic effect on Streptococcus mutans, the main pathogen of caries. The results of time-bactericidal curve showed that the decapeptide could kill the bacteria completely after 20 min treatment and no bacteria growth within 24 h after sterilization for 30 min. Conclusion the new synthetic antimicrobial peptide decapeptide has antimicrobial activity against the main pathogenic bacteria of common oral infectious diseases, among which the bacteriostasis effect is the most obvious for Streptococcus mutans caused by caries.
【作者單位】： 四川省醫(yī)學(xué)科學(xué)院·四川省人民醫(yī)院口腔科;大連醫(yī)科大學(xué)口腔醫(yī)院口腔內(nèi)科;口腔疾病研究國家重點(diǎn)實(shí)驗(yàn)室華西口腔醫(yī)院老年口腔科(四川大學(xué));
【基金】：四川省科技支撐計(jì)劃基金資助項(xiàng)目(2008FZ0173)
【分類號(hào)】：R781.5

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