本文選題：超順磁性支架材料 + 頜骨缺損　； 參考：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年03期

【摘要】：目的超順磁性材料在骨組織工程中的研究較少。文中旨在探討超順磁性支架材料聚乳酸-羥基乙酸(PLGA)/含鐵的羥基磷灰石顆粒(Fe-HA)修復(fù)兔下頜骨缺損的成骨效能并評(píng)估材料的生物相容性。方法隨機(jī)選取新西蘭大白兔36只,采用隨機(jī)數(shù)字表法分為6組:分別為術(shù)后非靜磁場(chǎng)飼養(yǎng)條件下的非靜磁場(chǎng)對(duì)照組(不植入材料),非靜磁場(chǎng)PLGA組(植入PLGA膜材料)、非靜磁場(chǎng)PLGA/Fe-HA組(植入PLGA/Fe-HA材料),靜磁場(chǎng)飼養(yǎng)條件下的靜磁場(chǎng)對(duì)照組(不植入材料)、靜磁場(chǎng)PLGA組(植入PLGA膜材料)、靜磁場(chǎng)PLGA/Fe-HA組(植入PLGA/Fe-HA材料),每組6只。制備雙側(cè)下頜骨缺損模型,分別于術(shù)后4、12周處死實(shí)驗(yàn)動(dòng)物,標(biāo)記并制備頜骨標(biāo)本,進(jìn)行大體觀察、Micro CT掃描、HE染色,并對(duì)缺損區(qū)骨密度值結(jié)果進(jìn)行分析。12周術(shù)后實(shí)驗(yàn)兔進(jìn)行血液學(xué)檢測(cè)、肝腎組織進(jìn)行病理檢驗(yàn)。結(jié)果非靜磁場(chǎng)PLGA/Fe-HA組、靜磁場(chǎng)PLGA/Fe-HA組4周時(shí)開始出現(xiàn)缺損區(qū)邊緣粗糙,缺損直徑減小,新生骨形成表現(xiàn);12周時(shí)缺損區(qū)形成光滑、連續(xù)的骨修復(fù)。非靜磁場(chǎng)PLGA組、靜磁場(chǎng)PLGA組12周時(shí)表現(xiàn)為粗糙、不整齊的骨痂修復(fù),缺損邊緣與正常骨組織邊界可見,非靜磁場(chǎng)對(duì)照組、靜磁場(chǎng)對(duì)照組缺損中心區(qū)域仍有部分骨缺損未修復(fù)。12周時(shí)與靜磁場(chǎng)PLGA/Fe-HA組骨密度值[(572.50±19.09)mg HA/cm3]比較,靜磁場(chǎng)PLGA組[(467.00±6.24)mg HA/cm3]、靜磁場(chǎng)對(duì)照組[(480.67±2.08)mg HA/cm3]、非靜磁場(chǎng)PLGA/Fe-HA組[(461.00±19.79)mg HA/cm3]、非靜磁場(chǎng)PLGA組[(446.00±11.31)mg HA/cm3]及非靜磁場(chǎng)對(duì)照組[(422.00±28.28)mg HA/cm3]均明顯降低(P0.05)。靜磁場(chǎng)PLGA/Fe-HA組4周時(shí)骨密度值[(572.50±19.09)mg HA/cm3]較12周的[(276.00±28.28)mg HA/cm3]明顯增加(P0.05),其他各組4周與12周組內(nèi)比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 PLGA/Fe-HA超順磁性支架材料可促進(jìn)骨缺損修復(fù),在磁場(chǎng)作用下材料的成骨效能被優(yōu)化,生物相容性尚可。
[Abstract]:Objective Superparamagnetic materials are rarely studied in bone tissue engineering. The purpose of this study was to investigate the osteogenic effect of superparamagnetic scaffold poly (lactic acid-glycolic acid) PLGAA / Fe-HA containing hydroxyapatite particles in repairing rabbit mandibular defects and to evaluate the biocompatibility of the materials. Methods Thirty-six New Zealand white rabbits were randomly selected. Six groups were randomly divided into six groups: the control group (non-static magnetic field control group), the non-static magnetic field (PLGA) group (implanted PLGA membrane material), the non-static magnetic field PLGA/Fe-HA group (PLGA/Fe-HA material implantation, static magnetic field group), the control group (non-static magnetic field control group), the non-static magnetic field PLGA group (implanted PLGA membrane material), the non-static magnetic field PLGA/Fe-HA group (implanted PLGA/Fe-HA material). Under the condition of magnetic field feeding, the static magnetic field control group (no implanted material), the static magnetic field PLGA group (implanted PLGA membrane material), the magnetostatic field PLGA/Fe-HA group (implanted PLGA/Fe-HA material, 6 rats in each group). The model of bilateral mandibular defect was made. Experimental animals were killed at 4 weeks and 12 weeks after operation. The specimens of jaws were labeled and prepared, and the micro CT scanning and HE staining were observed. The bone mineral density (BMD) of the defect area was analyzed. 12 weeks after operation, the experimental rabbits were examined by hematology, and the liver and kidney tissues were examined by pathology. Results in the non-static magnetic field PLGA/Fe-HA group, the edge of the defect began to appear at 4 weeks in the PLGA/Fe-HA group, the diameter of the defect decreased, and the defect area formed smooth and continuous bone repair at 12 weeks after the appearance of new bone formation. In the non-static magnetic field PLGA group, the static magnetic field PLGA group showed rough, irregular callus repair at 12 weeks, the defect edge was visible with the normal bone tissue boundary, and the non-static magnetic field control group. Bone mineral density (BMD) in the central defect area of the magnetostatic magnetic field control group [572.50 鹵19.09)mg HA/cm3] was compared with that in the magnetostatic magnetic field PLGA/Fe-HA group after 12 weeks of unrepaired bone defect. In the PLGA group, 480.67 鹵2.08)mg HA/cm3 group, 461.00 鹵19.79)mg HA/cm3 group, 446.00 鹵11.31)mg HA/cm3 group and 422.00 鹵28.28)mg HA/cm3 group, P0.05 was significantly decreased in the PLGA group, the control group [480.67 鹵2.08)mg HA/cm3], the non-static magnetic field PLGA/Fe-HA group [461.00 鹵19.79)mg HA/cm3], the non-static magnetic PLGA group [446.00 鹵11.31)mg HA/cm3] and the non-static magnetic field control group [422.00 鹵28.28)mg HA/cm3]. The bone mineral density (BMD) of the PLGA/Fe-HA group was significantly higher than that of the 12th week group [276.00 鹵28.28)mg HA/cm3] at the 4th week, and there was significant difference between the other groups in the 4th week and the 12th week group (P 0.05). Conclusion PLGA/Fe-HA superparamagnetic scaffold can promote the repair of bone defect, the osteogenic efficiency of the material is optimized under the action of magnetic field, and the biocompatibility is good.
【作者單位】： 南京大學(xué)醫(yī)學(xué)院附屬口腔醫(yī)院牙周科;南京大學(xué)醫(yī)學(xué)院附屬口腔醫(yī)院牙體牙髓科;南京大學(xué)醫(yī)學(xué)院附屬口腔醫(yī)院正畸科;
【基金】：國(guó)家自然科學(xué)基金(81570952,81300852) 江蘇省基金面上項(xiàng)目(BK20161114) 南京市醫(yī)學(xué)科技發(fā)展資金資助項(xiàng)目(YKK14109)
【分類號(hào)】：R782.4

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