發(fā)布時(shí)間：2018-05-02 06:18

  本文選題：口腔印模 + 復(fù)合季銨鹽��； 參考：《中國消毒學(xué)雜志》2017年07期

【摘要】：目的觀察以一種主要成分為芐索氯銨和異丙醇的口腔印模專用消毒劑對口腔印模表面乙肝病毒活性及免疫原性的影響。方法使用消毒液對HBV污染的口腔印模進(jìn)行噴霧消毒,檢測HBs Ag含量和HBV-DNA含量,評價(jià)消毒效果;檢測消毒前后HBV在體外誘導(dǎo)淋巴細(xì)胞分泌IFN-γ和IL-2含量,評價(jià)對HBV免疫能力的影響。結(jié)果噴霧消毒3 min和15 min,對HBs Ag和HBV-DNA的滅活效果明顯高于消毒1 min組,滅活率分別為(98.8±1.2)%、(98.9±1.1)%和(99.3±1.1)%、(99.4±1.3)%。消毒3 min和15 min HBV誘導(dǎo)淋巴細(xì)胞IFN-γ相對表達(dá)量明顯高于空白對照和未消毒組;與空白細(xì)胞組比較,未消毒組HBV誘導(dǎo)淋巴細(xì)胞IL-2相對表達(dá)量明顯增加,而各消毒組的IL-2相對表達(dá)量未發(fā)生顯著變化。結(jié)論該消毒劑噴涂口腔印模時(shí),對HBV的最佳消毒時(shí)間為3 min。此時(shí)HBV仍然保持較強(qiáng)的免疫原性,能夠誘導(dǎo)淋巴細(xì)胞IFN-γ表達(dá)水平上調(diào),對IL-2表達(dá)水平無影響。
[Abstract]:Objective to observe the effect of a special disinfectant with benzoxonium chloride and isopropanol on hepatitis B virus activity and immunogenicity on the surface of oral impression. Methods the disinfectant solution was used to disinfect the oral impression contaminated by HBV, to detect the content of HBs Ag and HBV-DNA, to evaluate the disinfection effect, to detect the content of IFN- 緯 and IL-2 in lymphocytes induced by HBV before and after disinfection, and to evaluate the effect on the immune ability of HBV. Results the inactivation of HBs Ag and HBV-DNA by spray disinfection for 3 min and 15 min was significantly higher than that in 1 min group, the inactivation rates were 98.8 鹵1.2% and 99.3 鹵1.1%, respectively. The relative expression of IFN- 緯 in lymphocytes induced by sterilizing 3 min and 15 min HBV was significantly higher than that of control group and unsterilized group, and the relative expression of IL-2 in lymphocytes induced by HBV in unsterilized group was significantly higher than that in control group and unsterilized group. However, there was no significant change in the relative expression of IL-2 in each disinfection group. Conclusion the best disinfection time of HBV is 3 min when the disinfectant is sprayed on oral impression. At the same time, HBV remained strong immunogenicity and could induce the up-regulation of IFN- 緯 expression in lymphocytes, but had no effect on the expression of IL-2.
【作者單位】： 湖州師范學(xué)院附屬口腔醫(yī)院;湖州師范學(xué)院附屬第一醫(yī)院;
【基金】：浙江省衛(wèi)計(jì)委臨床重點(diǎn)學(xué)科項(xiàng)目(W2014HB134)
【分類號】：R782.052

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本文編號：1832701