本文選題：脂肪干細胞 + 細胞膜片��； 參考：《第三軍醫(yī)大學學報》2017年23期

【摘要】：目的初步研究犬脂肪干細胞(adipose-derived stem cell,ADSCs)膜片的構(gòu)建及其生物學特性對膜片復合富血小板纖維蛋白(platelet-rich fibrin,PRF)后成骨能力的影響。方法取犬腹股溝處的脂肪進行消化,分離培養(yǎng)成脂肪干細胞進行相關檢測。將P3代細胞加入含抗壞血酸的成膜培養(yǎng)液培養(yǎng)2周以上,構(gòu)建脂肪干細胞膜片,采用HE染色及掃描電鏡對膜片進行形態(tài)學檢測。提取靜脈血PRF,分為細胞膜片復合PRF的實驗組和膜片對照組,2組分別成骨誘導后進行ALP染色和茜素紅染色,并于誘導后第3、5天,采用RT-PCR檢測成骨相關基因ALP、Runx2的表達。結(jié)果體外分離培養(yǎng)脂肪干細胞,成功構(gòu)建干細胞膜片。光鏡下可見細胞極性排列緊密,細胞外基質(zhì)分泌。掃描電鏡可見膜片表面光滑,細胞緊密排列。HE染色可見藍染細胞緊密相連,細胞外大量基質(zhì)。成骨誘導后,ADSCs膜片復合PRF的實驗組ALP表達及茜素紅染色鈣結(jié)節(jié)形成量明顯優(yōu)于ADSCs膜片對照組,實驗組ALP、Runx2基因m RNA表達高于對照組。結(jié)論成功構(gòu)建犬脂肪干細胞膜片,膜片復合PRF后具有更強的成骨能力。
[Abstract]:Objective to study the construction of adipose-derived stem cell (ADSCs) membrane and its biological characteristics on the osteogenic ability of dog adipose-derived stem cell (ADSCs) composite with platelet-rich fibrin (PRF).Methods the fat of dog groin was digested and the adipose stem cells were isolated and cultured.P3 generation cells were cultured in the medium containing ascorbic acid for more than 2 weeks to construct the adipose stem cell membrane. The morphology of the membrane was detected by HE staining and scanning electron microscope.PRFs were extracted from venous blood and divided into two groups: the experimental group with membrane membrane combined with PRF and the control group with ALP staining and alizarin red staining after osteogenesis induction. The expression of osteoblast-associated gene ALPnRunx2 was detected by RT-PCR 5 days after induction.Results Adipose stem cells were isolated and cultured in vitro.Under the light microscope, the cell polarity was closely arranged and extracellular matrix secreted.Scanning electron microscopy showed that the surface of the membrane was smooth and the cells were closely arranged. He staining showed that the blue stained cells were closely connected and a large amount of extracellular matrix was found.The expression of ALP and alizarin red stained calcium nodules in the experimental group was significantly higher than that in the ADSCs membrane control group after osteogenic induction, and the expression of ALP Runx2 gene m RNA in the experimental group was higher than that in the control group.Conclusion the canine adipose stem cell membrane was successfully constructed, and the membrane combined with PRF has stronger osteogenic ability.
【作者單位】： 重慶醫(yī)科大學附屬口腔醫(yī)院正畸科口腔疾病與生物醫(yī)學重慶市重點實驗室重慶市高校市級口腔生物醫(yī)學工程重點實驗室;重慶醫(yī)科大學附屬口腔醫(yī)院兒童口腔科口腔疾病與生物醫(yī)學重慶市重點實驗室重慶市高校市級口腔生物醫(yī)學工程重點實驗室;
【基金】：國家自然科學基金面上項目(81470772) 重慶市衛(wèi)計委醫(yī)學科研項目(20141013,2015HBRC009) 重慶市自然科學基金(CSTC2015jcyjA10028,CSTC2016jcyjA0238)~~
【分類號】：R781

【相似文獻】

相關期刊論文 前10條

1 陳芳;葉眉;姜大川;孫曉瑜;林瀟;沈繼龍;徐燕;;脂肪干細胞礦化中富血小板血漿的誘導作用及其在支架上附著增殖的研究[J];牙體牙髓牙周病學雜志;2009年01期

2 葉眉;徐燕;;脂肪干細胞在牙周組織工程學中的應用前景[J];口腔材料器械雜志;2009年01期

3 張健;楊晶;楊光艷;王慶福;沈軍;張?zhí)m成;;脂肪干細胞與人工骨混合后的成骨作用研究[J];組織工程與重建外科雜志;2012年03期

4 唐宇欣;金晗;史冊;朱陽;王丹丹;王賀;林崇韜;孫宏晨;;脂肪干細胞及其向成骨細胞分化的調(diào)控機制[J];國際口腔醫(yī)學雜志;2014年04期

5 陳慧;陳永進;張e，

本文編號：1758021