本文選題：白色念球菌　切入點(diǎn)：變形鏈球菌　出處：《大連醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：檢測(cè)根管再治療患者根管內(nèi)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌的檢出率情況，并分析再治療患者根管內(nèi)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌與臨床癥狀之間的關(guān)系。 方法：臨床選取131名根管根管再治療患者89顆患牙（四川大學(xué)華西口腔醫(yī)院），通過記錄患牙的臨床相關(guān)體征、癥狀等信息，去除根管內(nèi)原有充填物，，插入無(wú)菌紙尖60秒，與H銼刮取物一同放入EP管中，提取基因組DNA，通過PCR反應(yīng)檢測(cè)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌的16s RNA,檢測(cè)根管再治療患者根管內(nèi)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌的檢出率情況，并分析再治療患者根管內(nèi)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌與臨床癥狀之間的關(guān)系。結(jié)果采用SPSS13.0軟件進(jìn)行方差分析進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果：檢測(cè)根管再治療患者根管內(nèi)白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌的檢出率分別為：31.46%、29.21%、19.1%。在臨床癥狀中叩痛的病例組中的變形鏈球菌檢出率高于無(wú)叩痛的病例組（P＜0.05）。表明變形鏈球菌的檢出與臨床癥狀中的叩痛密切相關(guān)，與根尖周破壞呈正相關(guān)。 結(jié)論：1、根管再治療患者根管可以檢測(cè)出白色念球菌、變形鏈球菌、古細(xì)菌三種細(xì)菌。 2、根管再治療患者根管內(nèi)變形鏈球菌的檢出與臨床癥狀中的叩痛密切相關(guān)，與根尖周破壞呈正相關(guān)。
[Abstract]:Objective: to detect the detectable rate of Candida albicans, Streptococcus mutans and Archaea in root canal retreated patients, and to analyze the detection rate of Candida albicans and Streptococcus mutans in root canals of retreated patients.The relationship between three kinds of bacteria and clinical symptoms.Methods: 89 teeth were selected from 131 patients with root canal retreatment (Huaxi Stomatological Hospital, Sichuan University). By recording the clinical signs, symptoms and other information of the affected teeth, the original filling in the root canal was removed and the sterile paper tip was inserted into the root canal for 60 seconds.The genomic DNA was extracted from EP tube with H-file scraping. The 16s RNAs of Candida albicans, Streptococcus mutans and Archaea were detected by PCR reaction, and the Candida albicans and Streptococcus mutans in root canal retreated patients were detected.The detection rate of the three kinds of bacteria was analyzed and the relationship between the clinical symptoms and the clinical symptoms of Candida albicans, Streptococcus mutans and Archaea in the root canals of retreated patients was analyzed.Results the variance analysis was carried out by SPSS13.0 software.Results: the detectable rate of Candida albicans, Streptococcus mutans and Archaea in root canal retreated patients were respectively: 1. 31.46 and 29.21. 21.The detection rate of Streptococcus mutans in patients with percussive pain was significantly higher than that in patients without percussive pain (P < 0.05).The results showed that the detection of Streptococcus mutans was closely related to the percussive pain in clinical symptoms and positively related to periapical destruction.Conclusion in the patients with root canal retherapy, Candida albicans, Streptococcus mutans and archaea can be detected in the root canal.2. The detection of Streptococcus mutans in root canal was closely related to the percussion pain in clinical symptoms and positively related to periapical destruction.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R781.05


本文編號(hào)：1689001