本文選題：牙周膜　切入點(diǎn)：成纖維細(xì)胞　出處：《重慶醫(yī)學(xué)》2017年23期

【摘要】：目的研究白細(xì)胞介素17(IL-17)水平對(duì)人牙周膜成纖維細(xì)胞(HPDLF)中細(xì)胞核因子κB受體活化因子配體(RANKL)、骨保護(hù)素(OPG)表達(dá)的影響,探討IL-17與正畸牙根吸收的相關(guān)性。方法采用組織塊培養(yǎng)法,在體外建立人牙周膜成纖維細(xì)胞系。以不同水平(0、5、10、20、40ng/mL)的IL-17作用HPDLF 24h。采用RT-PCR和ELISA法,分別檢測(cè)RANKL和OPG的mRNA、蛋白的表達(dá)。結(jié)果 (1)在0ng/mL組中,HPDLF表達(dá)RANKL、OPG。(2)0~20ng/mL各組中,HPDLF中RANKL的mRNA、蛋白表達(dá)量與IL-17水平呈正相關(guān)性。(3)5~20ng/mL各組中,OPG的mRNA、蛋白表達(dá)量與IL-17水平呈負(fù)相關(guān)性。(4)0~20ng/mL組中,RANKL/OPG的mRNA、蛋白的比值與IL-17水平呈正相關(guān)性。結(jié)論在無(wú)IL-17刺激時(shí),HPDLF可表達(dá)RANKL、OPG。IL-17能夠促進(jìn)HPDLF表達(dá)RANKL,同時(shí)抑制HPDLF表達(dá)OPG,上調(diào)RANKL/OPG的比值。
[Abstract]:Objective to study the effect of interleukin 17 (IL 17) on the expression of nuclear factor 魏 B receptor activating factor ligand RANKL L (osteoprotegerin) in human periodontal ligament fibroblasts (HPD LF), and to explore the relationship between IL-17 and orthodontic root resorption. A human periodontal ligament fibroblast cell line was established in vitro. The HPDLF was treated with IL-17 at different levels (51010ng / mL) for 24 h. RT-PCR and ELISA methods were used. MRNAs and protein expressions of RANKL and OPG were detected respectively in 0ng/mL group. Results 1) in 0ng/mL group, the expression of RANKL in IL-17 was positively correlated with IL-17 level, and there was a negative correlation between protein expression and IL-17 level. The ratio of mRNA and protein of RANKL / OPG in 20 ng / mL group was positively correlated with the level of IL-17. Conclusion in the absence of IL-17 stimulation, the expression of RANKL / OPG.IL-17 can promote the expression of RANKL, inhibit the expression of HPDLF and up-regulate the ratio of RANKL/OPG.
【作者單位】： 廣西醫(yī)科大學(xué)附屬口腔醫(yī)院正畸科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81360170)
【分類(lèi)號(hào)】：R783.5

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本文編號(hào)：1668119