本文選題：-四氯二苯二英　切入點：胎鼠　出處：《重慶醫(yī)科大學學報》2017年12期 　論文類型：期刊論文

【摘要】：目的:總結歸納2,3,7,8-四氯二苯二英(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD)誘導胎鼠腭裂的實驗研究現(xiàn)狀,為深入探討TCDD致胎鼠腭裂的發(fā)生機制提供新的思路。方法:通過中國知網(wǎng)、NCBI等國內外文獻庫檢索"TCDD""腭裂"等關鍵詞,搜集已有文獻資料。結果:目前的資料顯示,可以通過約20μg/kg劑量的TCDD給予孕第9~14天的小鼠單次灌胃建立穩(wěn)定的胎鼠腭裂模型。在此基礎上發(fā)現(xiàn),TCDD誘導不涉及腭突體積變化,主要通過抑制腭突接觸融合而發(fā)揮致腭裂效應。對于發(fā)育中的腭組織細胞,TCDD能改變一系列調節(jié)細胞增殖、分化、凋亡的生長因子及其受體的表達,主要包括表皮細胞生長因子(epidermal growth factor,EGF)、轉化生長因子(transforming growth factor,TGF)、芳香烴受體(aryl hydrocarbon receptor,AhR)。這些分子的表達變化尚存在爭議。腭融合過程中發(fā)揮關鍵作用的腭中縫上皮細胞(medial edge epithelium,MEE),其細胞命運轉歸尚未闡明。結論:TCDD誘導胎鼠腭裂的體內實驗基礎相對完善。腭發(fā)育調控機制紛繁復雜,注重器官發(fā)育的時空動態(tài)性對于進一步研究至關重要。腭器官離體培養(yǎng)將是重要的體外實驗環(huán)節(jié)。實驗技術和條件的變革對于本領域的研究進展顯得愈加迫切。
[Abstract]:Objective: to summarize the present situation of the experimental study on the cleft palate induced by TCDD in the fetal rats. In order to provide a new idea for exploring the mechanism of cleft palate induced by TCDD, methods: the key words of "TCDD" and "cleft palate" were searched by the domestic and foreign literature database of TCDD. Results: the present data showed that the cleft palate might be caused by cleft palate. A stable cleft palate model of fetal mice could be established by a single dose of 20 渭 g / kg TCDD on the 14th day of gestation, and it was found that the volume of palatine process was not involved in the induction of TCDD. TCDD can change the expression of a series of growth factors and its receptors that regulate cell proliferation, differentiation and apoptosis. It mainly includes epidermal growth factor (EGFN), transforming growth factor (TGFN) and aryl hydrocarbon receptor (AhRN). The expression of these molecules is still in dispute. The medial edge epithelium MEE, which plays a key role in palatal fusion, plays a key role in the process of palatal fusion. Conclusion the in vivo experimental basis of fetal cleft palate induced by TCDD is relatively perfect, and the mechanism of palatal development regulation is complicated. The emphasis on spatiotemporal dynamics of organ development is very important for further research. In vitro culture of palatal organs will be an important experimental link in vitro. The changes of experimental techniques and conditions are becoming more and more urgent for the research progress in this field.
【作者單位】： 重慶醫(yī)科大學附屬兒童醫(yī)院燒傷整形科兒童發(fā)育疾病研究教育部重點實驗室重慶市兒科學重點實驗室重慶市兒童發(fā)育重大疾病診治與預防國際科技合作基地;
【基金】：國家自然科學基金青年基金資助項目(編號:81202167) 重慶市渝中區(qū)科技計劃資助項目(編號:20130121、20150112) 國家臨床重點�？平ㄔO資助項目(編號:國衛(wèi)辦醫(yī)函[2013]544)
【分類號】：R782.22

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本文編號：1621759