本文選題：血管再生　切入點：交感神經　出處：《福建醫(yī)科大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的:骨修復改建活動復雜而有序,與血管生成密不可分,它們相互影響和緊密聯系。以往認為骨代謝的調節(jié)涉及骨局部的自分泌、旁分泌以及內分泌等因素,然而越來越多的研究指出交感神經系統(tǒng)在骨形成和骨吸收過程中也發(fā)揮關鍵的作用。本研究通過檢測失交感神經支配下拔牙窩骨愈合過程中VEGF及CD31的表達特點,進一步探討血管再生在交感神經調節(jié)骨愈合過程中的可能作用。材料和方法:利用改良頸上神經切除術方法切除SD大鼠一側頸上神經節(jié),建立失交感神經支配的動物模型。拔除SD大鼠失交感神經側(SCGX側)與交感神經支配側(CG側)上頜第一磨牙,建立拔牙窩骨愈合動物模型。分別于拔牙術后1、3、7、14和21天5個時間點處死動物。通過免疫組化技術檢測CD31的表達,測定血管內皮細胞數目。并檢測拔牙窩底1/3、中1/3、頂1/3在拔牙后不同時間點VEGF的表達水平,測定其平均光密度值,進一步探討交感神經信號調節(jié)新骨形成和骨改建過程VEGF時空表達特點對血管再生的可能作用。結果:1、SD大鼠頸上神經節(jié)切除側表現出明顯的上瞼下垂的交感神經功能抑制的癥狀,免疫組化染色證實切除組織為頸上神經節(jié)組織。通過拔除SCGX側及CG側上頜第一磨牙,成功建立拔牙窩骨愈合模型。2、CD31免疫組化檢測結果表明:拔牙后第1天,SCGX組拔牙窩內CD31陽性染色的血管內皮細胞數量較CG組略多,但差異無統(tǒng)計學意義(P0.05);SCGX組在術后第3天、7天、14天時陽性表達的血管內皮細胞數量較CG組增加顯著,兩組差異有統(tǒng)計學意義(P0.05),在第21天時兩組血管內皮細胞數量均下降,且兩組數目接近,差異無統(tǒng)計學意義(P0.05)。3、VEGF表達的平均光密度值檢測結果表明:1)拔牙窩底1/3VEGF的表達:術后第1天,SCGx組的VEGF表達較對照組略高,但差異無統(tǒng)計學意義(P0.05);隨后VEGF表達增強,且第3天、7天、14天,兩組差異具有統(tǒng)計學意義(P0.05),而在第21天,兩組差異無統(tǒng)計學意義(P0.05)。2)拔牙窩中1/3VEGF的表達,SCGX組與CG組在術后第1天,表達較弱,兩組之間差異無統(tǒng)計學意義(P0.05),在拔牙后第3天、7天、14天、21天SCGx組的VEGF表達高于CG組,差異具有統(tǒng)計學意義(P0.05)。3)拔牙窩頂1/3VEGF的表達,SCGX組與CG組在術后第1天,第3天,兩組之間差異無統(tǒng)計學意義(P0.05),拔牙后第7天、14天、21天SCGx組的VEGF表達高于CG組,差異具有統(tǒng)計學意義(P0.05)。4)在拔牙窩VEGF整體表達來看,SCGX組與CG組在術后第1天,兩組之間差異無統(tǒng)計學意義(P0.05),在拔牙后第3天、7天、14天、21天兩組差異具有統(tǒng)計學意義(P0.05),整個拔牙窩的VEGF表達情況與拔牙窩中1/3相同。5)CG組的拔牙窩底部1/3與頂部1/3VEGF平均光密度比較,術后第1天、14天兩組間差異沒有統(tǒng)計學意義(P0.05),在術后第3天、7天、21天具有統(tǒng)計學意義(P0.05)。6)SCGX組的SD大鼠拔牙窩底部1/3與頂部1/3VEGF平均光密度比較,在術后第1天、3天、7天、21天,兩位點間差異有統(tǒng)計學意義(P0.05),而14天,兩位點差異無統(tǒng)計意義(P0.05)。結論:失交感神經支配調節(jié)拔牙窩愈合新骨形成的過程,可能是通過促進拔牙窩VEGF的表達,促進拔牙窩新骨形成區(qū)毛細血管新生,增加血管數量來改善拔牙窩的供血以改善微循環(huán),從而促進新骨形成。
[Abstract]:Objective: bone repair and reconstruction of complex and orderly, and angiogenesis are closely related, they affect each other and close contact. In the past that the regulation of bone metabolism involves autocrine bone, paracrine and endocrine factors, but more and more research is also pointed out that play a key role in the absorption process of the sympathetic nervous system in bone formation and bone. Expression of VEGF and CD31 in this study through the detection of sympathetic denervation by extraction socket healing process, to further explore the possible role in regulating bone vascular regeneration of sympathetic nerve in the healing process. Materials and methods: the cervical nerve resection by resection of SD rats to establish animal model of superior cervical ganglion, loss of sympathetic the innervation of the sympathetic nerve loss. Removal of SD rat side (SCGX side) and the sympathetic innervation side (CG side) of maxillary first molar, the establishment of the animal model of bone healing extraction sockets . respectively after extraction of 1,3,7,14 and 21 days 5 time points were animal. Using immunohistochemistry to detect the expression of CD31, vascular endothelial cell number and detection. The sockets at the bottom of the 1/3, 1/3, top 1/3 in the extraction at different time points after the expression of VEGF, determine the average optical density value, further study of sympathetic signal regulation of new bone formation and remodeling of the spatial and temporal expression of VEGF characteristics of the possible role of vascular regeneration. Results: 1 SD rat superior cervical ganglion resection side showed obvious function of sympathetic nerve ptosis inhibitory symptoms, immunohistochemical staining for tissue resection of superior cervical ganglion by tissue. Remove the SCGX side and the CG side of the maxillary first molar, successfully established the extraction socket healing model.2, CD31 immunohistochemistry results showed that: first days after extraction, SCGX extraction fossa CD31 positive staining the number of endothelial cells was lower than that of CG 緇勭暐澶，

本文編號：1557054