發(fā)布時(shí)間：2018-02-08 15:08

  本文關(guān)鍵詞： 紅景天苷 成骨細(xì)胞 低氧誘導(dǎo)因子 血管內(nèi)皮生長(zhǎng)因子　出處：《口腔醫(yī)學(xué)研究》2017年09期 　論文類型：期刊論文

【摘要】：目的:研究紅景天苷(SAL)對(duì)人成骨樣MG-63細(xì)胞增殖、分化和HIF-1α依賴的VEGF表達(dá)的影響及相關(guān)分子機(jī)制。方法:采用MTS法、流式細(xì)胞術(shù)分析、RT-PCR、ELISA、Western blot等技術(shù)觀察SAL對(duì)MG-63細(xì)胞增殖、分化和HIF-1α依賴的VEGF表達(dá)的影響,進(jìn)一步探討其相關(guān)分子機(jī)制。結(jié)果:SAL通過調(diào)控MG-63細(xì)胞的周期分布及上調(diào)Runx2和Osterix表達(dá)促進(jìn)成骨細(xì)胞的增殖和分化。另外,SAL可明顯上調(diào)下游靶基因VEGF的表達(dá)。結(jié)論:SAL可顯著促進(jìn)成骨樣MG-63細(xì)胞的增殖、分化以及HIF-1α依賴的VEGF的表達(dá),進(jìn)而促進(jìn)骨和血管新生。
[Abstract]:Objective: to study the effect of salidroside on the proliferation, differentiation and HIF-1 偽 dependent VEGF expression of human osteoblast like MG-63 cells and its molecular mechanism. Methods: the proliferation of MG-63 cells was observed by MTS and flow cytometry. Differentiation and the effect of HIF-1 偽 -dependent VEGF expression, Results the cell cycle distribution of MG-63 cells was regulated and the expression of Runx2 and Osterix was upregulated to promote the proliferation and differentiation of osteoblasts. In addition, Sal could significantly up-regulate the expression of downstream target gene VEGF. Conclusion\% Sal can promote the proliferation and differentiation of osteoblasts by regulating the cell cycle distribution and upregulating the expression of Runx2 and Osterix. It can significantly promote the proliferation of osteoblast-like MG-63 cells. Differentiation and HIF-1 偽-dependent VEGF expression promote bone and angiogenesis.
【作者單位】： 南開大學(xué)醫(yī)學(xué)院;武警后勤學(xué)院病原生物學(xué)與免疫學(xué)教研室;天津市口腔醫(yī)院正畸科;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):81273520、81502256、81572852) 國(guó)家臨床重點(diǎn)�？平ㄔO(shè)項(xiàng)目(口腔正畸專業(yè))批準(zhǔn)文號(hào):國(guó)衛(wèi)辦醫(yī)函[2013]544號(hào) 天津市口腔醫(yī)院博碩士重點(diǎn)項(xiàng)目(編號(hào):2015BSZD13)
【分類號(hào)】：R78
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本文編號(hào)：1495730