發(fā)布時間：2018-01-07 13:32

  本文關鍵詞：7種微生物在不同類型牙周病患者齦下菌斑中檢出率的研究　出處：《濱州醫(yī)學院》2015年碩士論文　論文類型：學位論文

  更多相關文章： 牙周炎 致病菌 多重PCR 16SrRNA

【摘要】：目的：檢測伴放線放線桿菌(Actinobacillus actinomycetemcomi tans,Aa)、牙齦卟啉單胞菌(Porphyromonas gingivalis,Pg)、福賽斯坦納菌(Tannerella forsythia,Tf).直腸彎曲桿菌(Campylobacter rectus,Cr)、侵蝕艾肯菌(Eikenella corrodens，Ec)、產黑普氏菌(Prevotella nigrescens,Pn)、古細菌(Archaea)在牙周健康者、菌斑性牙齦病患者及中重度慢性牙周炎患者齦下菌斑中的檢出率,探討這7種微生物與牙周病發(fā)生、發(fā)展的關系。方法：選取煙臺市口腔醫(yī)院牙周科就診患者中牙周健康者(A組)40例、菌斑性牙齦病者(B組)40例和中重度慢性牙周炎患者(C組)40例,收集齦下菌斑樣本,提取細菌DNA,以16SrRNA為基礎的特異性引物進行多重PCR技術和普通PCR技術檢測,記錄該7種微生物的檢出率,采用卡方檢驗進行統(tǒng)計學分析。結果：A,B組之間,Pn,Cr,Pg 3種微生物的檢出率存在顯著性差異(P0.05)。B,C組之間Ec,Cr,Pg,Tf,Aa,Archaea6種微生物的檢出率存在顯著性差異(P0.05),A、C組之間Ec,Pn,Cr,Pg,Tf,Aa,Archaea7種微生物檢出率存在顯著性差異(P0.05)。結論：Ec,Pn,Cr,Pg,Tf,Archaea在中重度慢性牙周炎患者中檢出率明顯高于健康者,說明其與中重度牙周炎具有顯著相關性。Ec,Cr,Pg,Tf,Archaea在中重度慢性牙周炎中檢出率顯著高于菌斑性牙齦病患者,說明其與牙周炎的進展程度相關。Pn,Cr,Pg在菌斑性牙齦病患者中檢出率顯著高于健康者,提示它們對牙齦炎癥有一定的敏感性。
[Abstract]:Objective: detection of Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomi, tans, Aa), Porphyromonas gingivalis (Porphyromonas gingivalis Pg), Forsyth Tanner Rand (Tannerella forsythia, Tf). The rectum Campylobacter (Campylobacter rectus, Cr), erosion (Eikenella corrodens, Ec Aiken bacteria), Prevotella nigrescens (Prevotella nigrescens, Pn), archaea (Archaea) in periodontal health, the detection rate of plaque induced gingival disease patients and patients with moderate and severe chronic periodontitis in subgingival plaque, of the 7 occurrence of periodontal disease and microorganisms, development of the relations. Methods: Yantai City Hospital of stomatology hospital patients periodontal healthy subjects (A group) 40 cases, plaque induced gingival disease (B group) and 40 cases of patients with moderate and severe chronic periodontitis (C group) 40 cases, collected subgingival plaque samples, bacterial DNA extraction, multi PCR technology and universal primers based on 16SrRNA are 閫歅CR鎶，

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