本文關(guān)鍵詞：咬肌力對(duì)大鼠下頜骨及髁突生長(zhǎng)發(fā)育的影響　出處：《南昌大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 曲安奈德 髁突 下頜骨 咬肌 IGF-Ⅰ

【摘要】：目的:通過兩種不同的方法處理咬肌,建立大鼠咬肌力減弱及咬肌力增強(qiáng)的動(dòng)物模型,并觀察這兩種方法對(duì)大鼠髁突及下頜骨生長(zhǎng)的影響。方法:48只21日齡SD大鼠,隨機(jī)分為3組,每組16只,于室內(nèi)溫度22℃、相對(duì)濕度為50%~60%、氣流速度≤0.2、氨濃度≤14mg/m3、噪聲≤60dB條件下喂養(yǎng)一周后,進(jìn)行試驗(yàn)。A組咬肌力增強(qiáng)組:大鼠咀嚼巧克力浸泡的木塊,以增強(qiáng)大鼠咬肌力量;B組咬肌力減弱組:采用醋酸曲安奈德0.01ml/100g處理大鼠咬肌,以使大鼠咬肌力減弱;C組對(duì)照組,不作任何處理。在建模第3周,第6周后每組分別處死大鼠8只,分別行下頜骨形態(tài)學(xué)測(cè)量及髁突組織學(xué)檢測(cè),并進(jìn)行胰島素樣生長(zhǎng)因子-Ⅰ(Insulin like growth factor-Ⅰ,IGF-Ⅰ)表達(dá)含量的檢測(cè),比較其在不同組及同組不同時(shí)間點(diǎn)表達(dá)的變化。結(jié)果:本實(shí)驗(yàn)成功建立了大鼠咬肌力增強(qiáng)及咬肌力減弱的實(shí)驗(yàn)?zāi)Ｐ�。⑴下頜骨形態(tài)學(xué)測(cè)量顯示:①:下頜骨長(zhǎng)度測(cè)量結(jié)果顯示:B組(咬肌力減弱組)大鼠下頜骨長(zhǎng)度明顯小于A組(咬肌力增強(qiáng)組)大鼠下頜骨長(zhǎng)度;C組(對(duì)照組)大鼠下頜骨長(zhǎng)度明顯小于A組(咬肌力增強(qiáng)組)大鼠下頜骨長(zhǎng)度;C組(對(duì)照組)大鼠下頜骨長(zhǎng)度大于B組(咬肌力減弱組)大鼠下頜骨長(zhǎng)度,三者兩兩比較,差別有統(tǒng)計(jì)學(xué)意義(p0.05)。②:下頜升支高度測(cè)量結(jié)果顯示:A組大鼠下頜支高度大于B組與C組大鼠下頜支高度;C組大鼠下頜支高度要明顯大于B組大鼠下頜支高度,差別有統(tǒng)計(jì)學(xué)意義(p0.05)。③:下頜角角度測(cè)量結(jié)果顯示:A組大鼠下頜角度大于C組大鼠下頜角度,C組大鼠下頜角度大于B組大鼠下頜角度,差別有統(tǒng)計(jì)學(xué)意義(p0.05)。④:下頜體高度測(cè)量結(jié)果顯示:A組,B組,C組兩兩比較,差別無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)。⑵髁突組織學(xué)檢測(cè)結(jié)果顯示:①:咬肌力增強(qiáng)組大鼠髁突軟骨各細(xì)胞層分層明顯,細(xì)胞排列密集;②:咬肌力減弱組大鼠髁突軟骨各細(xì)胞層細(xì)胞排列稀疏;③:對(duì)照組髁突軟骨各細(xì)胞層排列稀疏,相對(duì)于咬肌力減弱組而言,細(xì)胞排列較密集。⑶髁突軟骨免疫組化結(jié)果顯示,IGF-Ⅰ主要表達(dá)于成軟骨細(xì)胞層和肥大細(xì)胞層。IGF-1蛋白半定量結(jié)果顯示:IGF-1因子在髁突軟骨細(xì)胞中的表達(dá)率,增殖層,成軟骨細(xì)胞層,肥大層陽(yáng)性細(xì)胞表達(dá)術(shù)后三周要強(qiáng)于術(shù)后六周;IGF-1因子在增殖層的表達(dá)表現(xiàn)為同一時(shí)刻A、B、C三組表達(dá)有差異,咬肌力增強(qiáng)組的表達(dá)強(qiáng)于對(duì)照組,對(duì)照組強(qiáng)于咬肌力減弱組。結(jié)論:1:在本實(shí)驗(yàn)環(huán)境條件下,咬肌力大時(shí),有利于髁突及下頜骨的生長(zhǎng),并使下頜角角度減小。咬肌力減弱時(shí),下頜角角度增大。2:在本實(shí)驗(yàn)環(huán)境條件下,髁突軟骨各細(xì)胞層細(xì)胞的分化與壓力的大小呈正相關(guān)。3:IGF-Ⅰ因子作為一種生長(zhǎng)因子,在生長(zhǎng)發(fā)育期間,應(yīng)力越大,IGF-Ⅰ因子陽(yáng)性表達(dá)率越高,IGF-Ⅰ因子可以作為檢測(cè)髁突生長(zhǎng)發(fā)育的指標(biāo)。
[Abstract]:Objective: through two different methods of processing the masseter muscle, muscle weakness and a bite bite strength of the animal model of rats, and observe the effects of the two methods on the growth of condyle and mandible in rats. Methods: 48 21 day old SD rats were randomly divided into 3 groups, 16 rats in each group, in the room temperature of 22 degrees Celsius, relative humidity is 50%~60%, flow rate is less than 0.2, the ammonia concentration is less than or equal to 14mg/m3, a week after the noise is less than or equal to 60dB under the condition of feeding, test group.A bite strength group: rats were chewing chocolate soaked wood, in order to enhance the strength of masseter muscle weakness; group B group: bite by acetic acid 0.01ml/100g triamcinolone treated rats masseter muscle, to make rat bite muscle weakness; group C control group, without any treatment. In third weeks, sixth weeks after modeling, rats in each group were sacrificed at 8, respectively, the Mandible Condyle morphology measurement and histological examination, and insulin-like growth factor-I (Insu Lin like growth factor- I, IGF- I) expression were detected, comparing the changes in different groups and different time points in group expression. Results: we have successfully established experimental model of rat bite bite strength and muscle weakness. The mandibular morphology measurement showed: 1: the measurement results showed that the length of mandible B group (bite muscle weakness group) rat mandibular length is less than the A group (bite strength group) rat mandibular length; C group (control group) the length of mandible in rats were significantly less than group A (bite strength group) rat mandibular length; C group (control group) of rat mandibular length is greater than the B group (bite muscle weakness group) rat mandibular length, 22 compared with three, the difference was statistically significant (P0.05). The mandibular ramus height measurement results showed that A group rats mandibular ramus height greater than B group and C group rat mandibular ramus height; C rats mandibular ramus height to Ming Rats in B group were significantly larger than the height of the mandibular ramus, the difference was statistically significant (P0.05). The mandibular angle measurement results showed that: A rats mandibular angle is greater than the C rats C rats mandibular angle, mandibular angle is greater than the group B rat mandibular angle, the difference was statistically significant (P0.05). 4. The results show: mandibular height measurement: A group, B group, C group 22, the difference was not statistically significant (P0.05). The condylar histological examination of the results showed that: bite strength in condylar cartilage of rats in all cell layers increased, cells arranged densely; the bite muscle weakness of condyle cartilage of rats at each cell layer cells are sparse; the control group: the condylar cartilage layers are sparse, with respect to bite muscle weakness group, cells arranged indense. The condylar cartilage immunohistochemistry results showed that IGF- I mainly expressed in chondrogenic cell layer and the layer of.IGF-1 protein half mast cells The quantitative results showed that the expression of factor IGF-1 in condylar cartilage cells proliferation rate, layer, chondroblast layer, hypertrophic layer positive cells after three weeks than 6 weeks after surgery; IGF-1 factor showed the same time A expression in proliferative layer B, C three groups with different expression, bite strength enhanced expression of strong group than in the control group, the control group is stronger than the bite muscle weakness group. Conclusion: 1: under the experimental conditions, bite strength, is conducive to the growth of condyle and mandible, and the mandibular angle decreases. Bite muscle weakness, mandibular angle increased.2: in this experimental environment under the size of.3:IGF- was positively related to differentiation factor and pressure of condylar cartilage layers of cells as a growth factor in the growth period, the greater the stress factor, IGF- positive expression rate is high, the IGF- factor can be used to measure the condylar growth index.

【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R783.5

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