孤獨癥大鼠前額葉皮質(zhì)神經(jīng)元凋亡相關(guān)蛋白的表達
發(fā)布時間：2019-03-25 17:57
【摘要】：目的觀察孤獨癥大鼠前額葉皮質(zhì)神經(jīng)元凋亡相關(guān)蛋白Caspase-3和Bcl-2表達的變化,探討細胞凋亡在孤獨癥發(fā)病中的作用。方法健康繁殖期Wistar雌鼠20只,體質(zhì)量250~260 g,隨機選取10只在E12.5腹腔注射丙戊酸鈉(VPA),其子代為孤獨癥模型組;其余10只在E12.5腹腔注射生理鹽水,其子代為對照組。通過比較負向性實驗、自梳理實驗驗證模型是否成功;Western blotting方法對比對照組與孤獨癥模型組大鼠P42前額葉皮質(zhì)凋亡相關(guān)蛋白Caspase-3和Bcl-2表達的變化。結(jié)果成功建立孤獨癥動物模型。與對照組比較,孤獨癥模型組大鼠旋轉(zhuǎn)調(diào)頭時間顯著延長(P0.05),理毛時間顯著增加(P0.05);P42前額葉皮質(zhì)凋亡相關(guān)蛋白Caspase-3表達顯著降低(P0.05),Bcl-2表達顯著升高(P0.05)。結(jié)論孤獨癥大鼠P42前額葉皮質(zhì)凋亡受到抑制,提示調(diào)節(jié)凋亡可能是一個潛在的孤獨癥治療策略。
[Abstract]:Objective To observe the changes of the expression of Caspase-3 and Bcl-2 in the prefrontal cortex of autistic rats, and to explore the role of apoptosis in the pathogenesis of autism. Methods Twenty-two female Wistar rats were randomly selected to be injected with sodium valproate (VPA) in the abdominal cavity of E12.5. The rest of the 10 rats were injected with normal saline in the abdominal cavity of E12.5, and their children were in the control group. The expression of Caspase-3 and Bcl-2 in the prefrontal cortex of P42 in the control group and the autistic model group was compared by Western blotting. Results The autistic animal model was successfully established. The expression of Caspase-3 in the prefrontal cortex of P42 was significantly lower (P0.05), and the expression of Bcl-2 was significantly increased (P0.05). Conclusion The apoptosis of P42 prefrontal cortex of autistic rats is inhibited, and it is suggested that the regulation of apoptosis may be a potential autistic treatment strategy.
【作者單位】： 貴陽醫(yī)學院人體解剖學教研室;貴陽市第二人民醫(yī)院神經(jīng)內(nèi)科;貴陽醫(yī)學院附屬醫(yī)院兒科;貴陽醫(yī)學院生物技術(shù)教研室;
【基金】：貴州市科技計劃項目[筑科合同(2011103)18號] 貴州省科技攻關(guān)計劃課題[黔科合SY(2010)3082號]
【分類號】：R749.94
[Abstract]:Objective To observe the changes of the expression of Caspase-3 and Bcl-2 in the prefrontal cortex of autistic rats, and to explore the role of apoptosis in the pathogenesis of autism. Methods Twenty-two female Wistar rats were randomly selected to be injected with sodium valproate (VPA) in the abdominal cavity of E12.5. The rest of the 10 rats were injected with normal saline in the abdominal cavity of E12.5, and their children were in the control group. The expression of Caspase-3 and Bcl-2 in the prefrontal cortex of P42 in the control group and the autistic model group was compared by Western blotting. Results The autistic animal model was successfully established. The expression of Caspase-3 in the prefrontal cortex of P42 was significantly lower (P0.05), and the expression of Bcl-2 was significantly increased (P0.05). Conclusion The apoptosis of P42 prefrontal cortex of autistic rats is inhibited, and it is suggested that the regulation of apoptosis may be a potential autistic treatment strategy.
【作者單位】： 貴陽醫(yī)學院人體解剖學教研室;貴陽市第二人民醫(yī)院神經(jīng)內(nèi)科;貴陽醫(yī)學院附屬醫(yī)院兒科;貴陽醫(yī)學院生物技術(shù)教研室;
【基金】：貴州市科技計劃項目[筑科合同(2011103)18號] 貴州省科技攻關(guān)計劃課題[黔科合SY(2010)3082號]
【分類號】：R749.94
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