【摘要】：目的研究慢性不可預知溫和應激(UCMS)對SAMP8小鼠抑郁情緒、學習記憶能力的影響,及UCMS對海馬中腦源性神經(jīng)營養(yǎng)因子(BDNF)及其受體(Trk B)、神經(jīng)元凋亡相關(guān)因子B淋巴細胞瘤-2基因(Bcl-2)、Bcl-2相關(guān)X蛋白(Bax)蛋白表達的影響。方法選取健康、7月齡、雄性SAMP8小鼠20只,對其進行隨機分組,分為應激組和非應激組,每組各10只。然后對兩組小鼠進行為期21天的慢性不可預知應激,應激完成后再進行水迷宮實驗及糖水偏好試驗。行為學實驗完成后,應激組和非應激組各隨機選取6只小鼠進行斷頭取海馬,采用蛋白質(zhì)印跡法(westernblotting)對BDNF及Trk B、Bcl-2、Bax的蛋白表達量進行測定。結(jié)果1經(jīng)過5天的訓練后,應激組小鼠尋找到平臺的潛伏期明顯增長(P0.05);在第6天撤除平臺后,應激組小鼠穿越平臺次數(shù)比非應激組明顯減少(P0.05)。2應激后應激組小鼠比非應激組糖水消耗量明顯下降(P0.05)。3與非應激組比較,應激組小鼠BDNF及Trk B蛋白表達明顯減少,且具有統(tǒng)計學意義(P0.05)。4與非應激組比較,應激組小鼠海馬Bcl-2蛋白表達明顯減少,Bax蛋白表達明顯增多,且具有統(tǒng)計學意義(P0.05)。結(jié)論應激使SAMP8小鼠產(chǎn)生抑郁情緒、學習記憶能力下降;應激導致的BDNF及其Trk B、Bcl-2蛋白表達量減少,Bax蛋白量表達增多。
[Abstract]:Objective to study the effects of chronic unpredictable mild stress (UCMS) on depression, learning and memory ability in SAMP8 mice, and the effect of UCMS on brain-derived neurotrophic factor (BDNF) and its receptor (Trk B), in hippocampus. Effect of apoptosis-related factor B lymphocyte tumor-2 gene (Bcl-2) and Bcl-2-associated X protein (Bax) protein expression. Methods Twenty healthy 7-month-old male SAMP8 mice were randomly divided into two groups: stress group (n = 10) and non-stress group (n = 10). Two groups of mice were subjected to chronic unpredictable stress for 21 days. After the stress was completed, the water maze test and sugar-water preference test were performed. After the behavioral experiment was completed, six mice in the stress group and the non-stress group were randomly selected and the hippocampus was decapitated. The protein expression of BDNF, Trk-B, Bcl-2 and Bax were measured by protein blot (westernblotting). Results (1) after 5 days of training, the latency of finding the platform in the stress group increased significantly (P0.05). After removing the platform on the 6th day, the number of crossing the platform in the stress group was significantly lower than that in the non-stress group (P0.05). 2 the consumption of sugar water in the stress group was significantly lower than that in the non-stress group (P0.05). 3Compared with the non-stress group, the frequency of crossing the platform in the stress group decreased significantly (P0.05). (4) compared with non-stress group, the expression of BDNF and Trk B protein in hippocampus of stress group decreased significantly, and the expression of Bax protein increased significantly, compared with non-stress group, the expression of Bcl-2 and Bax protein in hippocampus of stress group was significantly decreased, and the expression of Bax protein was significantly higher than that of non-stress group. And has statistical significance (P0.05). Conclusion stress induced depression and decreased the ability of learning and memory in SAMP8 mice, the expression of Bcl-2 protein and Bcl-2 protein decreased and the expression of Bax protein increased in BDNF and Trk-B induced by stress.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R749.4

【參考文獻】

相關(guān)期刊論文 前10條

1 沈忠飛;王志堅;潘巍巍;郭燕君;伏春艷;袁鳳鳳;;氟西汀調(diào)控CUMS抑郁大鼠海馬突觸重塑[J];中國病理生理雜志;2016年09期

2 王戈;任路;賈連群;;電針對圍絕經(jīng)期抑郁癥大鼠海馬Bcl-2和Bax的影響[J];中國中醫(yī)基礎(chǔ)醫(yī)學雜志;2016年06期

3 王含彥;唐珍;鄧志霖;劉文芹;楊夢茹;陳月琦;劉靜;曾安蓉;;氟西汀對慢性不可預知性輕度應激抑郁模型大鼠下丘腦—垂體—腎上腺軸的影響[J];蘭州大學學報(醫(yī)學版);2016年01期

4 王宇紅;柴上;譚小雯;楊蕙;孟盼;張秀麗;;左歸降糖解郁方對糖尿病并發(fā)抑郁癥大鼠海馬Bcl-2,Bax,Caspase 8蛋白表達的影響[J];中國實驗方劑學雜志;2015年03期

5 高文婷;楊策;王海燕;杜娟;蔣建新;;應激模型的生理學基礎(chǔ)及其比較研究進展[J];現(xiàn)代生物醫(yī)學進展;2014年21期

6 劉加強;童衛(wèi);;海馬神經(jīng)發(fā)生障礙與阿爾茲海默病發(fā)病機制[J];神經(jīng)解剖學雜志;2014年02期

7 馬學萍;安書成;;慢性應激性抑郁發(fā)生中海馬糖皮質(zhì)激素對BDNF的影響[J];陜西師范大學學報(自然科學版);2013年06期

8 王涵;李娜;文威;姜蓉;周岐新;;慢性應激致大鼠抑郁行為涉及海馬形態(tài)變化和BDNF表達降低[J];基礎(chǔ)醫(yī)學與臨床;2011年05期

9 鄭剛;駱文靜;陳耀明;劉明朝;馬金龍;陳景元;;慢性復合應激對大鼠學習記憶功能及腦ERK表達活化的影響[J];中國應用生理學雜志;2011年01期

10 苑振云;顧平;劉靜;王銘維;;快速老化動物模型SAMP_8小鼠及其相關(guān)研究進展[J];中國組織工程研究與臨床康復;2008年46期

相關(guān)碩士學位論文 前1條

1 陳美玲;急慢性給予艾司西酞普蘭對慢性不可預知溫和應激小鼠行為學的影響[D];昆明醫(yī)學院;2009年

，

本文編號：2432063