【摘要】：脂多糖(lipopolysaccharide,LPS)免疫激活模型是研究抑郁癥細(xì)胞因子假說的重要?jiǎng)游锬Ｐ?目前國(guó)際上常用外周單次LPS注射誘發(fā)抑郁樣行為,但該模型中抑郁樣行為持續(xù)僅有數(shù)小時(shí)。為建立誘發(fā)較長(zhǎng)時(shí)程抑郁樣行為的免疫激活動(dòng)物模型,本研究嘗試側(cè)腦室注射LPS激活大鼠中樞免疫炎性反應(yīng),考察單次以及重復(fù)中樞LPS注射誘發(fā)抑郁樣行為的效果,以及中樞炎性免疫誘發(fā)行為改變的時(shí)程。結(jié)果顯示:單次中樞LPS注射后24 h只能誘發(fā)曠場(chǎng)自發(fā)活動(dòng)和探索行為下降等部分抑郁樣行為,未能誘導(dǎo)糖水偏好下降和懸尾不動(dòng)時(shí)間增加;3次重復(fù)注射則在末次LPS注射后24 h表現(xiàn)出顯著的糖水偏好下降,自發(fā)活動(dòng)和探索行為減少,懸尾不動(dòng)時(shí)間增加等行為。且自發(fā)活動(dòng)、探索行為減少和懸尾不動(dòng)時(shí)間增加能夠延續(xù)至末次LPS注射后72 h。這些結(jié)果表明腦室重復(fù)LPS注射可誘發(fā)較長(zhǎng)時(shí)程的抑郁樣行為,這種新的中樞炎性免疫激活誘發(fā)的抑郁癥模型,為研究抑郁癥炎性免疫機(jī)制提供了更為有效的動(dòng)物模型,有助于深入探討行為和免疫功能間的復(fù)雜關(guān)系。
[Abstract]:Lipopolysaccharide (LPS) immunoreactive model is an important animal model to study the cytokine hypothesis of depression. At present, depression like behavior is induced by single injection of LPS in the world, but the depressive behavior in this model lasts only a few hours. In order to establish an immune-activated animal model of long-lasting depressive behavior, we tried to induce the central immune inflammatory response in rats by intraventricular injection of LPS, and to investigate the effect of single and repeated central LPS injection on depressive behavior in rats. And the time course of behavior change induced by central inflammatory immunity. The results showed that 24 h after a single injection of LPS, only some depressive behaviors such as spontaneous open field activity and decreased exploratory behavior were induced. The decrease of sugar water preference and the increase of tail suspension immobility time after 24 hours of LPS injection showed significant decrease of sugar water preference, decrease of spontaneous activity and exploration behavior, and increase of tail suspension immobility time. Spontaneous activity, decreased exploratory behavior and increased tail immobility could last until 72 hours after the last LPS injection. These results suggest that repeated injection of LPS can induce long-term depressive behavior. This new model of depression induced by central inflammatory immune activation provides a more effective animal model for studying the inflammatory immune mechanism of depression. It is helpful to explore the complex relationship between behavior and immune function.
【作者單位】： 中國(guó)科學(xué)院心理健康重點(diǎn)實(shí)驗(yàn)室 中國(guó)科學(xué)院心理研究所;中國(guó)科學(xué)院大學(xué);
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(30770718,31170987) 中國(guó)科學(xué)院知識(shí)創(chuàng)新項(xiàng)目(KSCX2-EW-J-8)資助
【分類號(hào)】：R749.4

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