本文選題：�；撬� + 慢性應(yīng)激��； 參考：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：慢性輕度不可預(yù)見(jiàn)應(yīng)激(Chronic mild unforeseeable stress,CUMS)可造成海馬神經(jīng)元的損傷,加速神經(jīng)元凋亡,誘發(fā)抑郁癥、阿爾茲海默癥等神經(jīng)退行性疾病。�；撬岬幕瘜W(xué)性質(zhì)穩(wěn)定,對(duì)神經(jīng)系統(tǒng)具有營(yíng)養(yǎng)和保護(hù)作用,可促進(jìn)大腦發(fā)育,增強(qiáng)記憶。本研究旨在探討�；撬釋�(duì)CUMS大鼠海馬神經(jīng)凋亡及再生的調(diào)控的作用,為臨床上應(yīng)用�；撬岱乐紊窠�(jīng)退行性疾病提供一定的理論依據(jù)。本試驗(yàn)采用SPF級(jí)Wistar大鼠構(gòu)建CUMS模型,將試驗(yàn)大鼠隨機(jī)分為六組:空白對(duì)照組(N);�；撬釋�(duì)照組(TⅠ、TⅡ);慢性應(yīng)激組(M);�；撬岣深A(yù)組(TⅠ+M、TⅡ+M)。每周末稱(chēng)量大鼠體重,并進(jìn)行糖水偏好試驗(yàn),驗(yàn)證造模效果。28天試驗(yàn)結(jié)束后,進(jìn)行曠場(chǎng)試驗(yàn)及Morris水迷宮試驗(yàn)檢測(cè)�；撬釋�(duì)CUMS大鼠行為學(xué)的影響。行為學(xué)試驗(yàn)結(jié)束后,采用心臟灌流固定法取大腦,采用免疫組織化學(xué)染色法檢測(cè)神經(jīng)再生標(biāo)記物ki67及凋亡相關(guān)因子表達(dá),TUNEL法檢測(cè)海馬神經(jīng)元凋亡情況;同時(shí),采用qRT-PCR及Western-blot法檢測(cè)內(nèi)源性細(xì)胞凋亡通路調(diào)控因子Caspase-3、Caspase-9、Bax和Bcl-2的mRNA及蛋白表達(dá)水平。結(jié)果顯示,隨著應(yīng)激模型的制備,慢性應(yīng)激組大鼠對(duì)糖水偏愛(ài)程度降低,同空白對(duì)照組與�；撬岣深A(yù)組相比,均差異極顯著(p0.01),慢性應(yīng)激組大鼠體重增長(zhǎng)緩慢;曠場(chǎng)試驗(yàn)中,慢性應(yīng)激組大鼠對(duì)新異環(huán)境的探索能力降低,在中央?yún)^(qū)域停留時(shí)間減少,周?chē)鷧^(qū)域停留時(shí)間顯著增加,與空白對(duì)照組和�；撬岣深A(yù)組相比,差異顯著(p0.05),同時(shí),慢性應(yīng)激組大鼠表現(xiàn)焦灼,排便次數(shù)較多,水平運(yùn)動(dòng)和垂直運(yùn)動(dòng)得分低于空白對(duì)照組與�；撬岣深A(yù)組,差異極顯著(p0.01);水迷宮試驗(yàn)中,慢性應(yīng)激組大鼠,逃避潛伏期及移動(dòng)距離增加,穿越平臺(tái)次數(shù)較少,同空白對(duì)照組與�；撬岣深A(yù)組相比,均差異顯著(p0.01或p0.05);免疫組化結(jié)果顯示,慢性應(yīng)激組DG區(qū)顆粒下層神經(jīng)元凋亡率顯著增加,Caspase-3、Caspase-9、Bax的表達(dá)顯著高于空白對(duì)照組及�；撬岣深A(yù)組(p0.01或p0.05);凋亡抑制因子Bcl-2的表達(dá)顯著低于空白對(duì)照組及�；撬岣深A(yù)組(p0.01或p0.05);慢性應(yīng)激組大鼠海馬神經(jīng)再生標(biāo)記物Ki67的表達(dá)極顯著低于空白對(duì)照組與牛磺酸干預(yù)組(p0.01)。qRT-PCR及Western-blot結(jié)果顯示,慢性應(yīng)激組大鼠凋亡相關(guān)因子Caspase-3、Caspase-9、Bax的mRNA及蛋白的表達(dá)量顯著高于空白對(duì)照組及牛磺酸干預(yù)組(p0.01或p0.05);慢性應(yīng)激組大鼠凋亡抑制因子Bcl-2的mRNA及蛋白表達(dá)量顯著低于空白對(duì)照組及�；撬岣深A(yù)組(p0.01或p0.05)。本試驗(yàn)結(jié)果表明,牛磺酸可通過(guò)調(diào)控內(nèi)源性凋亡通路抑制CUMS導(dǎo)致的大鼠海馬神經(jīng)元異常凋亡,增加神經(jīng)再生,進(jìn)而改善由CUMS導(dǎo)致的快感缺失、學(xué)習(xí)記憶能力下降、焦慮恐懼等行為學(xué)障礙。
[Abstract]:Chronic mild unforeseeable stress (CUMS) can cause neuronal damage, accelerate neuronal apoptosis, induce depression, Alzheimer's disease and other neurodegenerative diseases. Taurine has stable chemical properties and has nutritional and protective effects on nervous system. It can promote brain development and enhance memory. The purpose of this study was to investigate the effect of taurine on apoptosis and regeneration of hippocampal nerve in CUMS rats, and to provide a theoretical basis for the clinical application of taurine in the prevention and treatment of neurodegenerative diseases. The CUMS model was established by SPF Wistar rats. The rats were randomly divided into six groups: blank control group (N), taurine control group (T 鈪，

本文編號(hào)：2104662