本文選題：環(huán)磷酸腺苷反應元件結合蛋白 + 慢性應激�。� 參考：《中國神經精神疾病雜志》2014年01期

【摘要】：目的探討慢性應激狀態(tài)下,以及持續(xù)給予鹽酸氟西汀后,大鼠海馬及前額葉部位環(huán)磷酸腺苷反應元件結合蛋白(cAMP-response element binding protein,CREB)水平和磷酸化CREB(p-CREB)表達的變化。方法成年雄性Sprague-Dawley(SD)大鼠50只隨機分為三組:應激組26只,藥物組12只,對照組12只。應激組與藥物組大鼠均經過8周慢性應激刺激,藥物組在第1周開始給予鹽酸氟西汀(10mg/g,I.P.),直到8周末。8周后行糖水偏好實驗和曠場實驗,根據8周末糖水偏愛率將應激組大鼠分為應激敏感組和應激適應組。應激結束后將四組大鼠斷頭處死,采用蛋白免疫印記技術檢測大鼠海馬、額葉部位CREB和p-CREB水平。結果 8周末應激敏感組糖水偏愛率低于應激適應組、藥物組及對照組(均P0.05);應激敏感組的跨格次數、中心格時間和直立次數低于藥物組和對照組(均P0.05),與應激適應組無統(tǒng)計學差異(均P0.05)。應激敏感組海馬及前額葉中p-CREB水平明顯低于應激適應組、藥物組及對照組(均P0.05);四組大鼠海馬部位、前額葉CREB表達水平無明顯差異。結論海馬和額葉部位CREB磷酸化水平可能與應激導致的情緒障礙發(fā)生機制相關。
[Abstract]:Objective to investigate the level of cyclic adenosine monophosphate (camp) response element binding protein (cAMP-response element binding protein) and the expression of phosphorylated CREB-p-CREBin in hippocampus and prefrontal lobe of rats under chronic stress and after continuous administration of fluoxetine hydrochloride. Methods 50 adult male Sprague-Dawley SD rats were randomly divided into three groups: stress group (n = 26), drug group (n = 12) and control group (n = 12). The rats in the stress group and the drug group were stimulated by chronic stress for 8 weeks. The drug group was given fluoxetine hydrochloride 10 mg / g / g I.P.E at the first week, and the sugar water preference test and open field experiment were performed after the 8th week. Rats in stress group were divided into stress sensitive group and stress adaptation group according to sugar water preference rate at the end of 8 weeks. After the stress, the rats in the four groups were killed and the levels of CREB and p-CREB in hippocampus and frontal lobe were detected by protein immunoimprint technique. Results at the end of 8 weeks, the sugar water preference rate of stress sensitive group was lower than that of stress adaptation group, drug group and control group (all P 0.05). The time of center lattice and the times of standing upright were lower than those of drug group and control group (P 0.05), but there was no significant difference between the control group and the stress adaptation group (all P 0.05). The levels of p-CREB in hippocampus and prefrontal lobe in stress sensitive group were significantly lower than those in stress adaptation group, drug group and control group (all P 0.05). Conclusion the level of CREB phosphorylation in hippocampus and frontal lobe may be related to the mechanism of emotional disorder induced by stress.
【作者單位】： 上海交通大學醫(yī)學院附屬精神衛(wèi)生中心心境障礙科;航天中心醫(yī)院神經內科;復旦大學附屬華山醫(yī)院中西醫(yī)結合研究所;
【基金】：上海交通大學科學基金(編號:11XJ21006) 上海市自然科學基金(編號:09ZR1427200) 上海市衛(wèi)生局公共衛(wèi)生海外人才項目(編號:GWHW201208) 國家高技術研究發(fā)展計劃“863”計劃(編號:2006AA02Z430) 國家自然科學基金重大計劃項目(編號:91232719) 國家臨床重點�？�-上海市精神衛(wèi)生中心(編號:衛(wèi)生部醫(yī)政司2011-873)
【分類號】：R749.4

【參考文獻】

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本文編號：1995352