本文選題：抑郁癥　切入點：模型　出處：《中國比較醫(yī)學雜志》2017年04期

【摘要】：目的海馬內(nèi)微量注射K252a,研究其對大鼠行為學及抑郁樣經(jīng)典指標的影響,擬建立一種新型抑郁癥動物模型。方法 SD大鼠隨機分為5組,即空白對照組、假手術(shù)組、慢性應(yīng)激抑郁模型組、海馬內(nèi)微量注射K252a組、海馬內(nèi)微量注射K252a聯(lián)合慢性應(yīng)激組。采用Open field實驗、糖水消耗實驗、Morris水迷宮測定大鼠行為學的影響,ELISA法檢測大鼠血清單胺遞質(zhì)含量的變化,放射免疫法觀察大鼠血漿CRH、ACTH、CORT含量的變化,Western blotting觀察大鼠海馬BDNF、CREB、ERK1/2、BCL-2蛋白表達的變化。結(jié)果與空白對照組比較,CUMS組大鼠的活動量、糖水消耗量、學習記憶能力顯著降低(P0.05或P0.01),HPA軸功能亢進(P0.01),血清單胺遞質(zhì)水平降低(P0.01),BDNF、CREB、ERK1/2、BCL-2的表達下調(diào)(P0.01或P0.05),而DMSO組上述指標差異無統(tǒng)計學意義;與DMSO比較,K252a組、K252a+CUMS組活動量、糖水消耗量、學習記憶能力顯著下降(P0.01或P0.05),血清單胺遞質(zhì)水平下降(P0.01或P0.05),HPA軸功能顯著上升(P0.01或P0.05),海馬BDNF、CREB、ERK1/2、BCL-2的表達顯著下降(P0.01或P0.05);與CUMS組比較,K252a組、K252a+CUMS組大鼠上述指標差異無顯著性;與K252a組比較,K252a+CUMS組大鼠上述指標差異無顯著性。結(jié)論從行為學、血液學、分子生物學的不同角度分析表明,此模型與經(jīng)典的慢性應(yīng)激抑郁模型在表面效度、結(jié)構(gòu)效度、功能效度上具有極大的相似性,可為病因研究和抗抑郁藥物篩選提供一個可供選擇的研究技術(shù)平臺。
[Abstract]:Objective to study the effect of intrahippocampal microinjection of K252a on behavioral and depression-like classic indexes in rats and to establish a new type of depression animal model.Methods SD rats were randomly divided into 5 groups: blank control group, sham operation group, chronic stress depression model group, intrahippocampal microinjection K252a group, intrahippocampal microinjection K252a combined with chronic stress group.Open field test and Morris water maze test were used to determine the behavior of rats. The changes of serum monoamine transmitters in rats were detected by Elisa.Radioimmunoassay was used to observe the changes of plasma CRHN-ACTHH-CORT in rats and the expression of BDNF-CREBERK1 / 2 BCL-2 protein in rat hippocampus was observed by Western blotting.Results compared with the blank control group, the activity and sugar water consumption of rats in CUMS group were compared.The ability of learning and memory decreased significantly (P0.01 or P0.05), the level of serum monoamine transmitters decreased P0.01 or P0.05, the function of HPA axis increased significantly (P0.01 or P0.05), and the expression of BCL-2 in hippocampal BDNF CREBEBERK1 / 2 + BCL-2 decreased significantly (P0.01 or P0.05), but there was no significant difference between K252a group and K252a group (P0.01 or P0.05).There was no significant difference between K252a CUMS group and K252a group.Conclusion from different angles of behavior, hematology and molecular biology, this model is similar to the classical chronic stress depression model in surface validity, structural validity and functional validity.It can provide an alternative research technology platform for etiology research and antidepressant drug screening.
【作者單位】： 湖南中醫(yī)藥大學;湖南省中藥粉體與創(chuàng)新藥物省部共建國家重點實驗室培育基地;湖南省腫瘤醫(yī)院;湖南中醫(yī)藥大學第一附屬醫(yī)院;
【基金】：國家自然科學基金青年項目(81403387) 湖南省教育廳一般項目(14C0862;15C1038) 湖南省科技廳重點項目(2015DK3003) 中醫(yī)內(nèi)科學省部共建教育部重點實驗室開放基金(ZYNK201503)
【分類號】：R749.4

【參考文獻】

相關(guān)期刊論文 前6條

1 薛濤;鄔麗莎;劉新民;王瓊;;抑郁癥動物模型及評價方法研究進展[J];中國實驗動物學報;2015年03期

2 Hongyan Wang;Yingquan Zhang;Mingqi Qiao;;Mechanisms of extracellular signal-regulated kinase/cAMP response element-binding protein/brain-derived neurotrophic factor signal transduction pathway in depressive disorder[J];Neural Regeneration Research;2013年09期

3 王丹;;抑郁癥動物模型及行為學評價[J];西安文理學院學報(自然科學版);2012年02期

4 嚴華成;曹雄;Manas Das;朱心紅;高天明;;抑郁癥動物模型的研究進展(英文)[J];Neuroscience Bulletin;2010年04期

5 ;Role of brain-derived neurotrophic factor and neuronal nitric oxide synthase in stress-induced depression[J];Neural Regeneration Research;2008年04期

6 袁華;龍華;牟翔;李玲;;兩種抑郁模型的行為學比較[J];中國臨床康復(fù);2006年06期

【共引文獻】

相關(guān)期刊論文 前3條

1 徐偉超;顧劍潔;葛蓉;劉麗均;徐平;芮榮;;C57BL/6J小鼠凍融胚胎移植后所得小鼠及其子代生物學特性數(shù)據(jù)測定與分析[J];中國比較醫(yī)學雜志;2017年04期

2 任建枝;蔡嘉力;韓冬梅;李萍;耿潔;馬彩輝;韓艷;;胚胎培養(yǎng)液中血管內(nèi)皮生長因子的水平及其與IVF-ET結(jié)局的關(guān)系[J];東南國防醫(yī)藥;2016年05期

3 劉先菊;王艷蓉;高子琪;代小偉;佟巍;張麗芳;劉云波;;近交系小鼠微衛(wèi)星DNA引物的篩選和Tm值優(yōu)化研究[J];實驗動物科學;2012年01期

【二級參考文獻】

相關(guān)期刊論文 前10條

1 于瑞杰;王鋒;汪俊軍;;生命早期生長狀況對成人期餐后代謝反應(yīng)及相關(guān)疾病的影響[J];醫(yī)學研究生學報;2014年06期

2 王強;杭濤;徐云;程訓民;張啟高;宮劍濱;王t，

本文編號：1720759