本文選題：脂質(zhì)體包埋　切入點(diǎn)：腦源性神經(jīng)營養(yǎng)因子　出處：《中國免疫學(xué)雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的:對(duì)脂質(zhì)體包埋腦源性神經(jīng)營養(yǎng)因子進(jìn)行一定的修飾,觀察其透過血腦屏障的組織分布特點(diǎn)。方法:選取西安外事學(xué)院醫(yī)學(xué)院進(jìn)行實(shí)驗(yàn)的SD大鼠32只作為研究對(duì)象,隨機(jī)數(shù)字法分為脂質(zhì)體組(n=16)和對(duì)照組(n=16)。建立AD大鼠模型,對(duì)照組大鼠不給予任何處理,脂質(zhì)體組經(jīng)大鼠尾靜脈注射10μg/kg Tf-p GFAP-BDNF-PEG,分析脂質(zhì)體包埋腦源性神經(jīng)營養(yǎng)因子靶向脂質(zhì)體透過血腦屏障的組織分布特點(diǎn)。結(jié)果:采用24m Ci99m TC標(biāo)記1 ml神經(jīng)生長因子,取20μl上柱分離后采集40~50管收集液,每管0.5 ml,測定放射性技術(shù)。將峰所在的8~17管收集液混合回收,結(jié)果顯示神經(jīng)營養(yǎng)因子標(biāo)記率為98.9%。脂質(zhì)體組第10、14天腦源性神經(jīng)營養(yǎng)因子免疫陽性表達(dá)細(xì)胞數(shù),顯著高于對(duì)照組(P0.05);脂質(zhì)體組大鼠腦組織中神經(jīng)營養(yǎng)因子的基因表達(dá)第14天時(shí),顯著高于對(duì)照組(P0.05);脂質(zhì)體組大鼠存在神經(jīng)營養(yǎng)因子表達(dá),并且神經(jīng)營養(yǎng)因子表達(dá)水平顯著高于對(duì)照組(P0.05)。肉眼觀察所采腦脊液清亮、無色、透明,且顯微鏡下未檢出紅細(xì)胞。脂質(zhì)體組大鼠腦脊液便隱血膠體金檢測結(jié)果顯示陰性,對(duì)照組為陽性。結(jié)論:轉(zhuǎn)鐵蛋白聯(lián)合聚乙二醇修飾的脂質(zhì)體合并連接腦特異性啟動(dòng)子,能提高脂質(zhì)體靶向性。
[Abstract]:Objective: to study the tissue distribution of brain-derived neurotrophic factor (BDNF) embedded in liposome. Methods: Thirty-two Sprague-Dawley rats (SD rats) were selected as experimental subjects. The rat model of AD was established, and the control group was not treated with any treatment. In the liposome group, 10 渭 g / kg Tf-p GFAP-BDNF-PEG was injected into the tail vein of rats, and the tissue distribution characteristics of the liposome entrapped brain-derived neurotrophic factor targeted liposomes through the blood-brain barrier were analyzed. Results: 1 ml nerve growth factor (NGF) was labeled with 24m Ci99m TC. After the separation on 20 渭 l column, 40 ~ 50 tubes of collecting liquid were collected, 0.5 ml per tube, and radioactivity was determined by radioactivity technique. The results showed that the labeling rate of neurotrophic factor was 98.9%. The number of brain-derived neurotrophic factor immunoreactive cells in the liposome group was significantly higher than that in the control group on the 14th day, and the expression of neurotrophic factor gene in the brain tissue of the liposome group was higher than that of the control group on the 14th day. The expression of neurotrophic factor in liposome group was significantly higher than that in control group, and the expression level of neurotrophic factor in liposome group was significantly higher than that in control group (P 0.05). The cerebrospinal fluid collected by naked eye observation was clear, colorless and transparent. No erythrocytes were detected under microscope. The results of colloidal gold in cerebrospinal fluid (CSF) of rats in liposome group were negative and those in control group were positive. Conclusion: transferrin combined with polyethylene glycol modified liposome combined with brain-specific promoter. It can improve the targeting of liposome.
【作者單位】： 西安外事學(xué)院醫(yī)學(xué)院;
【基金】：陜西省教育廳基金項(xiàng)目(16JK2172)
【分類號(hào)】：R749.16

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