本文關鍵詞： 白藜蘆醇 脂多糖 PC12細胞 RT-PCR 炎性細胞因子　出處：《昆明醫(yī)科大學》2013年碩士論文　論文類型：學位論文

【摘要】：目的：通過構建脂多糖(Lipopolysaccharide, LPS)誘導的大鼠腎上腺嗜鉻細胞瘤細胞株PC12細胞炎癥模型,建立阿爾茨海默病的體外模型；從細胞、分子層面探討白藜蘆醇(Resveratrol,RES)對PC12細胞炎癥的保護作用。方法：體外培養(yǎng)的PC12細胞隨機分成空白對照組、LPS (1mg/ml,18h)處理組、不同濃度的白藜蘆醇(25、50和100μM,1h)+LPS處理組。采用免疫熒光雙標記檢測方法(Double-immunofluorescence Labeling)在熒光顯微鏡下觀察細胞內白介素-1β(interleukin-1β, IL-1β)和腫瘤壞死因子-α(tumor necrosis factor-α, TNF-α)的表達變化；應用逆轉錄聚合酶鏈式反應(reverse transcription polymerase chain reaction, RT-PCR)在mRNA水平檢測TNF-α和IL-1p的表達變化。 結果：不同濃度的白藜蘆醇(25、50和100μM)在翻譯和轉錄水平明顯抑制了LPS誘導的PC12細胞炎性細胞因子IL-1β和TNF-α的表達,并呈現(xiàn)濃度依賴性抑制。 結論：白藜蘆醇能夠抑制脂多糖誘導的PC12細胞炎性細胞因子IL-1β和TNF-α的釋放,從而發(fā)揮其抗神經炎癥的作用。
[Abstract]:Objective: to establish an in vitro model of Alzheimer's disease in rat adrenal pheochromocytoma cell line PC12 cells induced by lipopolysaccharide (LPS). To investigate the protective effect of resveratrol resveratrolon resveratrol on inflammation of PC12 cells at the molecular level. Methods: PC12 cells cultured in vitro were randomly divided into two groups: the control group treated with 1 mg / ml of resveratrol for 18 h. The expression of interleukin-1 尾 interleukin-1 尾 interleukin-1 尾 (IL-1 尾) and tumor necrosis factor- 偽 (TNF- 偽) were observed under fluorescence microscope in different concentration groups treated with resveratrol 2550 and 100 渭 m for 1 h) LPS. Reverse transcription polymerase chain reaction (RT-PCRR) was used to detect the expression of TNF- 偽 and IL-1p at mRNA level. Results: different concentrations of resveratrol 2550 and 100 渭 M inhibited the expression of PC12 inflammatory cytokines IL-1 尾 and TNF- 偽 in a dose-dependent manner at the translation and transcription levels. Conclusion: resveratrol can inhibit the release of inflammatory cytokines IL-1 尾 and TNF- 偽 in PC12 cells induced by lipopolysaccharide, thus exerting its anti-neuritis effect.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R749.16

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相關期刊論文 前1條

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本文編號：1517629