腎上腺髓質(zhì)素在急性壞死性胰腺炎大鼠肝、腎的表達(dá)
發(fā)布時(shí)間：2019-04-10 07:17
【摘要】：目的觀察腎上腺髓質(zhì)素（ADM）在急性壞死性胰腺炎（ANP）并肝、腎損傷大鼠肝、腎組織中的表達(dá)情況，觀察ADM在血清內(nèi)含量的變化情況，探討ADM與ANP及其合并的肝、腎損傷的關(guān)系。 方法將64只SD大鼠隨機(jī)分為ANP模型組（A組，n=32）和假手術(shù)對(duì)照組（C組，n=32）。A組采用經(jīng)十二指腸乳頭逆行胰膽管注射5%�；悄懰徕c誘導(dǎo)大鼠ANP并肝、腎損傷模型。C組僅開腹后輕輕翻動(dòng)胰腺。每組再分為3h、6h、12h、24h四個(gè)實(shí)驗(yàn)小組，每個(gè)實(shí)驗(yàn)小組8只SD大鼠，在術(shù)后對(duì)應(yīng)時(shí)點(diǎn)分批處死，，取胰腺、肝及腎組織在光鏡下進(jìn)行組織病理學(xué)檢查及病理學(xué)評(píng)分。分裝血清分別應(yīng)用底物酶法檢測(cè)淀粉酶（AMS）、丙氨酸氨基轉(zhuǎn)移酶（ALT）、天門冬氨酸氨基轉(zhuǎn)移酶（AST），應(yīng)用ELISA法檢測(cè)血清ADM濃度。應(yīng)用熒光定量PCR法檢測(cè)肝、腎組織內(nèi)ADM mRNA的表達(dá)。應(yīng)用免疫組化法檢測(cè)肝、腎組織內(nèi)ADM蛋白的表達(dá)情況。 結(jié)果A組大鼠胰腺病理?yè)p傷逐漸加重，病理評(píng)分逐漸增高，24h為最高值，各時(shí)點(diǎn)均顯著高于同時(shí)點(diǎn)C組（P＜0.01）。A組肝臟病理評(píng)分于造模后3h開始增高，12h達(dá)峰值，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.01）。A組腎臟病理評(píng)分逐漸增高，24h為最高值，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.05）。A組血清AMS與AST水平于造模后3h開始升高，6h達(dá)峰值，各時(shí)點(diǎn)均顯著高于同時(shí)點(diǎn)C組（P＜0.05, P＜0.01）。A組血清ALT水平逐漸升高，24h為最高值，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.05）。A組血清ADM水平于造模后3h上升，12h達(dá)峰值，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.05）。A組肝組織ADMmRNA表達(dá)于造模后3h升高，6h達(dá)峰值，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.05）。A組腎組織ADM mRNA表達(dá)于造模后12h內(nèi)升高不明顯，隨著時(shí)間延長(zhǎng)而緩慢上升，造模后24h為最高值，高于同時(shí)點(diǎn)C組（P＜0.01)。A組肝組織于造模后3h開始出現(xiàn)ADM蛋白強(qiáng)陽(yáng)性表達(dá)，24h為最高值，在3h、12h、24h時(shí)高于同時(shí)點(diǎn)C組（P＜0.05）。A組腎組織于造模后3h開始出現(xiàn)ADM蛋白強(qiáng)陽(yáng)性表達(dá)，6h達(dá)峰值，并一直保持在高水平，各時(shí)點(diǎn)均高于同時(shí)點(diǎn)C組（P＜0.05）。 結(jié)論ANP大鼠血清ADM水平升高可能與疾病嚴(yán)重程度有關(guān)，ADM在ANP大鼠肝、腎組織的表達(dá)增高可能與ANP合并的肝、腎損傷的嚴(yán)重程度有關(guān)。
[Abstract]:Objective to observe the expression of adrenomedullin (ADM) in the liver and kidney of rats with acute necrotizing pancreatitis (ANP) and renal injury, to observe the changes of ADM in serum, and to explore the relationship between ADM and ANP and their combined liver, and to observe the expression of adrenomedullin in liver and kidney of rats with acute necrotizing pancreatitis. The relationship between renal injury and renal injury. Methods Sixty-four SD rats were randomly divided into ANP model group (group A, n = 32) and sham-operated control group (group C, n = 32). ANP and liver were induced by retrograde injection of 5% sodium taurocholate into the duodenal papillary cholangiopancreatic duct in group C (n = 32). Renal injury model. Group C only slightly flipped the pancreas after laparotomy. Each group was divided into four groups (3 h, 6 h, 12 h, 24 h). Eight SD rats in each group were killed at the corresponding time point after operation. The pancreas, liver and kidney tissues were taken for histopathological examination and pathological score under light microscope. Serum (AMS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were detected by substrate enzyme method and serum ADM concentration was determined by ELISA method. The expression of ADM mRNA in liver and kidney was detected by fluorescence quantitative PCR. Immunohistochemical method was used to detect the expression of ADM protein in liver and kidney. Results the pathological injury of pancreas in group A was gradually aggravated, and the pathological score was gradually increased, the highest value was at 24 h, which was significantly higher than that in group C at the same time point (P < 0 01). The liver pathological score of group A was increased at 3 h after modeling, and reached the peak at 12 h after the establishment of the model (P < 0 01). The levels of serum AMS and AST in group C were significantly higher than those in group C at all time points (P < 0.01), and the renal pathological scores in group C were higher than those in group C at 24 hours (P < 0.01). The levels of serum AMS and AST in group C were significantly higher than those in group C at the same time (P < 0.05). A, P < 0.05). The levels of serum ALT in group C were significantly higher than those in group C at 6 h (P < 0.05, P < 0.01), and the highest level of serum ALT was at 24 h in group C (P < 0.05, P < 0.01). The levels of serum ADM in group C were higher than those in group C at the same time point (P < 0.05), the level of serum ADM in group). A increased at 3 h, reached the peak at 12 h, and the expression of ADMmRNA in liver tissue of group C at the same time point was higher than that in group C (P < 0.05). A) at 3 h after modeling. At 6 h, the expression of ADM mRNA in renal tissue of group C was higher than that of group C at the same time (P < 0.05). The expression of ADM mRNA in renal tissue of group C was not significantly increased within 12 hours after modeling (P < 0.05), but it increased slowly with the prolongation of time, and reached the highest value at 24 hours after modeling. The expression of ADM protein in liver tissue of group C was higher than that of group C at the same time (P < 0.01). The expression of ADM protein in liver tissue of group). A was the highest at 3 h and 12 h after the establishment of the model, and it was the highest at 24 h. The expression of ADM protein in renal tissue of group C was significantly higher than that of group C (P < 0.05) at 24 h (P < 0.05), and reached the peak at 6 h after the establishment of the model (P < 0.05). The expression of ADM protein in renal tissue of group C was higher than that of group C at the same time point (P < 0.05). Conclusion the increase of serum ADM level in ANP rats may be related to the severity of the disease. The increased expression of ADM in the liver and kidney tissues of ANP rats may be related to the severity of liver and kidney injury associated with ANP.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R657.51
本文編號(hào)：2455603
[Abstract]:Objective to observe the expression of adrenomedullin (ADM) in the liver and kidney of rats with acute necrotizing pancreatitis (ANP) and renal injury, to observe the changes of ADM in serum, and to explore the relationship between ADM and ANP and their combined liver, and to observe the expression of adrenomedullin in liver and kidney of rats with acute necrotizing pancreatitis. The relationship between renal injury and renal injury. Methods Sixty-four SD rats were randomly divided into ANP model group (group A, n = 32) and sham-operated control group (group C, n = 32). ANP and liver were induced by retrograde injection of 5% sodium taurocholate into the duodenal papillary cholangiopancreatic duct in group C (n = 32). Renal injury model. Group C only slightly flipped the pancreas after laparotomy. Each group was divided into four groups (3 h, 6 h, 12 h, 24 h). Eight SD rats in each group were killed at the corresponding time point after operation. The pancreas, liver and kidney tissues were taken for histopathological examination and pathological score under light microscope. Serum (AMS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were detected by substrate enzyme method and serum ADM concentration was determined by ELISA method. The expression of ADM mRNA in liver and kidney was detected by fluorescence quantitative PCR. Immunohistochemical method was used to detect the expression of ADM protein in liver and kidney. Results the pathological injury of pancreas in group A was gradually aggravated, and the pathological score was gradually increased, the highest value was at 24 h, which was significantly higher than that in group C at the same time point (P < 0 01). The liver pathological score of group A was increased at 3 h after modeling, and reached the peak at 12 h after the establishment of the model (P < 0 01). The levels of serum AMS and AST in group C were significantly higher than those in group C at all time points (P < 0.01), and the renal pathological scores in group C were higher than those in group C at 24 hours (P < 0.01). The levels of serum AMS and AST in group C were significantly higher than those in group C at the same time (P < 0.05). A, P < 0.05). The levels of serum ALT in group C were significantly higher than those in group C at 6 h (P < 0.05, P < 0.01), and the highest level of serum ALT was at 24 h in group C (P < 0.05, P < 0.01). The levels of serum ADM in group C were higher than those in group C at the same time point (P < 0.05), the level of serum ADM in group). A increased at 3 h, reached the peak at 12 h, and the expression of ADMmRNA in liver tissue of group C at the same time point was higher than that in group C (P < 0.05). A) at 3 h after modeling. At 6 h, the expression of ADM mRNA in renal tissue of group C was higher than that of group C at the same time (P < 0.05). The expression of ADM mRNA in renal tissue of group C was not significantly increased within 12 hours after modeling (P < 0.05), but it increased slowly with the prolongation of time, and reached the highest value at 24 hours after modeling. The expression of ADM protein in liver tissue of group C was higher than that of group C at the same time (P < 0.01). The expression of ADM protein in liver tissue of group). A was the highest at 3 h and 12 h after the establishment of the model, and it was the highest at 24 h. The expression of ADM protein in renal tissue of group C was significantly higher than that of group C (P < 0.05) at 24 h (P < 0.05), and reached the peak at 6 h after the establishment of the model (P < 0.05). The expression of ADM protein in renal tissue of group C was higher than that of group C at the same time point (P < 0.05). Conclusion the increase of serum ADM level in ANP rats may be related to the severity of the disease. The increased expression of ADM in the liver and kidney tissues of ANP rats may be related to the severity of liver and kidney injury associated with ANP.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R657.51
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