【摘要】：目的:觀察運動訓(xùn)練對TBI后大鼠空間學(xué)習(xí)記憶能力的影響,對TBI大鼠模型進行規(guī)律足量運動訓(xùn)練后,觀察各組大鼠的水迷宮表現(xiàn)來探討運動訓(xùn)練對學(xué)習(xí)記憶能力的影響。通過研究大鼠海馬區(qū)BDNF的表達情況,探討運動訓(xùn)練影響TBI大鼠學(xué)習(xí)記憶能力的作用機制。方法:成年雄性SD大鼠45只,隨機分為對照組(假手術(shù)+非運動訓(xùn)練)、創(chuàng)傷組(手術(shù)+非運動訓(xùn)練)和創(chuàng)傷訓(xùn)練組(手術(shù)+運動訓(xùn)練),每組15只。創(chuàng)傷組和創(chuàng)傷訓(xùn)練組建立TBI模型,TBI后第4天,對創(chuàng)傷訓(xùn)練組進行運動訓(xùn)練(網(wǎng)屏訓(xùn)練、平衡木訓(xùn)練和游泳訓(xùn)練),每一項目每天訓(xùn)練2次,每次10min,持續(xù)2周。運動訓(xùn)練結(jié)束后,對各組大鼠行Morris水迷宮實驗,以評估大鼠的空間學(xué)習(xí)記憶能力的變化,記錄第1-4天大鼠逃避潛伏期時間和第5天大鼠穿越平臺次數(shù)。水迷宮實驗結(jié)束以后,處死大鼠,獲取大腦海馬組織,采用免疫組織化學(xué)方法對大鼠海馬組織CA1區(qū)腦源性神經(jīng)營養(yǎng)因子(BDNF)的陽性細胞進行染色,并測其陽性細胞表達數(shù)來表示BDNF的表達情況。采用SPSS20.0版統(tǒng)計軟件分析實驗數(shù)據(jù),本研究計量資料以(`x±s)表示,組間比較采用單因素方差分析,P0.05為差異有統(tǒng)計學(xué)意義。結(jié)果:水迷宮實驗結(jié)果:定位航行實驗:與創(chuàng)傷組相比,對照組和創(chuàng)傷訓(xùn)練組大鼠第2天至第4天逃避潛伏期時間明顯縮短(P0.05),對照組和創(chuàng)傷訓(xùn)練組無明顯差異(P0.05)�？臻g探索實驗:對照組和創(chuàng)傷訓(xùn)練組大鼠穿越平臺次數(shù)明顯高于創(chuàng)傷組(P0.05),對照組和創(chuàng)傷訓(xùn)練組則無明顯差異(P0.05)。免疫組化結(jié)果顯示:對照組和創(chuàng)傷組BDNF表達無明顯差異(P0.05),創(chuàng)傷訓(xùn)練組BDNF表達明顯高于創(chuàng)傷組和對照組(P0.05)。結(jié)論:TBI后大鼠可出現(xiàn)學(xué)習(xí)的記憶能力的下降,而足量規(guī)律的運動訓(xùn)練則以可改善TBI所致學(xué)習(xí)記憶能力障礙,其作用機制可能與運動訓(xùn)練可以使大鼠海馬區(qū)BDNF表達增高有關(guān)。
[Abstract]:Aim: to observe the effect of exercise training on the spatial learning and memory ability of rats after TBI, and to observe the water maze performance of rats in each group after regular and adequate exercise training in TBI rats to explore the effect of exercise training on learning and memory ability. By studying the expression of BDNF in the hippocampus of rats, the mechanism of the effect of exercise training on the learning and memory ability of TBI rats was discussed. Methods: Forty-five adult male SD rats were randomly divided into three groups: the control group (sham operation non-exercise training), the trauma group (operation non-exercise training) and the trauma training group (operation training), with 15 rats in each group. The TBI model was established in the trauma group and the trauma training group. On the 4th day after TBI, the trauma training group was given exercise training (screen training, balance beam training and swimming training). Each item was trained twice a day for 10 minutes for 2 weeks. After exercise training, Morris water maze test was performed to evaluate the spatial learning and memory ability of rats, and to record the escape latency time of rats on day 1-4 and the times of crossing the platform on day 5. After the water maze experiment, the rats were killed and the hippocampal tissues were obtained. The positive cells of brain-derived neurotrophic factor (BDNF) in the CA1 region of the hippocampus of the rats were stained by immunohistochemical method. The number of positive cells was measured to indicate the expression of BDNF. The statistical software of SPSS20.0 was used to analyze the experimental data. The measurement data of this study were expressed as (x 鹵s), and the analysis of variance was used in the comparison between groups. The difference was statistically significant (P0.05). Results: the result of water maze experiment: compared with the trauma group, the escape latency of rats in the control group and the trauma training group was significantly shorter than that in the trauma group (P0.05). There was no significant difference between the control group and the trauma training group (P0.05). Space exploration experiment: the times of traversing the platform in the control group and trauma training group were significantly higher than those in the trauma group (P0.05), but there was no significant difference between the control group and the trauma training group (P0.05). Immunohistochemical results showed that there was no significant difference in BDNF expression between the control group and the trauma group (P0.05). The expression of BDNF in the trauma training group was significantly higher than that in the trauma group and the control group (P0.05). Conclusion: there is a decrease in learning memory ability after TBI in rats, while adequate exercise training can improve the learning and memory impairment induced by TBI. The mechanism may be related to the increase of BDNF expression in hippocampus of rats after exercise training.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R651.15

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