【摘要】：目的觀察骨髓間質(zhì)干細胞(BMSCs)對犬急性缺血腦組織的保護作用,并探討其可能的機制。方法將24只成年雜交犬隨機分為治療組及對照組,DSA引導(dǎo)下行自體血栓栓塞大腦中動脈閉塞缺血模型制作,并抽取骨髓提取BMSCs,傳代并給予4’,6-二脒基-2-苯基吲哚(DAPI)標(biāo)記;1周后開顱行多點腦內(nèi)注射BMSCs移植;移植后1周行腦DWI序列掃描,計算梗死灶體積;4周后將犬處死,每組隨機選擇6只動物取腦標(biāo)本行TTC染色測定梗死灶體積;另外6只動物進行HE染色、VG染色、TUNEL染色評價腦損傷情況;免疫熒光染色了解腦源性神經(jīng)營養(yǎng)因子(BDNF)、堿性成纖維生長因子(b FGF)、胰島素樣生長因子1(IGF-1)和血管內(nèi)皮生長因子(VEGF)的表達情況。結(jié)果治療組梗死灶內(nèi)廣泛存在DAPI陽性細胞。治療組的梗死灶體積明顯小于對照組,P0.01。治療組梗死灶范圍、梗死灶內(nèi)細胞壞死、膠質(zhì)增生和膠質(zhì)瘢痕均較對照組減輕。治療組凋亡細胞明顯少于對照組,P0.05。治療組細胞因子BDNF、BFGF、IGF-1和VEGF表達陽性的細胞均顯著多于對照組。結(jié)論腦梗死后給予BMSCs移植,BMSCs能存活并自行向梗死灶遷移,并發(fā)揮腦保護作用,其機制可能和分泌多種神經(jīng)營養(yǎng)因子有關(guān)。
[Abstract]:Objective to investigate the protective effect of bone marrow mesenchymal stem cell (BMSCs) on acute ischemic brain tissue in dogs and its possible mechanism. Methods Twenty-four adult hybrid dogs were randomly divided into treatment group and control group. BMSCs were extracted from bone marrow, then subcultured and labeled with 4-diamidine-2-phenylindole-indole (DAPI) for 1 week, then BMSCs was injected into the brain after craniotomy, and brain DWI sequence scanning was performed 1 week after transplantation, and the dogs were killed after 4 weeks of infarct volume calculation. The infarct volume was determined by TTC staining in 6 animals in each group, and the brain injury was evaluated by HE staining with VG staining and Tunel staining. The expression of (BDNF), basic fibroblast growth factor (b FGF),) insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) were detected by immunofluorescence staining. Results DAPI positive cells were found in infarct focus of treatment group. The infarct volume in the treatment group was significantly smaller than that in the control group (P 0.01). The infarct area, cell necrosis, glial hyperplasia and glial scar in the treatment group were less than those in the control group. The apoptotic cells in the treatment group were significantly lower than those in the control group (P 0.05). The positive expression of BDNF BFGFF-1 and VEGF in the treatment group was significantly higher than that in the control group. Conclusion BMSCs can survive and migrate to the infarct area after cerebral infarction, and exert the protective effect of brain. The mechanism may be related to the secretion of many neurotrophic factors.
【作者單位】： 江西省宜春市人民醫(yī)院神經(jīng)內(nèi)科;
【基金】：江西省科技計劃項目(20112BBG70085)
【分類號】：R-332;R743.33

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