本文選題：心肌梗死 + 血管新生�。� 參考：《中國循環(huán)雜志》2017年11期

【摘要】：目的:觀察利拉魯肽對急性心肌梗死大鼠心肌血管新生的影響及其心肌保護作用。方法:冠狀動脈結(jié)扎法建立大鼠急性心肌梗死模型,成功建模的大鼠隨機分為三組(每組6只):對照組、利拉魯肽低劑量組(LS組)和利拉魯肽高劑量組(HS組),另外取6只大鼠僅穿線,不結(jié)扎,作為假手術(shù)組。LS組和HS組建模后分別腹腔皮下注射利拉魯肽70μg/(kg·d)和140μg/(kg·d),假手術(shù)組和對照組均注射相應(yīng)生理鹽水,每天1次,共2周。4周后,超聲心動圖評估大鼠心臟結(jié)構(gòu)及功能,HE染色觀察心肌組織形態(tài)學(xué)變化,Masson染色計算膠原容積分數(shù)(CVF),免疫組織化學(xué)法檢測大鼠心肌梗死邊緣區(qū)微血管密度(MVD)及血管內(nèi)皮生長因子(VEGF)蛋白表達,蛋白免疫印跡(Western blot)法檢測VEGF蛋白含量。結(jié)果:4周后,對照組較假手術(shù)組左心室射血分數(shù)(LVEF)和左心室短軸縮短率(LVFS)均明顯減低,左心室舒張末期內(nèi)徑(LVEDd)、左心室收縮末期內(nèi)徑(LVESd)及CVF明顯增加(P均0.01),而MVD及VEGF蛋白水平差異無統(tǒng)計學(xué)意義(P0.05);與對照組相比,LS組和HS組LVEF、LVFS明顯升高(P0.01),LVEDd、LVESd降低(P0.05),CVF明顯下降(P0.01),MVD及VEGF蛋白水平顯著增加(P0.01);HS組較LS組LVEF、LVFS明顯升高(P0.01),LVEDd、LVESd和CVF下降(P0.05),MVD及VEGF蛋白水平增加(P0.01或P0.05)。結(jié)論:利拉魯肽可促進急性心肌梗死大鼠血管新生,可能與增加VEGF蛋白表達有關(guān),并可減少膠原沉積,從而改善左心室收縮功能,發(fā)揮心肌保護作用,且與劑量有一定關(guān)系。
[Abstract]:Aim: to observe the effect of liralutin on myocardial angiogenesis and myocardial protection in rats with acute myocardial infarction (AMI). Methods: rat models of acute myocardial infarction were established by coronary artery ligation. The rats were randomly divided into three groups (6 rats in each group: control group). The low dose group (LS group) and the high dose group (HS group) were selected. The other 6 rats were taken out without ligation. After modeling as sham-operation group, LS group and HS group, respectively, 70 渭 g/(kg d and 140 渭 g/(kg d of liraropeptide were injected subcutaneously into the abdominal cavity. The sham operation group and control group were injected with corresponding saline once a day for 2 weeks, 4 weeks later. Evaluation of Cardiac structure and function by Echocardiography in Rats the changes of myocardial histomorphology were observed by Masson staining and collagen volume fraction (CVFN) was calculated by Masson staining. Microvessel density (MVD) and intravascular density (MVD) were detected by immunohistochemical method in the marginal area of myocardial infarction in rats. The expression of skin growth factor (VEGF) protein, The content of VEGF protein was detected by Western blotting. Results after 4 weeks, the left ventricular ejection fraction (LVEF) and the left ventricular shortening rate (LVFS) in the control group were significantly lower than those in the sham-operated group. Left ventricular end-diastolic diameter (LVED), left ventricular end-systolic diameter (LVESd) and CVF increased significantly (P 0.01), but there was no significant difference in MVD and VEGF protein levels between LS group and HS group (P 0.05). The levels of MVD and VEGF protein were significantly increased in HS group than those in LS group. Compared with LS group, the levels of LVEDd and CVF in HS group were significantly higher than those in LS group. The levels of MVD and VEGF protein in HS group were significantly higher than those in LS group (P 0.01 or P 0.05). Conclusion: Lilaru peptide can promote angiogenesis in rats with acute myocardial infarction, which may be related to the increase of VEGF protein expression, decrease collagen deposition, improve left ventricular systolic function and exert myocardial protection.
【作者單位】： 中山大學(xué)附屬第五醫(yī)院心血管內(nèi)科;中山大學(xué)附屬第五醫(yī)院放射科;中山大學(xué)附屬第五醫(yī)院超聲科;
【分類號】：R-332;R542.22

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