本文選題：胰島素　切入點：NGF　出處：《中國修復重建外科雜志》2013年02期 　論文類型：期刊論文

【摘要】：目的制備NGF-胰島素復合凝膠并觀察其對大鼠深Ⅱ度燙傷創(chuàng)面愈合的影響。方法以卡波姆980為基質(zhì),分別加入NGF 4 000 U、胰島素800 U,制備胰島素凝膠、NGF凝膠、NGF-胰島素復合凝膠。觀察NGF-胰島素復合凝膠性狀,并行體外藥物釋放檢測。75只SPF級雄性Wistar大鼠,體重200～250 g,隨機分為正常對照組(A組)、糖尿病對照組(B組)、局部胰島素凝膠治療組(C組)、局部NGF凝膠治療組(D組)、局部NGF-胰島素復合凝膠治療組(E組),每組15只。B、C、D、E組大鼠采用一次性腹腔注射鏈脲佐菌素(55 mg/kg)制備1型糖尿病模型,A組注射相同劑量檸檬酸鈉緩沖液。造模成功后,采用恒溫水浴箱法于各組大鼠背部制備深Ⅱ度燙傷模型。A、B組創(chuàng)面外敷空白凝膠基質(zhì),C、D、E組對應外敷胰島素凝膠、NGF凝膠、NGF-胰島素復合凝膠,每天換藥1次。燙傷后觀察各組大鼠存活情況,于3、7、11、15、21 d觀察創(chuàng)面愈合情況并計算創(chuàng)面愈合率,各時間點每組處死3只大鼠取皮膚全層標本行組織學及免疫組織化學染色觀察。結(jié)果制備的NGF-胰島素復合凝膠清亮透明,保濕性及黏附性良好,易于涂抹及清洗。體外釋放檢測示NGF-胰島素復合凝膠釋藥時間可達24 h以上,且30 d內(nèi)穩(wěn)定性良好。各組大鼠均存活至實驗完成。燙傷后3 d各組創(chuàng)面無縮小,7、11、15、21 d時E組創(chuàng)面愈合率最高,B組最低,與其余各組比較以及E、B組間比較,差異均有統(tǒng)計學意義(P0.05)。組織學觀察示各時間點E組肉芽組織和膠原纖維生長均優(yōu)于其余各組。免疫組織化學染色示各組CD34、增殖細胞核抗原(proliferating cell nuclear antigen,PCNA)均于3 d開始表達,且隨時間延長陽性細胞逐漸增多;各時間點E組微血管密度及PCNA最高,B組最低,與其余各組比較以及E、B組間比較差異均有統(tǒng)計學意義(P0.05)。結(jié)論局部應用NGF-胰島素復合凝膠可顯著促進大鼠深Ⅱ度燙傷創(chuàng)面愈合。
[Abstract]:Objective to prepare NGF- insulin composite gel and observe its effect on wound healing of deep second degree scald in rats. Methods Carbomer 980 was used as the matrix. NGF 4 000 U and insulin 800 U were added to prepare the NGF- insulin composite gel. The characters of NGF- insulin compound gel were observed, and the drug release in vitro was performed in 75 male Wistar rats of SPF grade. Weighing 200g, they were randomly divided into normal control group (n = 15), diabetes control group (n = 15), local insulin gel group (n = 15), local insulin gel group (n = 15), group C (n = 15), local NGF gel group (n = 15) and group E (n = 15). Rats in group A were injected with sodium citrate buffer at the same dose by intraperitoneal injection of streptozotocin (55 mg / kg). The deep 鈪，

本文編號：1618003