旋毛蟲(chóng)及其蟲(chóng)源性蛋白對(duì)盲腸結(jié)扎穿孔誘導(dǎo)的小鼠噥毒癥的影響
本文選題:膿毒癥 切入點(diǎn):旋毛蟲(chóng) 出處:《南方醫(yī)科大學(xué)學(xué)報(bào)》2016年08期 論文類型:期刊論文
【摘要】:目的觀察旋毛蟲(chóng)及其蟲(chóng)源性蛋白對(duì)盲腸結(jié)扎穿孔(CLP)誘導(dǎo)的小鼠膿毒癥的影響。方法 80只雄性BALB/c小鼠隨機(jī)分為假手術(shù)組、CLP組、旋毛蟲(chóng)肌幼蟲(chóng)(ML)預(yù)感染組、旋毛蟲(chóng)肌幼蟲(chóng)蟲(chóng)體可溶性蛋白(SMP)處理組和排泄分泌蛋白(MES)處理組。ML預(yù)感染組于術(shù)前28 d經(jīng)口感染300條旋毛蟲(chóng)肌幼蟲(chóng),其余各組分別于術(shù)后30 min腹腔注射PBS或SMP(25μg/只)或MES(25μg/只)。觀察小鼠術(shù)后狀態(tài)和72 h生存率,檢測(cè)術(shù)后12 h小鼠血清中丙氨酸氨基轉(zhuǎn)移酶(ALT)、天門冬氨酸氨基轉(zhuǎn)移酶(AST)、尿素氮(BUN)和肌酐(Cr)水平及TNF-α、IL-6、IL-1β、IL-10、TGF-β水平,觀察小鼠肝和腎組織病變。結(jié)果與假手術(shù)組相比,CLP組72 h生存率降低,血清中ALT、AST、BUN和Cr水平及細(xì)胞因子TNF-α、IL-6、IL-1β、IL-10和TGF-β水平均明顯升高(P0.05)。肝中肝索排列紊亂,肝細(xì)胞水腫,腎中部分血管球皺縮,腎小管細(xì)胞水腫。與CLP組相比,ML預(yù)感染組血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,IL-10和TGF-β水平升高(P0.05);SMP處理組血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,TGF-β水平升高(P0.05);MES處理組72 h生存率明顯升高,血清中ALT、AST、BUN、Cr、TNF-α、IL-6和IL-1β水平明顯降低,IL-10和TGF-β水平明顯升高(P0.05),肝和腎組織結(jié)構(gòu)損傷明顯減輕。結(jié)論旋毛蟲(chóng)及其蟲(chóng)源性蛋白可減少CLP誘導(dǎo)的膿毒癥小鼠血清中促炎因子的釋放,促進(jìn)免疫調(diào)節(jié)因子的釋放,其中MES效果更為顯著,并能減輕肝和腎結(jié)構(gòu)和功能的損傷。
[Abstract]:Objective to observe the effect of Trichinella spiralis and its insect-derived protein on sepsis induced by cecal ligation and perforation in mice. Methods 80 male BALB/c mice were randomly divided into two groups: sham operation group and trichinella muscle larva preinfection group. The muscle larvae of Trichinella spiralis were treated with soluble protein (SMPP) and excretory and secretory protein (MESs). ML preinfection group was infected with 300 trichinella muscle larvae by mouth 28 days before operation. The other groups were injected intraperitoneally with PBS or SMP(25 渭 g / mouse or MES(25 渭 g / mouse at 30 min after operation. The postoperative state and 72 h survival rate of the mice were observed. The serum levels of alanine aminotransferase (alt), aspartate aminotransferase (ASTU), bun (bun), creatinine (Cr) and TNF- 偽 IL-6 尾 IL-10 TGF- 尾 in serum of mice were measured 12 hours after operation, and the pathological changes of liver and kidney were observed. Results the 72 h survival rate of CLP group was lower than that of sham CLP group. The levels of bun and Cr in serum and cytokines TNF- 偽, IL-6, IL-1 尾, IL-10 and TGF- 尾 in serum were significantly higher than those in control group (P 0.05). Renal tubular cell edema. Compared with CLP group, the serum levels of TNF- 偽 and TGF- 尾 in ML-preinfection group were decreased, the levels of IL-10 and TGF- 尾 were increased, the serum levels of TGF- 偽 and TGF- 尾 in serum of P0.05- SMPtreated group increased significantly, and the survival rate of TGF- 尾 increased significantly in P0.05MES treatment group. The levels of TNF- 偽 IL-6 and IL-1 尾 in serum of Asthma sinensis were significantly decreased, the levels of IL-10 and TGF- 尾 increased significantly, and the damage of liver and kidney tissue structure was alleviated. Conclusion Trichinella spiralis and its insect-derived protein can reduce the release of pro-inflammatory factor in serum of CLP induced sepsis mice. Promote the release of immunomodulators, MES is more effective, and can reduce the damage of liver and kidney structure and function.
【作者單位】: 蚌埠醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;蚌埠醫(yī)學(xué)院病原生物學(xué)教研室;蚌埠醫(yī)學(xué)院病理學(xué)教研室;蚌埠醫(yī)學(xué)院第二附屬醫(yī)院;蚌埠醫(yī)學(xué)院人體解剖學(xué)教研室;
【基金】:國(guó)家自然科學(xué)基金項(xiàng)目(81441120) 高?蒲袆(chuàng)新平臺(tái)團(tuán)隊(duì)項(xiàng)目(2016-40) 安徽省自然科學(xué)基金項(xiàng)目(1508085QH158) 安徽省高校優(yōu)秀青年人才支持計(jì)劃重點(diǎn)項(xiàng)目(gxyq ZD2016159) 安徽省高等學(xué)校自然科學(xué)研究重點(diǎn)項(xiàng)目(KJ2016A708) 安徽省自然科學(xué)青年科學(xué)基金項(xiàng)目(1608085QC51) 安徽省高等學(xué)校自然科學(xué)研究項(xiàng)目(KJ2015B013by) 蚌埠醫(yī)學(xué)院自然科學(xué)研究重點(diǎn)項(xiàng)目(BYKL1402ZD) 安徽省級(jí)大學(xué)生創(chuàng)新項(xiàng)目(201510367016)~~
【分類號(hào)】:R459.7
【相似文獻(xiàn)】
相關(guān)期刊論文 前3條
1 宋景春;胡敦重;王濤;陳自力;林兆奮;;BALB/c和昆明(KM)小鼠膿毒癥盲腸結(jié)扎穿孔模型的比較[J];實(shí)驗(yàn)動(dòng)物科學(xué);2012年03期
2 萬(wàn)梅,凌亦凌,黃善生,張君嵐,郝榮利;兔盲腸結(jié)扎穿孔早期肺動(dòng)脈壓增高中一氧化氮與內(nèi)皮素含量的變化[J];中國(guó)應(yīng)用生理學(xué)雜志;1997年02期
3 ;[J];;年期
相關(guān)會(huì)議論文 前3條
1 宋景春;胡敦重;陳自力;林兆奮;;盲腸結(jié)扎穿孔致小鼠彌漫性血管內(nèi)凝血的試驗(yàn)研究[A];江西省第六次中西醫(yī)結(jié)合心血管學(xué)術(shù)交流會(huì)論文集[C];2013年
2 夏季;王建東;任艷;趙康;徐寧志;徐迎新;劉斯奇;;盲腸結(jié)扎穿孔小鼠模型的血漿蛋白質(zhì)組分析[A];中國(guó)蛋白質(zhì)組學(xué)第二屆學(xué)術(shù)大會(huì)論文摘要論文集[C];2004年
3 周曉明;楊全會(huì);劉大為;;嚴(yán)重感染中大鼠不同臟器缺氧誘導(dǎo)因子—1α的表達(dá)及與臟器缺氧的關(guān)系[A];第三屆重癥醫(yī)學(xué)大會(huì)論文匯編[C];2009年
相關(guān)博士學(xué)位論文 前1條
1 王建東;小鼠盲腸結(jié)扎穿孔模型血漿蛋白質(zhì)組變化的初步研究[D];中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院;2005年
,本文編號(hào):1598264
本文鏈接:http://www.sikaile.net/yixuelunwen/jjyx/1598264.html