本文關(guān)鍵詞： 微小RNA- 烏司他丁 炎性反應(yīng)　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：目的:觀察在脂多糖(lipopolysaccharide,LPS)誘導(dǎo)的小鼠巨噬細(xì)胞株RAW264.7產(chǎn)生炎癥反應(yīng)中微小RNA-21(Micro RNA-21,mi R-21)的表達(dá)情況,以及烏司他丁(Ulinastatin,UTI)在炎癥反應(yīng)中對(duì)mi R-21的干預(yù)作用。方法:設(shè)置正常組、模型組(LPS 500 ng/m L剌激組)、實(shí)驗(yàn)組(LPS 500 ng/m L+UTI 1 000 U/m L組)和陰性實(shí)驗(yàn)對(duì)照組(UTI 1 000 U/m L),觀察RAW264.7細(xì)胞生長(zhǎng)狀態(tài);四甲基偶氮唑(methyl thiazolyl tetrazolium,MTT)法檢測(cè)不同濃度UTI對(duì)RAW264.7細(xì)胞活性的影響;用不同濃度UTI預(yù)處理2 h后,LPS刺激小鼠RAW264.7細(xì)胞誘導(dǎo)炎癥模型,RT-PCR檢測(cè)腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)m RNA、白介素-6(interleukin-6,IL-6)m RNA、mi R-21的表達(dá)。結(jié)果:(1)UTI對(duì)RAW264.7細(xì)胞的毒性作用:UTI濃度小于1 000 U/m L時(shí)對(duì)RAW264.7細(xì)胞無(wú)毒性作用,比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.117)。(2)與正常組相比,在不同濃度(100,250,500 ng/m L)LPS刺激RAW264.7細(xì)胞后,TNF-αm RNA及IL-6 m RNA的分泌(1.311±0.037,1.549±0.039,1.667±0.059;1.167±0.019,1.518±0.058,1.740±0.071)與基礎(chǔ)分泌量(1.000±0.000)相比明顯升高(P=0.000),且mi R-21表達(dá)(1.082±0.114,1.671±0.087,2.789±0.107)明顯升高,并隨LPS濃度升高而增加(P=0.000);(3)不同濃度UTI(10,100,1 000 U/m L)能有效降低TNF-αm RNA及IL-6 m RNA(1.000±0.000,1.998±0.212,1.421±0.100,1.122±0.154,0.991±0.139;1.000±0.000,1.880±0.045,1.179±0.108,1.053±0.070,0.960±0.088)的表達(dá),并降低mi R-21(1.000±0.000,2.789±0.107,2.675±0.135,2.258±0.170,1.369±0.279)的表達(dá),差異有統(tǒng)計(jì)學(xué)意義(P1=0.000,P2=0.000,P3=0.000)。結(jié)論:LPS刺激小鼠RAW264.7細(xì)胞可促進(jìn)mi R-21表達(dá)升高,其表達(dá)水平在一定程度上反映了細(xì)胞炎癥反應(yīng)狀態(tài);UTI可通過(guò)下調(diào)mi R-21抑制相關(guān)信號(hào)通路從而在免疫炎癥反應(yīng)中發(fā)揮保護(hù)作用。
[Abstract]:Aim: to observe the expression of minimal RNA-21(Micro RNA-21 in lipopolysaccharide lipopolysaccharide (LPS) -induced inflammatory reaction in murine macrophage cell line RAW264.7, and the effect of ulinastatin Ulinastatinatin UTII on the inflammatory response of murine macrophage cell line RAW264.7. Methods: normal control group was set up. The growth status of RAW264.7 cells was observed in the model group stimulated with LP500 ng/m L, the experimental group in the LP500 ng/m L UTI 1 000 U / mL group and the negative control group in the control group. The effects of different concentrations of UTI on the cell activity of RAW264.7 were detected by the method of tetrazoliumium tetrazolium tetrazolium tetrazolium (RAW264.7). After pretreatment with different concentrations of UTI for 2 h, the inflammatory model induced by RAW264.7 cells was pretreated with different concentrations of UTI. RT-PCR was used to detect the expression of tumor necrosis factor- 偽 -tumor necrosis factor- 偽 tNF- 偽 mRNAs and interleukin-6 interleukin-6m RNAi R-21. Results the toxic effect of UTI on RAW264.7 cells was less than 1 000 UMT L ~ (-1) 路min ~ (-1) ~ (-1) 路min ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1). It has no toxic effect on RAW264.7 cells. There was no significant difference between the two groups.) compared with the control group, the secretion of TNF- 偽 m RNA and IL-6 m RNA in RAW264.7 cells stimulated by LPs at different concentrations was significantly higher than that in the control group (1.311 鹵0.0377.49 鹵0.0399.1.667 鹵0.0591.167 鹵0.0191.518 鹵0.0581.740 鹵0.0771), and the expression of R-21 was 1.082 鹵0.1141.671 鹵0.082.789 鹵0.107), and the expression of R-21 was 1.082 鹵0.1141.671 鹵0.072.789 鹵0.107). With the increase of LPS concentration, the expression of TNF- 偽 m RNA and IL-6 m RNA(1.000 鹵0.000UmL) was significantly decreased. The difference was statistically significant (P < 0.01 0. 091 鹵0. 1391.000 鹵0. 1391.000 鹵0. 880 鹵0. 1081.179 鹵0. 1081.053 鹵0. 0700.60 鹵0. 088)), and decreased the expression of TNF- 偽 m RNA and IL-6 m RNA(1.000 鹵0. 10770 鹵2. 675 鹵0. 1352.258 鹵0. 1700.369 鹵0. 279). Conclusion LPS can stimulate the expression of TNF- 偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can stimulate the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can promote the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can promote the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 + 0.1070.675 鹵0.1352.258 鹵0.1752.258 鹵0.1700.369 鹵0.2799.Conclusion LPS can promote the expression of TNF- 偽 RNA and IL-6 m RNA(1.000 in mice. To a certain extent, the expression level of UUTI may play a protective role in the immune inflammatory response by down-regulating the signal pathway associated with the inhibition of miR-21.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院重癥醫(yī)學(xué)科;重慶市急救醫(yī)療中心ICU;
【基金】：重慶市衛(wèi)生計(jì)生委科研資助項(xiàng)目(編號(hào):2016ZDXM030)
【分類號(hào)】：R459.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 章雯靜;烏司他丁的臨床應(yīng)用進(jìn)展[J];中國(guó)新藥雜志;2003年06期

2 盧燕;袁家瑜;郎冰;;烏司他丁治療全身炎癥反應(yīng)綜合征療效觀察[J];寧夏醫(yī)學(xué)院學(xué)報(bào);2006年06期

3 高廣娟;;研究顯示:烏司他丁用于危重癥的證據(jù)進(jìn)一步完善[J];中國(guó)醫(yī)藥導(dǎo)報(bào);2014年03期

4 王長(zhǎng)安;;烏司他丁治療全身炎癥反應(yīng)綜合征的臨床觀察[J];中國(guó)藥物與臨床;2008年05期

5 楊新良;王雪松;劉瑋;;烏司他丁治療危重癥臨床療效觀察[J];海軍醫(yī)學(xué)雜志;2010年02期

6 趙秀芬;黃愛(ài)微;曹云云;李艷艷;;烏司他丁對(duì)胃癌患者術(shù)后肝腎功能保護(hù)作用的臨床觀察及護(hù)理[J];海峽藥學(xué);2009年04期

7 鄭翠英;張連祥;蔡忠;艾靜;;烏司他丁對(duì)胸外科手術(shù)患者圍手術(shù)期炎癥介質(zhì)的影響[J];江蘇醫(yī)藥;2010年03期

8 陳明法;;大劑量烏司他丁在重癥肺炎機(jī)械通氣治療中的療效分析[J];浙江創(chuàng)傷外科;2009年05期

9 何發(fā)標(biāo);;烏司他丁聯(lián)合血液凈化治療急性呼吸窘迫綜合征臨床分析[J];中華全科醫(yī)學(xué);2013年03期

10 石代紅;;烏司他丁對(duì)脂多糖所致內(nèi)毒素血癥大鼠胃腸黏膜循環(huán)的影響[J];中華普外科手術(shù)學(xué)雜志(電子版);2014年03期

相關(guān)會(huì)議論文 前2條

1 景炳文;;烏司他丁在急危重癥臨床應(yīng)用進(jìn)展[A];第十一次全國(guó)急診醫(yī)學(xué)學(xué)術(shù)會(huì)議暨中華醫(yī)學(xué)會(huì)急診醫(yī)學(xué)分會(huì)成立二十周年慶典論文匯編[C];2006年

2 陳鵬;祝益民;羅海燕;;烏司他丁對(duì)重癥手足口病患兒的肺保護(hù)作用[A];中華醫(yī)學(xué)會(huì)第十七次全國(guó)兒科學(xué)術(shù)大會(huì)論文匯編（下冊(cè)）[C];2012年

相關(guān)碩士學(xué)位論文 前4條

1 李春平;烏司他丁對(duì)膿毒癥患者的治療作用及其機(jī)制的研究[D];山東大學(xué);2016年

2 房啟占;烏司他丁對(duì)血小板激活介導(dǎo)Sepsis所致內(nèi)皮細(xì)胞損傷的作用研究[D];東南大學(xué);2016年

3 周思佳;烏司他丁對(duì)膿毒癥患者血Th1/Th2漂移的影響[D];大連醫(yī)科大學(xué);2015年

4 唐亞純;烏司他丁對(duì)尿源性SIRS豬的治療作用及對(duì)腎組織NF-κB表達(dá)的影響[D];南華大學(xué);2013年

，

本文編號(hào)：1503312