背景:我們前期研究發(fā)現(xiàn),三叉神經(jīng)脊束尾核（trigeminal nucleus caudalis,TNC）中酸敏感離子通道3（acid-sensing ion channel 3,ASIC3）參與了反復(fù)發(fā)作性偏頭痛的病理過(guò)程。神經(jīng)生長(zhǎng)因子（nerve growth factor,NGF）可以誘導(dǎo)許多疼痛疾病的痛覺(jué)敏化,并且可以調(diào)控ASIC3的表達(dá)。然而,NGF在慢性偏頭痛（chronic migraine,CM）中的作用尚不清楚。所以我們探究了CM大鼠TNC部位NGF的表達(dá),NGF對(duì)ASIC3的調(diào)控作用,及相關(guān)的信號(hào)通路在炎性湯（inflammatory soup,IS）誘導(dǎo)的皮膚痛覺(jué)過(guò)敏中的作用。方法:1.通過(guò)硬腦膜反復(fù)滴注7日IS建立CM大鼠模型,模擬CM的發(fā)作。von Frey測(cè)定大鼠眶周及足底的痛閾值,以此評(píng)價(jià)模型的建立。2.利用PCR,Western blot檢測(cè)TNC部位NGF m RNA水平及蛋白水平,免疫熒光檢測(cè)NGF在TNC神經(jīng)元中的定位情況。3.CM大鼠側(cè)腦室注射NGF中和抗體（anti-NGF neutralizing antibody,anti-NGF）,von... 

【文章來(lái)源】：重慶醫(yī)科大學(xué)重慶市

【文章頁(yè)數(shù)】：51 頁(yè)

【學(xué)位級(jí)別】：碩士

【部分圖文】：

Sham組和CM組的痛閾值

NGF在TNC部位的表達(dá)和定位Fig.2TheexpressionandlocalizationofNGFintheTNC(A)ThemRNAofNGFintheTNCwasdetectedwithq-PCR.Asshownbythestatisticalanalysis,theNGFmRNAexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(B)TheNGFproteinexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(n=6pergroup,*p<0.05,**p<0.01vs

圖 3.Anti-NGF 對(duì)痛閾值的影響Fig. 3 Effects of anti-NGF treatment on the pain thresholds(A) The periorbital pain threshold was significantly lower in the CM and CM+PBS groupan in the Sham group, and there was no significant difference between the CM and CM+Pgroups. The pain threshold of the periorbital region was significantly higher in theM+anti-NGF MD (0.5 g) and CM+anti-NGF HD (1 g) groups than in the CM+PBS grohere were no significant differences between the CM+anti-NGF MD and CM+anti-NGF Hgroups. The pain threshold of the periorbital region did not differ significantly between thCM+anti-NGF LD (0.1 g) group and the CM+PBS group. (B) The hind paw withdrawareshold was significantly lower in the CM and CM+PBS groups than in the Sham group, there was no significant difference between the CM and CM+PBS groups. The withdrawthreshold of the hind paw was significantly higher in the CM+anti-NGF MD (0.5 g) andCM+anti-NGF HD (1 g) groups than in the CM+PBS group. There were no significantifferences between the CM+anti-NGF MD and CM+anti-NGF HD groups. The pain threshf the hind paw did not differ significantly between the CM+anti-NGF LD (0.1 g) group athe CM+PBS group. (n=6 per group, ** p < 0.01 vs Sham group; ## p < 0.01 vs CM+PBS


本文編號(hào)：3353951