本文選題：華通膠 + 間充質(zhì)干細(xì)胞��； 參考：《中國(guó)實(shí)驗(yàn)血液學(xué)雜志》2013年01期

【摘要】：本研究旨在探討低溫保存對(duì)華通膠間充質(zhì)干細(xì)胞(WJ-MSC)生物學(xué)特性的影響,為低溫保存后WJ-MSC的臨床應(yīng)用及建立WJ-MSC庫提供實(shí)驗(yàn)依據(jù)。通過臍帶組織塊培養(yǎng)制備WJ-MSC,將第5代WJ-MSC加入低溫保護(hù)劑,-80℃冰箱降溫,液氮保存。細(xì)胞復(fù)溫后行培養(yǎng)和傳代,低溫保存組第1代(PP1)傳代獲得PP2-PP15。非低溫保存組第6代為對(duì)照組第1代(CP1),傳代獲得CP2-CP15。比較對(duì)照組與低溫保存組WJ-MSC的有核細(xì)胞回收率、臺(tái)盼藍(lán)拒染率、CCK-8活性、凋亡率、貼壁率、增殖指數(shù)、細(xì)胞表面抗原、細(xì)胞周期和向脂肪細(xì)胞、成骨細(xì)胞及神經(jīng)元細(xì)胞誘導(dǎo)分化能力。結(jié)果表明,WJ-MSC經(jīng)低溫保存,有核細(xì)胞回收率為98.2%,臺(tái)盼藍(lán)拒染率為94.3%,CCK-8活性為91.4%,凋亡率為3.9%,貼壁率為92.6%;PP1較CP1增殖指數(shù)明顯降低(P0.05),PP2-PP15與相應(yīng)對(duì)照組增殖指數(shù)的差異均無統(tǒng)計(jì)學(xué)顯著性;WJ-MSC高表達(dá)CD29、CD90、CD44、CD71、CD73、CD105、CD166和HLA-ABC,低表達(dá)CD34、CD45和HLA-DR,兩組之間差異均無統(tǒng)計(jì)學(xué)顯著性;增殖潛伏期和對(duì)數(shù)增殖期兩組之間差異也無統(tǒng)計(jì)學(xué)意義;向脂肪細(xì)胞、成骨細(xì)胞和神經(jīng)元細(xì)胞誘導(dǎo)分化后分別進(jìn)行油紅O、堿性磷酸酶(ALP)和神經(jīng)元特異性烯醇化酶(NSE)染色,兩組間陽性強(qiáng)度肉眼觀察無明顯差異;細(xì)胞內(nèi)甘油三酯、ALP和NSE含量?jī)山M間也無顯著差異。結(jié)論:WJ-MSC低溫保存后少量細(xì)胞(10%)受到損傷,傳代培養(yǎng)表明其生物學(xué)特性基本保持不變。
[Abstract]:The purpose of this study was to investigate the effect of cryopreservation on the biological characteristics of WJ-MSCC in Huatong Gel Mesenchymal Stem cells (MSCs), and to provide experimental evidence for the clinical application of cryopreserved WJ-MSC and the establishment of WJ-MSC library. WJ-MSCs were prepared by culture of umbilical cord tissue block. The fifth generation WJ-MSC was added into the cryogenic protectant (-80 鈩，

本文編號(hào)：1883559