本文選題：基底前腦 + 巢蛋白�。� 參考：《大理大學(xué)》2017年碩士論文

【摘要】：目的研究健康成年SD大鼠基底前腦巢蛋白(Nestin)陽性或陰性膽堿能神經(jīng)元神經(jīng)生長因子受體表達(dá)差異,探索兩種神經(jīng)元功能差異的可能機(jī)制。方法選取體質(zhì)量在200~250g,經(jīng)正常飼養(yǎng)的成年大鼠40只,不限性別,使用多聚甲醛灌注固定后用大鼠腦切片模具取腦,石蠟切片分為單標(biāo)組和雙標(biāo)組,以及對照組。單標(biāo)組是:Nestin、ChAT、TrkA,雙標(biāo)組是Nestin和TrkA;ChAT和TrkA組。對熒光雙標(biāo)組用激光共聚焦顯微鏡觀測三種神經(jīng)元在基底前腦共表達(dá)情況。使用免疫組織化學(xué)方法、顯微鏡檢測Nestin、ChAT、TrkA于基底前腦內(nèi)側(cè)隔核、斜角帶核垂直支、斜角帶核水平支分布以及陽性反應(yīng)物的表達(dá)數(shù)量。統(tǒng)計Nestin、TrkA、ChAT三種膽堿能神經(jīng)元在基底前腦相同區(qū)域(MS、vDB、hDB)的細(xì)胞數(shù)目、ChAT免疫陽性神經(jīng)元表達(dá)TrkA數(shù)目、Nestin陽性神經(jīng)元表達(dá)TrkA數(shù)目,推測出Nestin陰性神經(jīng)元表達(dá)TrkA數(shù)目,使用統(tǒng)計學(xué)均數(shù)±標(biāo)準(zhǔn)差(`X±S)進(jìn)行分析。結(jié)果實驗結(jié)果表明,在大鼠基底前腦MS-DBB區(qū)域有明顯TrkA陽性細(xì)胞,TrkA、Nestin膽堿能神經(jīng)元以及ChAT神經(jīng)元有相同區(qū)域共定位表現(xiàn),三種神經(jīng)元相間分布在大鼠基底前腦MS-DBB。數(shù)據(jù)顯示:ChAT免疫陽性神經(jīng)元數(shù)量高于Nestin陽性神經(jīng)元數(shù)量,Nestin幾乎完全雙標(biāo)ChAT;有大約90%以上的Nestin陽性神經(jīng)元表達(dá)TrkA,ChAT陽性神經(jīng)元表達(dá)TrkA的量約占70%左右,Nestin陰性神經(jīng)元也表達(dá)TrkA。結(jié)論通過熒光雙標(biāo)染色和激光共聚焦顯微鏡觀察得出,Nestin兩種神經(jīng)元能表達(dá)TrkA,但是巢蛋白的兩種神經(jīng)元表達(dá)TrkA數(shù)量是不同的,Nestin陽性神經(jīng)元表達(dá)TrkA的數(shù)量高于Nestin陰性神經(jīng)元表達(dá)TrkA的數(shù)量。證實巢蛋白的兩種神經(jīng)元表達(dá)神經(jīng)生長因子受體上存在差異,提示巢蛋白陽性神經(jīng)元能與更多的神經(jīng)生長因子結(jié)合,Nestin陽性或Nestin陰性兩種膽堿能神經(jīng)元在可塑性、興奮性、抗損傷功能等方面有差異,其機(jī)制可能是因為神經(jīng)生長因子受體表達(dá)量的不同而引起的。
[Abstract]:Objective to study the difference of nerve growth factor receptor (NGF) expression in cholinergic neurons of healthy adult SD rats with or without nestin (Nestin) and to explore the possible mechanism of the difference between the two neuronal functions. Methods Forty adult rats with a body weight of 200 ~ 250g were selected and were randomly divided into two groups: single labeled group, double labeled group and control group. The rats were fixed with paraformaldehyde and fixed with the molds of rat brain slice after infusing, and the paraffin sections were divided into single label group and double labeled group, as well as the control group. The single group was Nestin and TrkAc Chat TrkA, and the double labeled group was Nestin and TrkAn chat and TrkA group. The co-expression of three kinds of neurons in basal forebrain was observed by laser confocal microscope in double labeled group. Immunohistochemical method was used to detect the distribution of TrkA in the medial septal nucleus of the basal forebrain, the vertical branch of the diagonal band nucleus, the horizontal branch of the diagonal band nucleus and the expression of positive reactants. The number of the three kinds of cholinergic neurons in the same area of the basal forebrain, the number of chat immunoreactive neurons expressing TrkA and the number of nestin positive neurons expressing TrkA were counted, and the number of Nestin negative neurons expressing TrkA was inferred. Statistical mean 鹵standard deviation (`X 鹵S) was used to analyze. Results the results showed that there were significant TrkA positive cholinergic neurons and ChAT neurons in the MS-DBB region of the basal forebrain of rats. The three neurons were distributed in MS-DBB of the basal forebrain of rats. The data showed that the number of Nestin immunoreactive neurons was higher than that of Nestin positive neurons, and about 90% of the Nestin positive neurons expressed TrkA, and about 70% of the nestin negative neurons also expressed TrkA. Conclusion two kinds of nestin neurons can express TrkA by fluorescence double labeling and laser confocal microscopy, but the number of TrkA expression in nestin positive neurons is higher than that in Nestin negative neurons. The number of neurons expressing TrkA. It was confirmed that there were differences in the expression of nerve growth factor receptors between the two nestin neurons, suggesting that nestin positive neurons and more cholinergic neurons with nestin binding or Nestin negative neurons could exhibit plasticity and excitability. The mechanism may be due to the different expression of nerve growth factor receptor (NGF).
【學(xué)位授予單位】：大理大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R338

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